UNIVERSITAS AIRLANGGA



Detail Article

Media Kedokteran Hewan

ISSN 2015-8930

Vol. 21 / No. 3 / Published : 2005-09

Related with : Scholar   Yahoo!   Bing

Original Article :

Induction of apoptosis by andrographolide compound from sambiloto (andrographis paniculata nees) in cancer cells culture

Author :

      Abstract :

      The objective of this research was to evaluate the activity of andrographolide extracted from sambiloto (Andrographis paniculata Nees) in induction of apoptosis in HeLa cells culture in vitro. This research consisted of two phases, the first phase was isolation and identification of andrographolide. The HeLa cells were incubated in RPMI media and Fetal Bovine Serum 10%. The concentration of andrographolide were 0 ; 0.05 ; 0.10 ; 0.25 ; 0.50 ; 1.00 ; 2.50 ; 5 ; 10 ; 25 ; 50 ; 100 ; 250 µg/ml dissolved in DMSO. Each andrographolide concentrations was added into HeLa cells culture and then incubated for 24 hours at 37oC in CO2 incubator. Apoptosis induction was determined by ethidium bromide-acridine orange exclusion and analyzed by flourecent microscope. The result of this research showed the andrografolide extracted from sambiloto (Andrographis paniculata Nees) induced apoptosis activity in HeLa cells culture in vitro with IC50 of 109.90µg/ml. The result of this experiment suggested to study the effect of andrografolide on expression of gene responsible for apoptosis mechanism in cancer cells in vitro.

      Keyword :

      Andrographis paniculata Nees, andrographolide, apoptosis,


      References :





      Archive Article

      Cover Media Content

      Volume : / No. : / Pub. :