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Media Kedokteran Hewan

ISSN 2015-8930

Vol. 22 / No. 1 / Published : 2006-01

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Original Article :

Estrous cycle and serum progesterone level of mice (mus musculus) before and after immunization using antifertility substance goat zona pellucida-3 (gzp3

Author :

  1. Sri Mulyati*1
  2. Imam Mustofa*2
  3. Mahaputra*3
  1. Department of Reproduction and Obstetric, Faculty of Veterinary Medicine, Airlangga University.
  2. Department of Reproduction and Obstetric, Faculty of Veterinary Medicine, Airlangga University.
  3. Department of Reproduction and Obstetric, Faculty of Veterinary Medicine, Airlangga University.

Abstract :

The purpose of this study was to determine the possibilities change of estrous cycle and serum progesterone profile of mice (Mus musculus) immunized with fraction-3 of goat zona pellucida (gZP3) as an anti fertility substance. The first immunization, gZP3 suspension was mixed with Complete Freund’s Adjuvant (CFA) 1:1 (v/v) followed by boosters twice with the interval of 11 days, the suspension was mixed with Incomplete Freund’s Adjuvant (IFA) 1:1 (v/v). Oestrous cycles were observed by means of vaginal smear evaluation before each bleed. Blood samples collection to got serum were carried out four times, before first immunization, before first booster, before second booster and after second booster. The measurement of progesterone mice serum using radioimmunoassay technique. The results showed that estrous cycle and progesterone serum level of the first immunization until to the second booster injection were in conformity with follicular and luteal phase respectively. It concluded that immunization by using of gZP3 as an anti fertility substance was not altered the oestrous cycle of mice and the level of progesterone level based on its estrous cycle.

Keyword :

goat zona pellucida-3, oestrous cycles, progesterone. ,

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