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Journal Orthopaedi and Traumatology Surabaya

ISSN 2460-8742

Vol. 2 / No. 2 / Published : 2013-08

TOC : 16, and page :118 - 122

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Original Article :

Comparison of fibroblats cell counts in rabbit’s gastrocnemius muscle healing which treated with bone marrow derived mesenchymal stem cells and without mesenchymal stem cells

Author :

  1. IGL Krishna Mahayana*1
  2. Heri Suroto*2
  3. Sjahjenny Mustokoweni*3
  1. Resident of Orthopaedic &Traumatologi Dept, Dr. Soetomo General Hospital, Airlangga Univ. School of Medicine, Surabaya
  2. Staff of Orthopaedic &Traumatologi Dept, Dr. Soetomo General Hospital, Airlangga Univ. School of Medicine, Surabaya
  3. Staff and Senior consultant in Pathology Anatomy Department, School of Medicine Airlangga University, Surabaya

Abstract :

ABSTRACT Background : Injury of muscle tissue has become a difficult situation in traumatic case and muscle disease. It happen due to slow rate of healing. Recent development in basic science, especially stem cells and regenerative medicine, bringing new hope in such problem. his study tested on hypothesis which bone marrow derived mesenchymal stem cells able to boost muscle healing in injured rabbit muscle. Materials and Methods : The goal in this study was to compare the fibroblast cell counts in the injured part of a rabbit’s gastrocnemius muscle which was injected with stem cells with muscle that did not receive stem cells injection. Design of this study is post-test only control group. Type of the animals used here are New Zealand White rabbits .Shape of the injury was sharp and completely transected through midsubstance of gastrocnemius muscle. The injury  repaired with running  suture of the epimysium using pro lene 4.0 and prolene 5.0 for suture of the fascia . The left gastrocnemius muscle  treated with  bone marrow derived stem cells and  the right  limb treated without any stem cells then two weeks later the specimens were harvested . Results : Fibroblast cells counts of the group  that was injected with mesenchymal stem cells increased significantly compare to the other group. Conclusions : Fibroblast cells number markedly increase inside the injured site of gastrocnemius muscle that was injected with bone marrow derived mesenchymal stem cells.

Keyword :

Muscle healing, fibroblast cells, mesenchymal stem cells, -, -,

References :

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  5. Tajbakhsh S, (2009). Skeletal muscle stem cells in developmental versus regenerative myogenesis. - : Journal Internal Medicine


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