Catalog :

Detail Article

Journal Orthopaedi and Traumatology Surabaya

ISSN 2460-8742

Vol. 1 / No. 1 / Published : 2012-01

TOC : 9, and page :48 - 52

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Original Article :

The effect of bone marrow derived mesenchymal stem cells of the fibrotic tissue space of the healing gastocnemius muscle of a rabbit

Author :

  1. Jeffry Andrianus*1
  2. Tri Wahyu Martanto*2
  1. Resident of Orthopaedic and Traumatology Department
  2. Senior Consultant of Orthopaedic and Traumatology Department

Abstract :

Background :Tissue engineering techniques have shown promise in the treatment of muscleinjury. The present study tested the hypothesis that bone marrow derived mesenchymal stemcells can accelerate and modulate muscle healing proccess after primary repair of muscleinjury in rabbit models.Materials and Methods : In this studywe use a post-test only control group design study.Fiveteen New Zealand White rabbits were used as experimental animals. The injury modelwas sharp complete transected through midsubstance of gastrocnemius muscle. Thetransected muscle immediately repaired with running suture of the epimysium using prolene4.0 and a primary suture of the fascia using prolene 5.0. Both lower limb were used, the leftgastrocnemius muscle receive bone marrow derived stem cells and the counter part limbwith no mesenchymal stem cells. Specimens were harvested at fourth weeks.The purpose ofthis work was to evaluate the fibrotic tissue space in the repair site of a given stem cells of arabbit’s gastrocnemius muscle.Results : Analyzed with Kolmogorov-Smirnov and Shapiro Wilk test, all groups have anormal distribution (p>0,05). Analyzed with Independent t-test, there were significantdifferences between the two groups in the histological evaluation on fibrotic tissue space atrepair site of transected gastrocnemius muscle compared to control muscle (p=0,008).Conclusions :Intramuscular cell therapy with bone marrow derived mesenchymal stemcellsfollowing primary muscle repair,minimalized the fibrotic tissue space within repair siteof the rabbit’s gastrocnemius muscle.

Keyword :

Mesenchymal stem cells, gastrocnemius muscle healing, fibrotic tissue space,

References :

  1. J. Menetrey, C. Kasemkijwattana, C. S. Day, P. Bosch, M. Vogt, F. H. Fu, M. S. Moreland, J. Huard, (2000). Growth factors improve muscle healing in vivo. - : JBJS
  2. Lidiane Isabel Filippin , Andrea Janz Moreira , Norma Possa Marroni , Ricardo Machado Xavier, (2009). Nitric oxide and repair of skeletal muscle injury. - : Elseviere
  3. William E. Garrett, Jr, Thomas M. Best, (1998). Anatomy, Physiology, and Mechanics of Skeletal Muscle”. Chapter 26 , Orthopaedi Basic Science, Biology and Biomechanics of the Musculoskeletal System 2nd Edition. - : AAOS
  4. Stefano Ciciliot, and Stefano Schiaffino, (2010). Regeneration of Mammalian Skeletal Muscle: Basic Mechanisms and Clinical Implications. - : Current Pharmaceutical Design
  5. Mauro Krampera, Giovanni Pizzolo, Giuseppe Aprili, Massimo Franchini, (2006). Mesenchymal stem cells for bone, cartilage, tendon and skeletal muscle repair. - : j.bone


Archive Article

Cover Media Content

Volume : 1 / No. : 1 / Pub. : 2012-11
  1. Comparison of tgf β expression in tendon achilles rupture reparation healing process with and without mesencymal stem cell at rabbit's spinal marrow
  2. Scleraxis as determinant of tendon cell density in achilles tendon healing treated with mesenchymal stem cell in rabbit
  3. The effect of n-acetylcysteine (nac) on tissue malondialdehyde (mda) level and tissue edema in ischemia reperfusion injury of rat (rattus norvegicus) skeletal muscle
  4. The comparison of biomechanical properties between autograft flexor tendon with freeze dried flexor tendon composite and auto mesenchymal stem cell for the reconstruction of flexor tendon defect
  5. Comparison of outcomes between nonoperative treatment and k-wire fixation of central metatarsal fractures
  6. The comparison in the amount of neovascularization between autograft flexor tendon with freeze dried flexor tendon allograft composite and auto mesenchymal stem cells in the reconstruction of flexor tendon defect in new zaeland white rabbit
  7. The effect of bone marrow derived mesenchymal stem cells of the fibrotic tissue space of the healing gastocnemius muscle of a rabbit
  8. Evaluation of the association of vertebrae fracture level with deficit degree in rsud dr soetomo inpatient januari 2010 - desember 2010