Majalah Farmasi Airlangga (Airlangga Journal of Pharmacy)
ISSN 0852-1050
Vol. 5 / No. 1 / Published : 2005-04
Related with : Scholar Yahoo! Bing
Original Article :
Bioautografi antibiotika hasil fermentasi mutan streptomyces griseus atcc 10137
Author :
- Isnaeni*1
- Bagian Kimia Farmasi Fakultas Farmasi Unair
Abstract :
Bioautography of a novel antibiotic produced by fermentation process of Streptomyces griseus ATCC 10137 had been investigated by using streptomycin standard. High Performance Thin Layer Chromatography had been carried out by using KH2PO4 5% solution as a solvent, and Bacillus subtilis PCI 219 as a bacterial test. Bioautogram showed that Rf value of spot derived from the antibiotic extract isolated from the fermentation filtrate is 0.65, which has significant different with Rf value of streptomycin standard 0.15. It was found that the antibiotic and streptomycin standard inhibited growth of Bacillus subtilis PCI 219.
Keyword :
Streptomyces griseus, bioautography, streptomycin,
References :
V. Betina,(1983) The chemistry and biology of antibiotics, Pharmaco-chemistry Library, 5 New York : Elsevier Scientific Publishing Company
Z.E. Buchanan,(1991) Bergey’s Manual of determinative bacteriology, 8th ed. Baltimore : Williams Wilkins Company
N.E. Gibbons,(1991) Bergey’s Manual of determinative bacteriology, 8th ed. Baltimore : Williams Wilkins Company
M.E. Bushell,(1988) Gwoth, product formation and fermentation technology, dalam Goodfellow (Ed), Actinomycetes in Biotechnology San Diego : Academic Press
P.J. Claes,(1974) Chromatographic analysis of neomycin. Isolation and identification of minor components, J. Antibiot., 27 :
F. Comperonelle,(1974) Chromatographic analysis of neomycin. Isolation and identification of minor components, J. Antibiot., 27 :
H. Vanderhaege,(1974) Chromatographic analysis of neomycin. Isolation and identification of minor components, J. Antibiot., 27 :
P.J. Claes,(1982) Thin-layer chromat
Archive Article
| Cover Media | Content |
|---|---|
 Volume : / No. : / Pub. : |
|
