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Majalah Farmasi Airlangga (Airlangga Journal of Pharmacy)

ISSN 0852-1050

Vol. 4 / No. 2 / Published : 2004-08

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Original Article :

Produksi enzim proteolitik sel amobil streptomyces griseus atcc 10137

Author :

  1. Achmad Toto Poernomo*1
  1. Fakultas Farmasi Universitas Airlangga Surabaya

Abstract :

Production of proteolitic enzyme was examined by immobilized cells of Streptomyces griseus ATCC 10137 in polyacrilamide gel. For immobilization 1 volume of 12% acrylamide and 0.70% bisacrylamide were used. To determine the proteolitic enzyme activity of immobilized and free cells were incubated in a batch fermentation in medium containing of 0.5% soluble starch, 0.5% lablemco extract, yeast extract, 0.02% CaCl2, 0.01% MgSO4 7H2O, pH 7.5 for 24 h. Recycle use of immobilized and free cells showed that, the enzyme proteolitic activity of the immobilized cells were gradually increased at the beginning of their used and reached maximum 15th use cycles. Then, gradually decreased with increasing recycle use. Whereas, the recycle use of free cells quickly increased at 5th use cycles with increasing repeated and quickly decrease with increasing use. Based on these date showed that immobilized cells have a better stability than their free cells for enzyme proteolitic production.

Keyword :

Immobilized, Streptomyces griseus, proteolitic enzyme, polyacrilamide gel,


References :

