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Media Kedokteran Hewan

ISSN 2015-8930

Vol. 22 / No. 3 / Published : 2006-09

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Original Article :

Detection of jembrana disease viral antigen in peripheral blood lymphocytes by monoclonal antibodies

Author :

  1. Nyoman Mantik Astawa*1
  2. Nining Hartaningsih*2
  3. Luh Putu Agustini*3
  4. Wayan Masa Tenaya*4
  5. Ketut Berata*5
  6. Luh Putu Manik Widiyanti*6
  1. Fakultas Kedokteran Hewan Universitas Udayana, Jln. PB Sudirman, Denpasar 80232, Tel. 0361223791,
  2. Laboratorium Bioteknologi, Balai Penyidikan dan Pengujian Veteriner Wilayah VI Denpasar, Bali
  3. Laboratorium Bioteknologi, Balai Penyidikan dan Pengujian Veteriner Wilayah VI Denpasar, Bali
  4. Laboratorium Bioteknologi, Balai Penyidikan dan Pengujian Veteriner Wilayah VI Denpasar, Bali
  5. Fakultas Kedokteran Hewan Universitas Udayana, Jln. PB Sudirman, Denpasar 80232, Tel. 0361223791
  6. Universitas Pendidikan Ganesha, Singaraja Bali

Abstract :

The antigen of Jembrana disease virus (JDV) was detected in peripheral blood lymphocytes of Bali cattle by monoclonal antibodies against the capsid protein of the virus. The monoclonal antibodies were produced by fusion of myeloma cells with lymphocytes of mice immunized with JDV antigen. They were then used to detect JDV antigen in peripheral blood lymphocytes. JDV antigen was detected by immunositochemistry technique as early as day-3 post-infection with the percentage of infected cells of 0,74%. The percentage of infected cells increased substantially at day-7 post-infection (6,21%), and started to decline at day-9 (5,12%) and day-11 (3,87%) post-infection. A similar result was obtained by western blotting, with which JDV antigen was detectable as early as day-4 post-infection with a very weak “band”. becoming more evident at day-5, 7, and 9 post-infection. At day-11 post-infection, a weaker band was still detectable. The detection of JDV antigen in peripheral blood lymphocytes was accompanied by the rise of body temperature and the decline of total leucocyte counts. A rapid and an accurate diagnosis of JDV infection in Bali cattle can therefore be established by the detection of JDV antigen in peripheral blood lymphocytes, determination of body temperature and total blood leucocyte count.

Keyword :

Monoclonal antibodies, capsid protein, lymphocytes, Bali cattle, peripheral blood,


References :

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