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Journal Of Marine And Coastal Science

ISSN 2301-6159

Vol. 2 / No. 1 / Published : 2013-02

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Original Article :

Examination of iridovirus and vnn (viral nervous necrosis) in fish using pcr method in balai karantina ikan pengendalian mutu dan keamanan hasil perikanan (bkipm) kelas i ngurah rai, denpasar bali

Author :

  2. Hari Suprapto*2
  1. Mahasiswa Fakultas Perairan dan Kelautan
  2. Dosen Fakultas Perairan dan Kelautan

Abstract :

Current trend in aquaculture development today toward intensification and comersialisation of various fishery commodity actually very susceptible on spreading disease between the individu. Disease was the major factor that caused economic losses in aquaculture industry. Some disease caused by viruses in mariculture such as Iridovirus and Viral Nervous Necrosis (VNN). Virus easily spreading trough the water and very difficult to detect the existence. Viral disease potentially has a chance to spread through the border caused by effect of international trade and globalization.   The purpose of this Field Work Practice is to know, understand, and practice about examination process of Iridovirus and VNN using Polymerase Chain Reaction (PCR) method.             Working method that used in this Field Work Practice is descriptive research with collection of data included primary data and secondary data. Data were collected by active participation, observation, and interview. Field Work Practice carried out from 16th January until 16th February 2012 in Balai Karantina Ikan Pengendalian Mutu dan Keamanan Hasil Perikanan(Balai KIPM) Kelas I Ngurah Rai, Denpasar Bali.             Polymerase Chain Reaction is an enzymatic method that used to replicated exponentially a specific nucleotide sequence. Viral examination consist of 4 stage of process that is nucleic acid extraction, amplification, electrophoresis, and diagnosis the result. Using IQ2000 reagent kit from Farming IntelliGene Tech. Corp. Taiwan.             Examination result from 36 tiger grouper samples that enter Balai KIPM Ngurah Rai. Result shows that six samples of 18 samples were positive Iridovirus and three samples of 18 samples were positive VNN. The result determined by looking for band that appear in the electrophoresis gel. 

Keyword :

Iridovirus, Viral Nervous Necrosis (VNN, Polymerase Chain Reaction (PCR).,

References :

  1. F.A Rantam, (2005). Virologi. Surabaya : Airlangga University Press


Archive Article

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Volume : 2 / No. : 1 / Pub. : 2013-02
  1. Management of grass carp (ctenopharyngodon idella) hatchery in balai perbenihan dan budidaya ikan air tawar muntilan, muntilan, magelang
  2. Englargement techniques trench mud crab with pond method for silfofishery at ksu mekar nusantara ngudi mulya village, depok karang talun village, cilacap residence,central java province
  3. Application of good manufacturing practice (gmp) and sanitation standard operating procedure (ssop) for the production process canning lemuru (sardinella longiceps) in the cv. pacific harvest, banyuwangi regency-east java
  4. Hatchery of arwana silver brazil (osteoglossum bicirrhosum) in mina karya koi centre, district of sleman, province di. yogyakarta
  5. Improvement color quality of betta fish (betta splendens) with the addition of spirulina platensis on feed at biotech agro indonesia, jombang district, east java province
  6. Feeding alternative process made of basic earthworm (lumbricus sp.) in the form of flake and its application on fry catfish in upbat kepanjen – malang
  7. Examination of iridovirus and vnn (viral nervous necrosis) in fish using pcr method in balai karantina ikan pengendalian mutu dan keamanan hasil perikanan (bkipm) kelas i ngurah rai, denpasar bali

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