  1. P. Aleksieva, (1991). Acid proteinase production by Humicola lutea cells immobilized in polyhydroxyethylmethacrylate gel, Acta Biotech nol., 11. :
  2. E. Petricheva, (1991). Acid proteinase production by Humicola lutea cells immobilized in polyhydroxyethylmethacrylate gel, Acta Biotech nol., 11. :
  3. E. Konstantinov, (1991). Acid proteinase production by Humicola lutea cells immobilized in polyhydroxyethylmethacrylate gel, Acta Biotech nol., 11. :
  4. C. Robeva, (1991). Acid proteinase production by Humicola lutea cells immobilized in polyhydroxyethylmethacrylate gel, Acta Biotech nol., 11. :
  5. S. Mutafov, (1991). Acid proteinase production by Humicola lutea cells immobilized in polyhydroxyethylmethacrylate gel, Acta Biotech nol., 11. :
  6. G. Argarikos, (1992). Effect of immobilization on the production of -amylase by an industrial strain of Bacillus amyloliquefaciens, J. Chem. Tech. Biotechnol, vol 53. :
  7. K. Thayanithy, (1992). Effect of immobilization on the production of -amylase by an industrial strain of Bacillus amyloliquefaciens, J. Chem. Tech. Biotechnol, vol 53. :
  8. D.A.J. Wase, (1992). Effect of immobilization on the production of -amylase by an industrial strain of Bacillus amyloliquefaciens, J. Chem. Tech. Biotechnol, vol 53. :
  9. I. Chibata, (1978). Immobilized Enzymes Research and Development. New York : John Wiley and Sons
  10. I. Chibata, (1983). Immobili zed living microbial cells, Annu. Rev.Biophys. Bioeng.6. :
  11. T. Tosa, (1983). Immobili zed living microbial cells, Annu. Rev.Biophys. Bioeng.6. :
  12. M. Fujimura, (1983). Immobili zed living microbial cells, Annu. Rev.Biophys. Bioeng.6. :
  13. I. Chibata, (1986). Methods of cell immobilization dalam Manual of Industrial Microbilogy and Biotechnology. Washington DC : American Sociaty for Microbiology
  14. T. Tosa, (1986). Methods of cell immobilization dalam Manual of Industrial Microbilogy and Biotechnology. Washington DC : American Sociaty for Microbiology
  15. T. Sato, (1986). Methods of cell immobilization dalam Manual of Industrial Microbilogy and Biotechnology. Washington DC : American Sociaty for Microbiology
  16. I. Chibata, (1974). Use of immobilized cell system to prepare fine chemicals dalam Microbial Technology, II, 2nd ed.. : Academic Press
  17. T. Tosa, (1974). Use of immobilized cell system to prepare fine chemicals dalam Microbial Technology, II, 2nd ed.. : Academic Press
  18. T. Sato, (1974). Use of immobilized cell system to prepare fine chemicals dalam Microbial Technology, II, 2nd ed.. : Academic Press
  19. I. Chibata, (1974). Immobilized aspartase containing microbial cells : Preparation an Enzymatic Properties, Appl. Microbiol.27. :
  20. I. Chibata, (1974). Immobilized aspartase containing microbial cells : Preparation an Enzymatic Properties, Appl. Microbiol.27. :
  21. T. Tosa, (1974). Immobilized aspartase containing microbial cells : Preparation an Enzymatic Properties, Appl. Microbiol.27. :
  22. T. Sato, (1974). Immobilized aspartase containing microbial cells : Preparation an Enzymatic Properties, Appl. Microbiol.27. :
  23. P.M. Doran, (1986). Effects of immobilization on growth fermentation properties and macromolecular composition of Saccaharomyces cerevisiae, attached to gellatin, Biotech.Bioeng.,28. :
  24. J.E. Bailey, (1986). Effects of immobilization on growth fermentation properties and macromolecular composition of Saccaharomyces cerevisiae, attached to gellatin, Biotech.Bioeng.,28. :
  25. S.A. El Aassar, (1990). The biosynthesis of protease with fibrinolytic activity in immobilized culture of Penicillium chrysogenum H9, Appl. Microbiol, Biotechnol.,33. :
  26. H.M. El Badry, (1990). The biosynthesis of protease with fibrinolytic activity in immobilized culture of Penicillium chrysogenum H9, Appl. Microbiol, Biotechnol.,33. :
  27. A.F. Abdel Fatah, (1990). The biosynthesis of protease with fibrinolytic activity in immobilized culture of Penicillium chrysogenum H9, Appl. Microbiol, Biotechnol.,33. :
  28. A. Egresi, (0000). An Improved method for the entraptment of microbial cells into a thermogel, Biotechnol. Tech.,6,(3). :
  29. C.S. Siasak, (0000). An Improved method for the entraptment of microbial cells into a thermogel, Biotechnol. Tech.,6,(3). :
  30. I. Harsanyl, (0000). An Improved method for the entraptment of microbial cells into a thermogel, Biotechnol. Tech.,6,(3). :
  31. B. Szajani, (0000). An Improved method for the entraptment of microbial cells into a thermogel, Biotechnol. Tech.,6,(3). :
  32. A. Freeman, (1981). Immobilization of micobial cells in crosslinked, prepolimerized, linier poliacrilamid gel : antibiotic production by immobilized Streptomyces clavurigerus, Biotechnol. Bioeng.23 . :
  33. Y. Aharonowitz, (1981). Immobilization of micobial cells in crosslinked, prepolimerized, linier poliacrilamid gel : antibiotic production by immobilized Streptomyces clavurigerus, Biotechnol. Bioeng.23 . :
