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Media Kedokteran Hewan

ISSN 2015-8930

Vol. 18 / No. 2 / Published : 2002-08

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Original Article :

Monoclonal antibody production against dengue virus surabaya isolate

Author :

  1. Suwarno*1
  2. Sugeng Sugijanto*2
  3. Fedik Abdul Rantam*3
  1. Laboratorium Virologi dan Imunologi, Dengue, Tropical Disease Center, Universitas Airlangga Surabaya
  2. Laboratorium Dengue, Tropical Disease Center, Universitas Airlangga Surabaya
  3. Laboratorium Virologi dan Imunologi, Dengue, Tropical Disease Center, Universitas Airlangga Surabaya

Abstract :

The aims of this study are to isolate and characterize E protein of Dengue-3 virus Surabaya isolates, therefore can be used as to produce of monoclonal antibody. This study is divided in some periods: isolation, characterization and analysis of E protein, the production of monoclonal antibody and to determine specificity of monoclonal antibody again E protein of Dengue virus in Indonesia from different strains. The result showed that molecular weight of E protein of Dengue-3 virus Surabaya isolate was 52 kDa, and it was antigenic protein that reacted specificity to a rabbit anti Dengue-3 polyclonal antibody. Fusion of B cell and myeloma cell produced 7 clones hybridoma that secreted monoclonal antibody again E protein Dengue-3 virus, which different specificity and affinity according to the type of immunoglobuline produced.

Keyword :

Dengue virus, Surabaya isolate, specificity, polyclonal Antibody, monoclonal Antibody.,


References :



       


    Archive Article

    Cover Media Content

    Volume : 18 / No. : 2 / Pub. : 2002-08
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    9. The Nutritif Value Of Tofu Waste-rice Bran And Tofu Waste-pollard Which Was Fermented With Rhyzopus Microsporus Var. Oligosporus
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    11. The Antelminthic Effect Of Garlic (allium Sativum Linn) Against Ascaridia Galli Infection In Chicken (gallus Gallus)
    12. Monoclonal Antibody Production Against Dengue Virus Surabaya Isolate
    13. Increasing The Quality Of Gouramy Fry (osphronemus Gouramy) By Male Monosex Method