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Media Kedokteran Hewan

ISSN 2015-8930

Vol. 22 / No. 3 / Published : 2006-09

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Original Article :

Isolation and characterization of staphylococcus aureus of milk of ettawa crossbred goat

Author :

  1. Agus Purnomo*1
  2. Hartatik*2
  3. Khusnan*3
  4. Siti Isrina Oktavia Salasia*4
  5. Soegiyono*5
  1. Akademi Peternakan Brahmaputra, Jl. Ki Ageng Pemanahan, Nitikan, Yogyakarta, Tel. 0274-384370,
  2. Akademi Peternakan Brahmaputra, Jl. Ki Ageng Pemanahan, Nitikan, Yogyakarta, Tel. 0274-384370,

  3. Akademi Peternakan Brahmaputra, Jl. Ki Ageng Pemanahan, Nitikan, Yogyakarta, Tel. 0274-384370,
  4. Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Jl. Olahraga, Karangmalang, Yogyakarta 55281
  5. Departemen Pertanian Republik Indonesia

Abstract :

Staphylococcus aureus is a main bacteria causing mastitis of Ettawa crossbred goat. The mastitis can raise an economical problem for farmers because of decreasing of milk production. S. aureus in the milk will be spreading rapidly to decompose the milk.The aims of the research were to isolate and characterize S. aureus from milk samples of Ettawa crossbred goat in Bantul, Sleman and Kaligesing. Characterization of S aureus based on the mannitol salt agar (MSA) reactions, clumping factor and coagulation assays, production of hemolysins and pigments, and antibiotic sensitivity tests. From the 52 milk samples of Ettawa crossbred goat could be isolated 12 S. aureus. All 12 isolates fermented mannitol, positive for coagulases, clumping factors, and produced -hemolysis. S. aureus isolated from Ettawa crossbred gout could produce an orange pigment for 3 isolates, yellow pigmented for 8 isolates and white pigmented for 1 isolate. Based on the sensitivity tests to the antibiotics, revealed that one isolate of S. aureus was resistent to oxytetracycline, ampicilin, erythromycin, and intermediate to gentamycin. Three S. aureus isolates were intermediate to erythromycin. Most of S. aureus were still susceptible to oxytetracycline, tetracycline gentamicin, ampicilin and erythromycin.

Keyword :

Staphylococcus aureus, Ettawa crossbred goat, milk sample,


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