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Media Kedokteran Hewan

ISSN 2015-8930

Vol. 30 / No. 1 / Published : 2014-01

TOC : 1, and page :1 - 8

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Original Article :

Correlation between motility with viability and membrane integrity of boer goat sperm before freezing and post thawing

Author :

  1. Nurul Isnaini*1
  1. Staf Pengajar Fakultas Peternakan Unibraw

Abstract :

Abstrak      Penelitian ini dilakukan untuk mengetahui korelasi motilitas dengan viabilitas dan integritas membran sperma kambing Boer  before freezing dan post thawing . Semen ditampung dari 6 ekor kambing Boer dewasa. Setelah penampungan, semen segera dievaluasi kualitasnya. Pengenceran dilakukan dengan pengencer dasar tris dengan ratio 1 bagian semen dan 9 bagian pengencer.   Segera setelah pengenceran, semen disimpan pada suhu 3-5°C selama 3-4 jam dan diamati persentase motilitas, viabilitas dan integritas membran  sperma, setelah itu dilakukan pre freezing pada suhu -140° C di uap Nitrogen cair dan dilanjutkan dengan freezing dalam Nitrogen cair suhu -196° C. Setelah freezing 2 X 24 jam semen beku di thawing pada suhu 37 C selama 30 detik dan selanjutnya diamati motilitas, viabilitas dan integritas membran. Hasil penelitian menunjukkan bahwa motilitas sperma memiliki korelasi positif sangat nyata dengan viabilitas dan integritas membran sperma sedangkan viabilitas memiliki korelasi positif nyata terhadap integritas membran before freezing; demikian juga motilitas memiliki korelasi positif sangat nyata terhadap viabilitas maupun integritas membran dan viabilitas juga memiliki korelasi positif sangat nyata terhadap integritas membran  post thawing.   Dari hasil penelitian dapat disimpulkan bahwa motilitas sperma memiliki korelasi yang sangat erat dengan viabilitas dan integritas membran sperma, baik pada saat before freezing maupun post thawing, sedangkan viabilitas sperma memiliki korelasi yang sangat erat pada saat before freezing dan erat pada saat post thawing  dengan persentase  integritas membran sperma kambing Boer.  Untuk mengetahui kualitas semen dengan praktis, bisa dilakukan hanya mengamati motilitas individu sperma saja, karena motilitas individu memiliki korelasi yang erat dengan variabel sperma yang lain.   Abstract      This study was conducted to evaluate the correlation between   motility with viability and membrane integrity of Boer goat sperm before freezing and post thawing. Semen was collected from 6 male mature Boar goats. Semen was diluted with tris-based medium   with the ratio of 1 semen : 9 diluter. Immediately after dilution semen was stored in  3-5°C  for 3-4 hours, and sperm motility, viability and membrane integrity   percentage  were observed. Next semen was stored at -140°C for 10 minutes and plunged in liquid nitrogen at -196°C for 2 X 24 hours, semen be thawed and sperm motility, viability and membrane integrity   percentage  were observed. The result showed that sperm motility  have very significant positive correlation with sperm membrane integrity, but viability have   significant positif correlation with sperm membrane integrity percentage of Boer goat semen before freezing. While sperm motility and viability have very significant positif correlation with sperm membrane integrity post thawing. It was concluded, that sperm motility have very closed correlation with sperm membrane integrity, but viability have closed correlation with sperm membrane integrity before freezing; while both motility and viability have very closed correlation with sperm membrane integrity post thawing. For practice,  only evaluation of sperm motility is enough to know semen quality, because sperm motility have closed correlation with other sperm variable.

Keyword :

correlation, motility, viability, membrane integrity, before freezing and post thawing,


References :

  1. Anonimus, (2006). Petunjuk Penampungan, Produksi, Distribusi dan Evaluasi Semen Beku. Balai Inseminasi Buatan. Singosari. Malang : -
  2. Isnaini, N, (2006). Peranan Trehalosa dalam Pendinginan dan Pembekuan Semen Kambing Boer. Disertasi. Progrm Pascasarjana Universitas Brawijaya. Malang : -
  3. Isnaini, N, (2008). Korelasi Motilitas dengan Viabilitas dan Integritas Membran Sperma Kambing Boer Selama Penyimpanan Dingin. J. Dinamika Rekasatwa. Vol. 3 No.1. Hal: 1-10.. - : -
  4. Van Niekrek, W.A. and NH. Cassey, (1988). The Boergoet.Growth, Nutrient Requirements, Carcass and Meat Quality. Department of Livestock Science; Faculty of Agriculture, University of Pretoria. South Africa. South Africa : -
  5. Widodo, M. A, (2003). Calsium dan Generasi Spesies Oksigen Reaktif pada Fungsi Mitokondria. Buku Kumpulan Makalah. Basic Molecular Biology Course on Mitochondrial Medicine. Fakultas Kedokteran Unibraw. Malang. Malang : -


   


Archive Article

Cover Media Content

Volume : 30 / No. : 1 / Pub. : 2014-01
  1. Correlation between motility with viability and membrane integrity of boer goat sperm before freezing and post thawing
  2. Peningkatan kualitas semen beku kambing pe melalui suplementasi antioksidan α–tocoferol dalam pengencer semen untuk inseminasi buatan
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