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Journal of Parasite Science

ISSN 2656-5331

Vol. 2 / No. 1 / Published : 2018-03

TOC : 8, and page :39 - 44

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Original Article :

Efectiveness of ocimum sanctum linn. leaves ethanol extract on mortality raillietina echinobothrida in vitro

Author :

  1. Jessica Giovani*1
  2. Setiawan Koesdarto*2
  3. Mirni Lamid*3
  4. Sri Agus Sudjarwo*4
  5. Muchammad Yunus*5
  6. Suryo Kuncorojakti*6
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan
  4. Dosen Fakultas Kedokteran Hewan
  5. Dosen Fakultas Kedokteran Hewan
  6. Dosen Fakultas Kedokteran Hewan

Abstract :

The aims of the research are to prove the anthelmintic effects of Ocimum sanctum Linn. leaves ethanol extract on Raillietina echinobothrida in vitro and to know the value of LC50 and LC90of Ocimum sanctum Linn. leaves ethanol extract. The research used completely randomized design. The research had five treatments and each treatment was done in four replications. The research used 5 Raillietina echinobothrida adult worms for each treatment and all replications. The observation and the recording were done at the first hour until the sixth hour of the research. The Raillietina echinobothrida adult worms were noted dead if there was no movement when they were disturbed by anatomic tweezer, the death of Raillietina echinobothrida were confirmed when they dipped mildly in warm water (50°C) and they showed no movements. The acquired data was analyzed using ANOVA and continued with LSD (5%). The results were Ocimum santum Linn. ethanol extract has the anthelmintic activity in 1.25%, 2.5%, 5% and 10% concentrations. The LC50 and LC90 was analyzed using probit analysis. The results of LC50 calculation were 7.8% at the third hour, 6% at the forth hour, 3.2% at the fifth hour, 2.5% at the sixth hour and the results of LC90 were 6.5% at the fifth hour, 5.2% at the sixth hour. The higher concentration of the extract, the higher anthelmintic activity worked.

Keyword :

: Ocimum sanctum Linn. leaves, ethanol extract, Raillietina echinobothrida, in vitro,

References :

  1. Asha, M. K., D. Parasanth, B. Murali, R. Padmaja and A. Amit, (2001). Anthelmintic Activity of Essential Oil of Ocimum sanctum and eugenol. Hal. 669-­‐670 : Elsevier Science B.V.: Fitoterapia
  2. Athanasiadou, S., I. Kyriazakis, F. Jackson, and R. L. Coop, (2001). Direct anthelmintic effects of condensed tannins towards different gastro¬in¬testinal nematodes of sheep in vitro and in vivo studies. Hal. 205–219 : Vet. Parasitol
  3. Hoste, H., J. Frank, A. Spiridoula, M. T. Stig and O. H. Simone. , (2006). The Effects of Tannin rich Plants on Parasitic Nematodes in Rumi¬nants. Vol. 22, No. 6 : Science Direct J. Trends in Parasitology


Archive Article

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Volume : 2 / No. : 1 / Pub. : 2018-03
  1. The prevalance and helminth infection degree of gastrointestinal in layer duck located in keper and markolak kramat village district of bangkalan regency of bangkalan
  2. The effect of calcium hydroxideon mortality of ascaridia galli in vitro
  3. The prevalance of gastrointestinal tract protozoa using fecal examination in local chicken(gallus domesticus) located in kramat village, district of bangkalan, bangkalan regency
  4. Prevalence and saverity level of scabies (sarcoptes scabiei) on rabbits in sajen village, pacet sub-district, mojokerto regency
  5. Anthelmintic activity ethanol extract of ocimum sanctum linn. leaves against ascaridia galli in vitro
  6. The conformity of diagnostic test between burrow ink test method with skin scrapping method of scabies in rabbit (lepus domesticus)
  7. Identification of worm in digestive tract of consumed cattle egret (bubulcus ibis) in ujung pangkah, gresik
  8. Efectiveness of ocimum sanctum linn. leaves ethanol extract on mortality raillietina echinobothrida in vitro
  9. Prevalence of nematodiasis in omasum and abomasum of goats slaughtered at pegirian surabaya slaughter house through gatrointestinal surgery method