  34. E.M. Frein, (0000). Cellulase production by Trychoderma resei immobilezed on κ-carrageenan, Biotechnol. Lett.,4. :
  35. B.S. Montenecourt, (0000). Cellulase production by Trychoderma resei immobilezed on κ-carrageenan, Biotechnol. Lett.,4. :
  36. D.E. Eveleigh, (0000). Cellulase production by Trychoderma resei immobilezed on κ-carrageenan, Biotechnol. Lett.,4. :
  37. M. Eveleigh, (1984). Continous production of l-arginine using immobilized growing Serratia marcescens cells : effectiveness suply of oxygen gas, Appl. Microbiol. Bitechnol,19 . :
  38. J. Kato, (1984). Continous production of l-arginine using immobilized growing Serratia marcescens cells : effectiveness suply of oxygen gas, Appl. Microbiol. Bitechnol,19 . :
  39. T. Tosa, (1984). Continous production of l-arginine using immobilized growing Serratia marcescens cells : effectiveness suply of oxygen gas, Appl. Microbiol. Bitechnol,19 . :
  40. I. Chibata, (1984). Continous production of l-arginine using immobilized growing Serratia marcescens cells : effectiveness suply of oxygen gas, Appl. Microbiol. Bitechnol,19 . :
  41. L.A. Golovieva, (1993). Degradation of 2,4,6-TCP and a mixture of isomeric chlorophenols by immobilized Streptomyces rochei 303, Appl. Microbiol. Biotechnol.,38,(6). :
  42. O.E. Zaborina, (1993). Degradation of 2,4,6-TCP and a mixture of isomeric chlorophenols by immobilized Streptomyces rochei 303, Appl. Microbiol. Biotechnol.,38,(6). :
  43. A.Y. Arinbasarova, (1993). Degradation of 2,4,6-TCP and a mixture of isomeric chlorophenols by immobilized Streptomyces rochei 303, Appl. Microbiol. Biotechnol.,38,(6). :
  44. A. Groboillot, (1994). Immobilization of cells for application in the food industry, Critical Rev. In Biotechnol.,14,(2). :
  45. D.K. Boadi, (1994). Immobilization of cells for application in the food industry, Critical Rev. In Biotechnol.,14,(2). :
  46. D. Poncelet, (1994). Immobilization of cells for application in the food industry, Critical Rev. In Biotechnol.,14,(2). :
  47. R.J. Neufeld, (1994). Immobilization of cells for application in the food industry, Critical Rev. In Biotechnol.,14,(2). :
  48. D. Guoqiang, (1992). Immo bilazation of Lactobacillus casei cells to ceramics material pretreated with polyethylenimin, Appl. Microbil. Biotechnol.,37. :
  49. R. Kaul, (1992). Immo bilazation of Lactobacillus casei cells to ceramics material pretreated with polyethylenimin, Appl. Microbil. Biotechnol.,37. :
  50. B. Mattiasson, (1992). Immo bilazation of Lactobacillus casei cells to ceramics material pretreated with polyethylenimin, Appl. Microbil. Biotechnol.,37. :
  51. D.V. Klaus, (1973). The Proteolitic enzymes of K-1 starin of Streptomyces griseus obtained from commercial preparation (Pronase), J. Biol. Chem.,248,(17). :
  52. C. Kai-Fu, (1973). The Proteolitic enzymes of K-1 starin of Streptomyces griseus obtained from commercial preparation (Pronase), J. Biol. Chem.,248,(17). :
  53. M.A. William, (1973). The Proteolitic enzymes of K-1 starin of Streptomyces griseus obtained from commercial preparation (Pronase), J. Biol. Chem.,248,(17). :
  54. J. Klein, (1985). Immobilzation techniques cells, dalam Comprehensive Biotechno logy, editor by Moo Young, Humprey, A.E., Cooney, C.L.,2. :
  55. K.P. Vorlop, (1985). Immobilzation techniques cells, dalam Comprehensive Biotechno logy, editor by Moo Young, Humprey, A.E., Cooney, C.L.,2. :
  56. T. Kokubu, (1978). -amilase production by immobilized whole cells of Bacillus subtilis, Eur. J. Appl. Microbiol. Biotechnol.5. :
  57. I. Karube, (1978). -amilase production by immobilized whole cells of Bacillus subtilis, Eur. J. Appl. Microbiol. Biotechnol.5. :
  58. S. Suzuki, (1978). -amilase production by immobilized whole cells of Bacillus subtilis, Eur. J. Appl. Microbiol. Biotechnol.5. :
  59. B. Mattiasson, (1983). Immobilized Cells And Organeles. Cleveland : Chemical Rubber Co.
  60. K. Mosbach, (1966). Entrapment of enzyme and microorganism in synthetic crosslinked polymer and their application in coloumn techniques, Acta. Chem. Scand.,20. :
  61. R. Mosbach, (1966). Entrapment of enzyme and microorganism in synthetic crosslinked polymer and their application in coloumn techniques, Acta. Chem. Scand.,20. :
  62. C.F. Phil, (1988). Gel immobilized cell culture, Inter. Biteoch. Labor.,6,(2). :
  63. D.D. Nicoll, (1988). Gel immobilized cell culture, Inter. Biteoch. Labor.,6,(2). :
  64. G. Sarath, (1989). Protease Assay Methods, dalam Proteolytic Enzymes, a Pratical Approach, Beynon, R.J. and Bond, J.S., Ed.. New York : IRL Press
  65. S.M. Rebecca, (1989). Protease Assay Methods, dalam Proteolytic Enzymes, a Pratical Approach, Beynon, R.J. and Bond, J.S., Ed.. New York : IRL Press
  66. F.W. Wagner, (1989). Protease Assay Methods, dalam Proteolytic Enzymes, a Pratical Approach, Beynon, R.J. and Bond, J.S., Ed.. New York : IRL Press
  67. T. Sato, (1979). Immobilization of Escherechia coli cells containing aspartase activity with κ -carrageenan, Biochem. Biophys. Acta,570. :
