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Media Kedokteran Hewan

ISSN 2015-8930

Vol. 21 / No. 2 / Published : 2005-05

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Original Article :

Observation of bursal apoptotic cells due to avibirna virus infection

Author :

  1. Ajik Azmijah*1
  1. Laboratorium Patologi, Fakultas Kedokteran Hewan Universitas Airlangga Surabaya

Abstract :

The aim of this study is to prove the increasing amount of apoptotic cells in bursa Fabricius of chicken infected by Avibirna virus. Fifty broiler chicken of 21 day old, devided randomly into 10 groups i.e 5 groups as control and the other 5 as treated groups. Each chick of five treated groups inoculated by Tasik isolate of Avibirna virus through intraocular, intracloacal and oral routes. Subsequently both control and treated groups were sacrificed at 2nd, 4th, 6th, 8th and 10th day after infection. The bursae were taken and processed using S177101 Apopteg plus Peroxidase in situ Apoptosis Detection Kit and put into histological examination. Apoptotic cells of bursa from treated chicken which sacrificed on 4th and 6th day after infection increase sharply and differ significantly with those of control. This high amount of apoptotic cells thought to be induced by increasing number of T lymphocytes in the acute phase of viral infection

Keyword :

bursa of Fabricius, avibirna virus, apoptotic cells.,


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