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Majalah Farmasi Airlangga (Airlangga Journal of Pharmacy)

ISSN 0852-1050

Vol. 6 / No. 1 / Published : 2008-04

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Original Article :

The comparison of densitometry and hplc method for determination of codein phosphate and paracetamol simultaneously in analgesic tablet

Author :

      Abstract :

      Combination between codein and paracetamol was used as symptomatic relief of severe pain in palliative cancer therapy. Composition of codein and paracetamol in tablets were 20:500 to 40:500. Problems of quantitative analysis of those combinations were the higher ratio concentration of codein and paracetamol, the weaker sensitivity of codein in compare with paracetamol and the overlapping of their spectra’s profile. The simple and cheaper TLC-Densitometry was proposed to solve those problems. Result of TLC-Densitometry for determination of codein and paracetamol simultaneously was verified with the official determination of those compounds simultaneously by HPLC. The aim of this study was to compare the result of TLC-Densitometry and HPLC methods for determination of codein and paracetamol simultaneously in tablets. Determination of codein and paracetamol by TLC-Densitometry using a mixture of methanol and ammonia (33:0.5) as eluent shows good resolution (Rs>2). The codein and paracetamol recoveries were (92.29 + 7.46)% and (103.20 + 6.42)% respectively. Whereas,  determination of codein and paracetamol by HPLC method using a mixture of methanol and phosphate buffer 20 mM pH 2.5 (15:85) as eluent, zorbax C8 as column and detected by DAD (l=284 nm) shows better recoveries of codein and paracetamol  i.e (100.50+0.75)% and (99.99+0.64)% respectively. Linearity of codein or paracetamol using TLC-Densitometry was as good as linearity of codein or paracetamol using HPLC. Determination of codein and paracetamol by TLC-Densitometry was not yet as good as the results of determination of codein and paracetamol by HPLC method. But, TLC-densitometry is still useful and important as alternative method (e.g. in the absence of HPLC method) because TLC-densitometry was relatively rapid, simple and cheaper than HPLC.

      Keyword :

      Codein, Paracetamol, TLC-Densitometry, HPLC,


      References :



           


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