  68. Y. Nishida, (1979). Immobilization of Escherechia coli cells containing aspartase activity with κ -carrageenan, Biochem. Biophys. Acta,570. :
  69. T. Tosa, (1979). Immobilization of Escherechia coli cells containing aspartase activity with κ -carrageenan, Biochem. Biophys. Acta,570. :
  70. S.S. Sidhu, (1994). Streptomyces griseus protease C - A novel of chimotrypsin superfamily,J.Biol.Chem.,269,(31). :
  71. G.B. Kalmar, (1994). Streptomyces griseus protease C - A novel of chimotrypsin superfamily,J.Biol.Chem.,269,(31). :
  72. L.G. Willis, (1994). Streptomyces griseus protease C - A novel of chimotrypsin superfamily,J.Biol.Chem.,269,(31). :
  73. T.J. Borgford, (1994). Streptomyces griseus protease C - A novel of chimotrypsin superfamily,J.Biol.Chem.,269,(31). :
  74. I. Takata, (1980). Immobilization of Brevibacterium flavum with carrageenan and its application for continous production of L-malic acid , Enzyme Microb. Technol.,60. :
  75. K. Yamamoto, (1980). Immobilization of Brevibacterium flavum with carrageenan and its application for continous production of L-malic acid , Enzyme Microb. Technol.,60. :
  76. T. Tosa, (1980). Immobilization of Brevibacterium flavum with carrageenan and its application for continous production of L-malic acid , Enzyme Microb. Technol.,60. :
  77. I. Chibata, (1980). Immobilization of Brevibacterium flavum with carrageenan and its application for continous production of L-malic acid , Enzyme Microb. Technol.,60. :
  78. T. Tosa, (1979). Immobilization of enzymes and microbial cells using carrageenan as matrix. Biotechnol. Bioeng.,21. :
  79. T. Sato, (1979). Immobilization of enzymes and microbial cells using carrageenan as matrix. Biotechnol. Bioeng.,21. :
  80. T. Mori, (1979). Immobilization of enzymes and microbial cells using carrageenan as matrix. Biotechnol. Bioeng.,21. :
  81. K. Yamamoto, (1979). Immobilization of enzymes and microbial cells using carrageenan as matrix. Biotechnol. Bioeng.,21. :
  82. I. Takata, (1979). Immobilization of enzymes and microbial cells using carrageenan as matrix. Biotechnol. Bioeng.,21. :
  83. Y. Nishida, (1979). Immobilization of enzymes and microbial cells using carrageenan as matrix. Biotechnol. Bioeng.,21. :
  84. I. Chibata, (1979). Immobilization of enzymes and microbial cells using carrageenan as matrix. Biotechnol. Bioeng.,21. :
  85. W.R. Vieth, (1979). Immobilized cells in complexs biocatalysis dalam Immobilized Microbial Cells, Venkatasubramanian, K. Ed., ACS Symposium Series. Washington DC : American Chemical Society
  86. K. Venkatasubramanian, (1979). Immobilized cells in complexs biocatalysis dalam Immobilized Microbial Cells, Venkatasubramanian, K. Ed., ACS Symposium Series. Washington DC : American Chemical Society
  87. J.C. Vuillemard, (1988). Continues production of small peptides from milk protein by extracellular proteases of free and immobilized Serratia marces cens cells, Enzyme Microbiol. Technol.,10. :
  88. J. Goulet, (1988). Continues production of small peptides from milk protein by extracellular proteases of free and immobilized Serratia marces cens cells, Enzyme Microbiol. Technol.,10. :
  89. J. Amiot, (1988). Continues production of small peptides from milk protein by extracellular proteases of free and immobilized Serratia marces cens cells, Enzyme Microbiol. Technol.,10. :
  90. M.A. Vijayalakshmi, (1988). Continues production of small peptides from milk protein by extracellular proteases of free and immobilized Serratia marces cens cells, Enzyme Microbiol. Technol.,10. :
  91. S. Terre, (1988). Continues production of small peptides from milk protein by extracellular proteases of free and immobilized Serratia marces cens cells, Enzyme Microbiol. Technol.,10. :
  92. J.C. Vuillemard, (1988). Protease production by immobilized growing cells of Serratia marcescens and Myxococcus xanthus in calsium alginat gel beads, Appl. Microbiol. Biotechnol.,27. :
  93. S. Terre, (1988). Protease production by immobilized growing cells of Serratia marcescens and Myxococcus xanthus in calsium alginat gel beads, Appl. Microbiol. Biotechnol.,27. :
  94. S. Benoit, (1988). Protease production by immobilized growing cells of Serratia marcescens and Myxococcus xanthus in calsium alginat gel beads, Appl. Microbiol. Biotechnol.,27. :
  95. J. Amiot, (1988). Protease production by immobilized growing cells of Serratia marcescens and Myxococcus xanthus in calsium alginat gel beads, Appl. Microbiol. Biotechnol.,27. :
  96. A.S. Zakran, (1992). Production of Protease by immobilized and free cells of Flavobacterium sp. R23, Milchwiss,48. :
  97. G. Zayed, (1992). Production of Protease by immobilized and free cells of Flavobacterium sp. R23, Milchwiss,48. :
  98. Tanabe Seiyaku Co., (1976). Immobilized Cell, Annual Reports on Fermentation Processes, , Perlman dan Tsao Ed.,2. : Tanabe Seiyaku Co.


   


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