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Media Kedokteran Hewan

ISSN 2015-8930

Vol. 24 / No. 1 / Published : 2008-01

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Original Article :

Identification of avian reovirus in chickens with tenosynovitis using indirect sandwich-elisa and protein characterization by western blot

Author :

  1. Adi Prijo Rahardjo*1
  2. Nanik Sianita Widjaja*2
  3. Suwarno*3
  4. Jola Rahmahani*4
  5. Rahaju Ernawati*5
  6. Fedik A. Rantam*6
  1. Departemen Mikrobiologi Veteriner Fakultas Kedokteran Hewan ,Universitas Airlangga
    Jl. Mulyorejo Surabaya-60115. Tlp. 031-5992785.
  2. Departemen Mikrobiologi Veteriner Fakultas Kedokteran Hewan ,Universitas Airlangga
    Jl. Mulyorejo Surabaya-60115. Tlp. 031-5992785.

  3. Departemen Mikrobiologi Veteriner Fakultas Kedokteran Hewan ,Universitas Airlangga
    Jl. Mulyorejo Surabaya-60115. Tlp. 031-5992785.

  4. Departemen Mikrobiologi Veteriner Fakultas Kedokteran Hewan ,Universitas Airlangga
    Jl. Mulyorejo Surabaya-60115. Tlp. 031-5992785.
  5. Departemen Mikrobiologi Veteriner Fakultas Kedokteran Hewan ,Universitas Airlangga
    Jl. Mulyorejo Surabaya-60115. Tlp. 031-5992785.
  6. Departemen Mikrobiologi Veteriner Fakultas Kedokteran Hewan ,Universitas Airlangga
    Jl. Mulyorejo Surabaya-60115. Tlp. 031-5992785.

Abstract :

The aims of this study were identified of avian reovirus (ARV) from the chickens with tenosynovitis by indirect sandwich-enzyme linked immunosorbent assay (is-ELISA), and analysis of protein virus by sodium dodecyl sulphate (SDS-PAGE), and characterized of protein by western blot. Several chickens from flock with the hock-joint disturbance used a sample in the study. Virus was isolated in chicken embryonated eggs (CEE) and chicken embryo fibroblast (CEF) from the lymph, respiratory tract (trachea), gastrointestinal tract (intestine), synovial fluid and hock-joint suspension of suspected chick with the AEV infection. The viral isolate was well adopted and titrated at second passage level in CEE and CEF. The is-ELISA revealed that the isolate is of reovirus. In western blot protein of ARV detected was antigenic and have molecular weight were 147,5; 130,6; 114,3; 74,1; 70,8; 41; 38,8 and 31,7 kDa.

Keyword :

Avian reovirus, hock-joint, antigenic protein, sandwich-ELISA, western blot ,


References :

  1. NM Astawa, (2006). Neutralization of avian reovirus 1091 in chick kidney and Vero cell cultures by mo¬noclonal antibodies against μB/μBC and σC pro¬teins. Surabaya : Media Kedokteran Hewan. 22(2)
  2. Suwarno, (2005). Identifikasi virus rabies yang diadap¬ta¬si pada kultur sel neuroblastoma dengan indirect sandwich-ELISA dan direct-FAT. Surabaya : Media Kedokteran Hewan. 21(1)


   


Archive Article

Cover Media Content

Volume : 24 / No. : 1 / Pub. : 2008-01
  1. Antigenecity, sensitivity and spesificity of 27-28 kda protein of fasciola spp excretory-secretory (es) material for distomatosis diagnosis in cattle sera by using indirect-elisa technique
  2. Detection of toxoplasma gondii on the local chicken eggs sold as the mixture of traditional medicine in surabaya using biological test
  3. Identification of avian reovirus in chickens with tenosynovitis using indirect sandwich-elisa and protein characterization by western blot
  4. The inhibition of vitamin e (α- tocopherol ) antioxidant to superoxide radical reactive oxygen species (o2.-) production on the white rat (rattus norvegicus)stressed by an electric shock
  5. Intravenous phosphate infusion results in hyperphosphatemia and hypocalcemia without changing in plasma parathyroid hormone and calcitriol concentrations in goats
  6. Ultrasonography Diagnostic For Detection Of The Uterus Disorders In Cats (felis Catus)
  7. Potency of mengkudu (morinda citrifolia) juice extraction on carcass quality, vitamin c level and malonedialdehide (mda) level in broiler’s blood
  8. Comparison of fertility of etawa crossbred goats in low versus ideal body condition score after estrus synchronization and artificial insemination
  9. Digestibility and consumption of dry matter and organic matter in haylage complete feed ongole crossbreed steers
  10. The increase of desidual macrophages activity in infected toxoplasma gondii pregnant mice
  11. Restricted feeding delayed sexual maturation, decrease biometry of reproductive organ and fat deposition of quail hens (coturnix coturnix japonica)
  12. Detection of toxoplasma gondii on the local chicken eggs sold as the mixture of traditional medicine in surabaya using biological test
  13. The increase of desidual macrophages activity in infected toxoplasma gondii pregnant mice