UNIVERSITAS AIRLANGGA



Detail Article

Majalah Ilmu Faal Indonesia

ISSN 0215-1995

Vol. 10 / No. 2 / Published : 2013-09

Order : 9, and page :113 - 122

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Original Article :

The effect of catechins green tea toward p-110 phosphatidil inositol 3- kinase ( pi3k ) expression and p38 mitogen activated protein kinase (mapk ) activity in aortic male rats induced high fat diet

Author :

  1. Erna Susanti*1
  2. Achmad Rudijanto*2
  3. Retty Ratnawati*3
  1. Akademi Farmasi Putra Indonesia Malang
  2. Divisi Endokrin dan Metabolik IPD RS Saiful Anwar Malang/ Fakultas Kedokteran Universitas Brawijaya
  3. Lab. Ilmu Faal Divisi Fisiologi Molekuler Fakultas Kedokteran Universitas Brawijaya Jalan Veteran Malang

Abstract :

Catechins, isolate from green tea  GMB4 clones could be developed as a potential preventive agent for atherosclerosis. This activity is thought to be based on the structure epimerisation, hydroxy phenolic  and galloil group of these isolates. Therefore the purpose of this study is to prove whether catechins ,isolate from green tea,  inhibits the decrease of p110-PI3K expression and  the increase of p38-MAPK activity in male wistar rats induced by  high  fat diet. The method used in this study were Western Blott and ELISA to determine  p110-PI3K expression and  activity of p38 MAPK, respectively. The results showed that  catechins inhibited significantly (p<0.05) the decrease of the expression of p110 PI3K at dose of 6 mg/day and 24 mg/day,but not in the dose 3mg/day. This suggested that dose 6mg/day and 24 mg/day was able to repair the damage signaling insulin on PI3K pathway caused by insulin resistance. While the dose  3 mg/day of catechins  inhibited the increase activity of p38 MAPK. These could be  based on the ability of catechins as antioxidant. Catechins with hydroxy phenolic group could be  a potential electron donor to bind  the free radicals. Polyphenol catechins may also modulate  signaling cellular processes, such as NFkβ, activator protein-1 DNA binding, glutathione biosynthesis, PI3K/Akt, MAPK pathway and the Nrf2 transcription factor that regulates expression of several antioxidant genes. It can be concluded that catechins  inhibits  the decrease of p110 PI3K  (at  dose of 6 mg/day and 24 mg/day) and also the increase of  p38 MAPK activity (in all treatment doses).

Keyword :

catechins, p110-PI3K, p38 MAPK activity, aorta, male wistar rats,


References :

Ali M, Muliartha IK,(2004) Optimasi Diet Tinggi Lemak pada Tikus Model Atherogenik Malang : Jurnal Kedokteran Brawijaya. 3(2) : 15-21





Archive Article

Cover Media Content

Volume : 10 / No. : 2 / Pub. : 2013-09
  1. The Effect Of Preparation Period Training Program Towards Liver Function On The Athelets Of Pon Xviii 2012 Potential Contingent Of Koni South Sulawesi
  2. The Role Of Vitamin E To Stimulate Secretion Of Endogenic Antioxidant By Increasing Of Blood Glutation Level Secretion To The Marathon Athletes 3000 Meters Run Pelatda Pon Xviii Aceh Provinces
  3. Erytrocyte, Haemoglobin, And Hematocrit Of Java Common Palm Civets (paradoxurus Hemaphroditus)
  4. Effect Of Ethanolic Extract Of Neem (azadirachta Indica A.juss) Leave And Seed To The Female Mice Reproduction (mus Musculus Albinus)
  5. Body Weight As The Modulator Of Oxidative Stress During Aerobik Exercise For A Momment
  6. Profile Of Partial Volatile Fatty Acids(vfa) Of Sheep Induced By Fraction Katuk Leaf Ekstract (sauropus Androgynus L. Merr).
  7. The Antioxidant Activities Of Honey, Olive Oil And Nigela Sativa Oil On The Rats Model Of Dyslipidemia
  8. The Effect Of Rosella Red Tea Steeping (hibiscus Sabdariffa L) On Fasting Glucose Level, Two Hours Post Prandial And Blood Triglycerides
  9. The Effect Of Catechins Green Tea Toward P-110 Phosphatidil Inositol 3- Kinase ( Pi3k ) Expression And P38 Mitogen Activated Protein Kinase (mapk ) Activity In Aortic Male Rats Induced High Fat Diet
  10. The Effect Of Rosella Tea (hibiscus Sabdariffa) On The Mice Which Are Treated By Alcohol To The Prevention Of Hypertension

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