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Media Kedokteran Hewan

ISSN 2015-8930

Vol. 22 / No. 2 / Published : 2006-05

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Original Article :

Characterization of avian influenza virus by serological test and reverse transcriptase-polymerase chain reaction

Author :

  1. Suwarno*1
  2. Adi Prijo Rahardjo*2
  3. Fauziah*3
  4. Eko Agus Srihanto*4
  1. Bagian Mikrobiologi, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya
  2. Bagian Mikrobiologi, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya
  3. Balai Penelitian dan Pengujian Veteriner Regional III, Tanjung Karang
  4. Balai Penelitian dan Pengujian Veteriner Regional III, Tanjung Karang

Abstract :

The aims of this study were identified of avian influenza (AI) virus by serological test, and characterized of cDNA virus by reverse transcriptase-polymerase chain reaction (RT-PCR). The samples were taken from live or dead birds that include cloacal and tracheal swab, and internal organs (lungs, spleen, pancreas and kidney). The suspensions in antibiotic solution of cloacal and tracheal swab were inoculated into the allantoic cavity of 10-day-old embryonated fowl eggs. The allantoic fluid of embryonated eggs containing AI virus identified by hemagglutination inhibition (HI) and immunodiffusion (ID) test. The presence of AI virus from allantoic fluid or internal organs could confirmed by the use of RT-PCR with H5-specific primers. The result showed that HI test could used by the identification of AI virus from allantoic fluid or the internal organ, but ID test was detected samples in allantoic fluid only. The PCR product was amplificated in this technique was 235 base pairs (bp) against to hemagglutinin gene and amplicon with 200-300 bp against to matrix gene, and specifically for the presence of H5 subtype.

Keyword :

avian influenza, HI test, ID test, RT-PCR,


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