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Media Kedokteran Hewan

ISSN 2015-8930

Vol. 21 / No. 2 / Published : 2005-05

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Original Article :

Antibody response and phagocytosis activity of peritoneal macrophage in mice fed pasteurized and fermented horse milk protein

Author :

  1. Nurliyani*1
  2. Wayan Tunas Artama*2
  3. Zuheid Noor*3
  1. Department of Animal Product Technology, Faculty of Animal Husbandry, Gadjah Mada University
  2. Laboratory of Biochemistry, Faculty of Veterinary Medicine, Gadjah Mada University
  3. Laboratory of Food and Nutrition, Faculty of Agriculture Technology, Gadjah Mada University

Abstract :

Many kinds of milk protein components have different chemical and physical characteristics that determine its bioactive properties, including its immune function. The aim of this research were to isolate and characterize of protein in pasteurized and fermented horse milk as immunomodulator in mice humoral (serum antibody) and cellular immune responses (lymphocyte proliferation and phagocytosis activity). Protein of pasteurized horse milk was fractionated on Sephadex G-75 column, and obtained two protein fractions (fraction 1 and fraction 2). Female mice BALB/c 6 – 8 weeks old were administered orally with protein of pasteurized and fermented horse milk up to 28 day of experiment. On the 7th, 14th, 21th, and 28th day of experiment, the serum IgG and IgA were analized with enzyme-linked immunosorbent assay (ELISA). Whereas phagocytosis activity of peritoneal macrophage and lymphocyte proliferation were analyzed on the 14th and 28th day of experiment. The pasteurized and fermented horse milk protein were characterize with sodium dodecyl sulphate polyacrylamide gel electrrophoresis (SDS-PAGE). The result of this research showed that protein of pasteurized and fermented horse milk can take part as immunomodulator in humoral immune response, stimulate in phagocytosis activity, but there were no effect on lymphocyte proliferation.

Keyword :

Antibody, phagocytosis, horse milk protein, pasteurized, fermented,


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