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Media Kedokteran Hewan

ISSN 2015-8930

Vol. 26 / No. 2 / Published : 2010-05

TOC : 18, and page :108 - 111

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Original Article :

Species identification of coagulase positive staphylococci (cps) by multiplex polymerase chain reaction (pcr)

Author :

  1. Mustofa Helmi Effendi*1
  1. Dosen Fakultas Kedokteran Hewan

Abstract :

The aim of this work was to identify the species specific by using multiplex PCR for the identification of two species of coagulase positive staphylococci (CPS): Staphylococcus aureus, and Staphylococcus intermedius. Twenty eight staphylococcus isolates, previously characterized as CPS through the coagulase, and catalase production tests and Gram staining, were identified at genus level. At the same time, the DNA of these isolates was extracted and amplified by multiplex PCR, using primers for the nuc gene, specific for S. aureus, and S. intermedius.  It can be concluded that nuc gene can be used for differentiation between coagulase positive staphylococci.

Keyword :

Coagulase Positive Staphylococci (CPS), Staphylococcus aureus, Staphylococcus intermedius, nuc gene, multiplex PCR,


References :

  1. Akineden O, Annemuller C, Hassan A A, Lammler C, Wolter W, and Zschock M, (2001). Toxin Genes and Other Characteristics of Staphylococcus aureus Isolates from Milk of Cows with Mastitis. - : Clinical and Diagnostic Laboratory Immunology
  2. el Zubeir IEM, Kanbar T, Alber J, Lammler C, Akineden O, Weiss R, Zschock M, (2007). Phenotypic and genotypic characteristics of methicillin/oxacillin-resistant Staphylococcus intermedius isolated from clinical specimens during routine veterinary microbiological examinations. - : Veterinary Microbiology
  3. Pottumarthy S, Schapiro JM, Prentice JL, Houze YB, Swanzy SR, Fang FC, and Cookson BT, (2004). Clinical Isolates of Staphylococcus intermedius Masquerading as Methicillin Resistant Staphylococcus aureus.. - : Journal of Clinical Microbiology


   


Archive Article

Cover Media Content

Volume : 26 / No. : 2 / Pub. : 2010-05
  1. Invitro Maturation Of Domestic Cat (felis Catus Ovarian Oocytes Fater Being Stored At 4o C
  2. Concentrations Of Liver And Plasma Malondealdehyde (mda) Of Sprague Dawley Rats Injected By Ketamine-xylazine Hcl Anesthetics
  3. Protein profile of sporozoite of leucocytozoon sp. from culicoides sp.
  4. Expression Of Cd4+ T Cell, Interleukin-2 And Interleukin-4 On Splenectomy Balb/c Mice Post-exposore Salmonela Typhi
  5. The Profile Of Antioxidant Superoxide Dismutase (sod) In Liver Of Isoflavon, Zn, And Vitamins E-treated Rats
  6. Species Identification Of Coagulase Positive Staphylococci (cps) By Multiplex Polymerase Chain Reaction (pcr)
  7. Species identification of coagulase positive staphylococci (cps) by multiplex polymerase chain reaction (pcr)
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