Catalog :

Detail Article

Media Kedokteran Hewan

ISSN 2015-8930

Vol. 27 / No. 2 / Published : 2011-05

TOC : 4, and page :95 - 99

Related with : Scholar   Yahoo!   Bing

Original Article :

Increased of type 1-collagen expression in vaginal wall of the ovariectomized rats (rattus novergicus) strain wistar after ovariectomy on centella asiatica extract

Author :

  1. Abkar Raden*1
  2. Widjiati*2
  1. Dosen Fakultas Kedokteran
  2. Dosen Fakultas Kedokteran Hewan

Abstract :

Many cells, particularly vaginal cells, become atrophied in menopausal women. This condition will result in an inhibition of the epithelial cell maturation and reduced collagen synthesis so that vaginal elasticity will also decrease. This condition can be improved by the administration of estrogen or phytoestrogen . Centella asiatica is one of the plants that contain phytoestrogens with several essential components such as asiaticoside, Asiatic acid and madecassic acid could increase synthesis collagen. The objective of this study was to investigate the thickening and increasing collagen of the vaginal wall of ovariectomized Wistar rats treated with Centella asiatica extract . Ovariectomized rats were divided into 4 treatment groups, where rats in P1 group was not   administered the extract of the Centella asiatica ; Meanwhile rats in P2, P3 and P4 wsa administered the Centella asiatica extract at dose of 30, 60 and 120 mg/kg BW respectively per   day for 40 days. On day 61, the rats were sacrificed and examined immunohistochemically for the assessment of their type-1 collagen expression.   In conclusion, the expression of the type-1 collagen producing cells increased significantly by the administration of Centella asiatica extracts at doses of 30 , 60 and 120 mg/ kg BW daily for 40 day.. However, the 120 mg one was the most optimal dose that was able to enhance type-1 collagen synthesis. This proved that Centella asiatica extract contained phytoestrogen , which could increase the thickening and elevate the collagen of vaginal walls of the ovariectomized rats.

Keyword :

Centella asiatica extract, vaginal wall, ovariectomy, type-1 collagen,

References :

  1. Al-Baghdadi O, Ewies AAA, (2009). Topical estrogen therapy in the management of post meniopausal vaginal atrophy : An up-to date overview. 12. 91-105 : J.Climacteric
  2. Ananingati, (2007). Pengaruh isoflavon genestein daidzein ekstrak Pueraria Lobbata terhadap ekspresi reseptor beta pada endotel aorta tikus hipoestrogen. - : -
  3. Anne Katz PHD RN, (2007). when sex hurts: Menopause related dyspareunia . - : America J Nursing
  4. Baziad Ali, (2003). Menopause dan Andropause. 100-123 : Jakarta: Yayasan Bina Pustaka Sarwono Prawirohardjo
  5. Bonte F, Dumas M, M Chaudagne C, Meybeck A, (1994). Influence of Asiatic acid, madecassic acid, and asiaticoside on human collagen 1 synthesis. 72:316-320 : Planta Med
  6. Coldren, Christopher D., Hashim, P., Ali, M. Johari, OH, Se-Kyung.,Sinkey anthony J., Rha Cho Kyun, (2003). Gene expression in the Human Fibroblast induced by Centella asiatica Triterpenoids. 725-732 : Planta Med New York
  7. Cuozi RJ, Helzsouer KJ, Fetting JH, (1995). Prevalence of menopausal symptom among women with a history of breast cancer and attitudes to wardestrogen replacement therapy. - : Johns hopkin:medical inst
  8. Dennerstein L, Dudley EC, Hopper JL, Guthrie JR, Burger HG, (2000). A Prospectivepopulation-based study of menopausal symptoms. 96: 351-8 : Obstet Gynecol
  9. Fadhilah, (2005). Seminar sehari dalam rangka peringatan hari ibu . - : -
  10. Gynecological, (2008). URL:2008. ecological.htm. accessed Jan 23, . - : -
  11. Lee Jongsung , (2006). Asiaticoside Induce Human Collagen I Synthesis through TGFβ Receptor I Kinase (TβRI Kinase)-Independent Smad Signaling. 72: 324-328 : Planta Med
  12. Maquart FX, Bellon G, Gillery P, wegrowski Y, Borel YP, (1990). Stimulation of collagensynthesis in fibroblast cuby triterpenes from Cetella asiaticastimulate extracellular matrix accumulation in rat experimental wounds. 9 (4): 289-96 : European J. dermatology.
  13. Samil RS, (1989). wanita menjelang usia maturitas. 7-8 : Bag. Obs dan Gine. FKUI Jakarta
  14. Shukla A, Rasik AM, Dhawan BN, (1999). Asiaticoside-induced elevation of a ntioxidant levels in healing wounds. 13: 50-4 : Phytoter Res.
  15. Velde CV, (2000). BNIP3 and Genetic Control of necrosis Like cell death through the mitochondrial Permeabelity Transition Pore. 20: 5464-5468 : J Molr and Cellr Biol-
  16. Winarto W.P, Surbakti M, (2005). Khasiat dan Manfaat Pegagan Tanaman Penambah Daya Ingat. 2-3 : Agro Media Pustaka. Jakarta
  17. Zainuddin M, (2000). Metodologi Penelitian; Universitas Airlangga Pasca sarjana. - : -


Archive Article

Cover Media Content

Volume : 27 / No. : 2 / Pub. : 2011-05
  1. Increased of type 1-collagen expression in vaginal wall of the ovariectomized rats (rattus novergicus) strain wistar after ovariectomy on centella asiatica extract