Media Kedokteran Hewan
ISSN 2015-8930
Vol. 25 / No. 2 / Published : 2009-05
Order : 14, and page :82 - 89
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Original Article :
Characterization and the increase of chicken interferon-gamma production as a measure of t-cell responses to eimeria tenella antigens
Author :
- Yunus M*1
- Suwarno*2
- Suwanti LT*3
- Mufasirin*4
- Dosen Fakultas Kedokteran Hewan
- Fakultas Farmasi
- Dosen Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
Abstract :
Characterization and the increase of chicken interferon-gamma (ChIFN_Ƴ) production were investigated in Eimeria tenella (E.tenella) infected chicken. Twenty poultry broilers at three weeks old were divided into two groups. The first group was control and those chickens were not infected anything. The second group was infected with 4 x 103 of E.tenella. Spleen cells of both the groups were cultured and induced mitogen. This study was to characterize ChIFN_Ƴ protein through extraction technique of supernatant protein of the culture medium of chicken spleen cells that was induced Con-A by SDS-PAGE. The result of the characterization showed that molecule weight of ChIFN_Ƴ protein was 17 kDa. Then, Optical Density (OD) of ChIFN_Ƴ protein was detected ELISA reader 450 nm of both E.tenella uninfected and infected chickens was 1.29 and 1.95, respectively, whereas, concentration of ChIFN_Ƴ protein of both those groups was 10.312 pg/ml and 15.569 pg/ml, respectively
Keyword :
E.tenella, chicken interefon-gamma,
References :
Byrnes S, Eaton R, and Kogut M,(1993) In vitro interleukin-1 and tumor necrosis factor-alpha production by macrophages from chickens infected with either Eimeria maxima or Eimeria tenella - : Int. J. Parasitol. 23:639-645
Choi KD, Lillehoj HS, and Zalenga DS,(1999) Changes in local IFN-Ƴ and TGF-β4 mRNA expression and intraepithelial lymphocytes following E. acervulina infection - : Vet. Immuniol. Immunopathol. 71:263-275
Dijkmans R, and Billiau A,(1988) Interferon gamma: a master key in the immune system - : Curr. Opin. Immunol. 1:269-274
Houglum JE,(1983) Interferon: mechanism of action and clinical value - : Clin. Pharm. 2:20-28
Hughes HPA, Speer CA, Kyle JE, and Dubey JP,(1987) Activation of murine macrophages and a bovine monocyte cell line by bovine lymphokines to kill the intracelluler pathogens Eimeria bovis and Toxoplasma gondii - : Infect. Immun. 55:784-791
Jenkins MC, Castle MD, and Danforth HD,(1991) Protective immunization against the int
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