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Journal Orthopaedi and Traumatology Surabaya

ISSN 2460-8742

Vol. 2 / No. 1 / Published : 2013-01

TOC : 10, and page :53 - 63

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Original Article :

Alloreactivity of mesenchymal stem cell transplantation in allogenic bone marrow: study about interleukin serum 10 level and its effect in inflamation reaction at muscle tissue

Author :

  1. L.G.D Anggitadewi*1
  2. Dwikora N. Utomo*2
  3. Ferdiansyah*3
  4. Fedik A. Rantam*4
  1. * Resident of Orthopedic & Traumatology Department Airlangga University / Dr.Soetomo General Hospital Surabaya
  2. Staff of Orthopedic & Traumatology Department Airlangga University / Dr.Soetomo General Hospital Surabaya.
  3. Staff of Orthopedic & Traumatology Department Airlangga University / Dr.Soetomo General Hospital Surabaya
  4. Staff of Tropical Disease Center, Stem Cells Division, Airlangga University

Abstract :

Introduction Use of allogeneic MSC for repair of large defects may be an alternative to autologous and allogeneic tissue-grafting procedures. An allogeneic approach would enable MSC to be isolated from any donor, expanded and cryopreserved, providing a readily available source of progenitors for cell replacement therapy. Current knowledge regarding the immunobiology and clinical application of MSC needs to be strengthened further to establish MSC as a safe and effective therapeutic tool in regenerative medicine. This paper discusses alloreactivity of MSCs with particular reference to serum level of  interleukin-10 (IL 10), in the engraftment process following allogenic bone marrow mesenchymal stem cells  injection to the femoral muscle, to explain  allogenic MSCs’s role as immunomodulators and their multilineage differentiation potential and possible use in tissue regeneration and repair.   Method Laboratory experimental study. Serum IL-10 levels were measured in over 16 samples  of New Zealand white rabbit, before and for up to 1 weeks following transplantation using a enzyme-linked immunoabsorbent assay (ELISA) IL-10 kit for rabbit, with a sensitivity of  ≥ 78 pg/mL. Eight white rabbit  received 2 x 106 injection of allogenic MSCs into their femoral muscle. In the remaining 8 rabbit, act as control, were injected with phosphate buffered saline. At day 8 post transplantation, injection site on femoral muscle were being obtained for histopathology examination using hematoxylin-eosine staining.   Result Histopathology examination showed no significant different found from limphocytes, monocytes, and polimorphonuclear cells between allogenic MSCs resipients and control group. Comparation of serum IL-10 peak levels were studied  in the immediate posttransplant period between day 0 and day 8 in recipients of allogenic MSCs as well as control group.   Conclusion Allogenic MSCs may modulate immunobiological process during their engraftment. This animal study showed favourable result toward  MSCs’ alloreactivity.    

Keyword :

allogeneic mesenchymal stem cells, interleukin 10, immunobiology, tissue repair,


References :

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  4. 9. Nauta AJ, Westerhuis G, Kruisselbrink AB, Lurvink EG, Willemze R, Fibbe WE, (2006). Donor-derived mesenchymal stem cells are immunogenic in an allogeneic host and stimulate donor graft rejection in a non myeloablative setting. - : Blood
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Archive Article

Cover Media Content

Volume : 2 / No. : 1 / Pub. : 2013-01
  1. Alloreactivity of mesenchymal stem cell transplantation in allogenic bone marrow: study about interleukin serum 10 level and its effect in inflamation reaction at muscle tissue
  2. Association between characteristic, antropometric, behavior, psychosocial status and radiographic of the responden with spondylogenic low back pain cases