UNIVERSITAS AIRLANGGA



Detail Article

Media Kedokteran Hewan

ISSN 2015-8930

Vol. 26 / No. 2 / Published : 2010-05

Order : 21, and page :124 - 127

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Original Article :

Optimization of temperature and cellulase ph from rumen bacteria isolation of beef cattle

Author :

  1. Mirni Lamid*1
  2. Ni Nyoman Tri Puspaningsih*2
  3. Widya Paramita*3
  1. Dosen Fakultas Kedokteran Hewan
  2. Dosen Fakultas Sains dan Teknologi
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

Biotechnology in animal feed using biological treatment such as cellulase is mainly aimed to increase degrade the cellulose; the second most abundant polysaccharide in nature. Information of using cellulase as biocatalyst for increasing animal feed quality as ruminants feed is limited. Cellulose is the major component of cell wall agroindustry waste after hemicellulose. This cellulase comes from Actinobacillus sp. that were isolated from rumen fluid of beef cattle. The aim of this research was to determine activity of cellulase and characterization of optimum temperature and pH. In this research identification with carboxyl methylcellulose (CMC) has been done. Methods for cellulase activity was 3,5-dinitrosalicylic acid (DNS). The results showed that Actinobacillus which have positive activity of cellulase. Characterization of Actinobacillus sp. had optimum temperature 45oC with activity 1.42 U/ml and optimum pH 6 with activity 0.57 U/ml.

Keyword :

cellulase, carboxyl methyl cellulose, temperature, beef cattle,


References :

Bhat MK and Hazlewood GP.,(2001) Enzymology and Other Characteristics of Cellulases and Xylanases, In: Enzymes in Farm Animal Nutrition M.R. Bedford and G.G. Partridge (Eds). UK : CABI Publ

Beauchemin KA, Colombatto D, Morgavi DP and Yang WZ.,(2003) Use of exogenous fibrolytic enzymes to improve feed utilization by ruminant - : J Anim Sci; 81: E37 – E47

Bondi AA.,(1987) Animal Nutrition London : Wiley-Interscience Publication

Campbell Mary K.,(1995) Biochemistry. 2nd Ed. Mount Holyoke College USA : Saunders College Publ

Lowe SE, Michael K, Theodorou and Anthony PJT.,(1987) Cellulases and Xylanases of an Anaerobic Rumen Fungus Grown on Wheat Straw, Wheat Straw Holocellulose, Cellulose, and Xylan - : Appl Environ Microbiol.

Mc. Donald P, Edwards RA and Greenhalgh JFD.,(1995) Animal Nutrition. London : Logman

Miller Gail Lorenz.,(1960) Use of Dinitrosalicylic acid reagent for determination of reducing sugar. - : Anal Chem

Orskov ER and Ryle.,



Archive Article

Cover Media Content

Volume : 26 / No. : 2 / Pub. : 2010-05
  1. Antiproliferation Effect Of The N-hexanal Extract Of Kesum (polygonum Minus) At The Cells Of The Rat Lung Cancer Effort Exploiting Of Kesum As Drug Of Lung Cancer
  2. Antiproliferation Effect Of The N-hexanal Extract Of Kesum (polygonum Minus) At The Cells Of The Rat Lung Cancer Effort Exploiting Of Kesum As Drug Of Lung Cancer
  3. Invitro Maturation Of Domestic Cat (felis Catus Ovarian Oocytes Fater Being Stored At 4o C
  4. Concentrations Of Liver And Plasma Malondealdehyde (mda) Of Sprague Dawley Rats Injected By Ketamine-xylazine Hcl Anesthetics
  5. Protein Profile Of Sporozoite Of Leucocytozoon Sp. From Culicoides Sp.
  6. Expression Of Cd4+ T Cell, Interleukin-2 And Interleukin-4 On Splenectomy Balb/c Mice Post-exposore Salmonela Typhi
  7. The Profile Of Antioxidant Superoxide Dismutase (sod) In Liver Of Isoflavon, Zn, And Vitamins E-treated Rats
  8. Species Identification Of Coagulase Positive Staphylococci (cps) By Multiplex Polymerase Chain Reaction (pcr)
  9. Species Identification Of Coagulase Positive Staphylococci (cps) By Multiplex Polymerase Chain Reaction (pcr)
  10. Mechanisms Underlying Abortion In Dexamethatione Treatment At Ewe In Midgestation
  11. Antibody Against Prolactin: As A Teraphy For Molting Duck
  12. Optimization Of Temperature And Cellulase Ph From Rumen Bacteria Isolation Of Beef Cattle
  13. Antiproliferation Effect Of The N-heksana Extract Of Kesum (polygomon Minus) At The Cells Of The Rat Lung Cancer: Effort Explotting Of Kesum As Drug Of Lung Cancer
  14. Effect Of Supplementation Of Ccows Or Direcly To Calves On Calf Performence In Bali Catlle Grazing Communal Pastures

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