Detail Article


ISSN 2302-6464

Vol. 3 / No. 2 / Published : 2014-10

Order : 11, and page :274 - 280

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Original Article :

Addition of cattle seminal plasma to sperm quality goat on freezing process with time equilibration different

Author :

  1. Suherni Susilowati*1
  2. Trilas Sardjito*2
  3. Indah Norma Triana*3
  1. Dosen Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

Equilibration is the time required for adjustment spermatozoa with diluent and glycerol. Long equilibration can affect the quality of spermatozoa in the clotting process. In addition to the current freezing of goat semen has not been satisfactory because as in goat seminal plasma contained enzyme phospholipase A and lipase enzymes trigliserol can reduce the motility of spermatozoa in milk diluents. The purpose of this study is to investigate the addition of seminal plasma on spermatozoa cow goat centrifugation results in a dilution of milk with different equilibration time in the freezing process. Cattle seminal plasma obtained from cows cement shelter, then the plasma was separated and then used for processing. Cattle seminal plasma was then added to the centrifugation results goat spermatozoa diluted with skim milk. This study consisted of three groups: Control group P0 : Addition of seminal plasma in the cow goat spermatozoa centrifugation results in dilution of skim milk with equilibration time of 1 hour, Group P1: Addition of seminal plasma in the cow goat spermatozoa centrifugation results in dilution of skim milk with equilibration time 2 hours. Group P2: the addition of seminal plasma in the cow goat spermatozoa centrifugation results in dilution of skim milk with equilibration time of 3 hours. Then examined motility, viability and plasma membrane integrity of spermatozoa. The results showed that the addition of seminal plasma of cattle maintain motility, viability and plasma membrane intact spermatozoa in diluents goat milk with different equilibration times in the clotting process.

Keyword :

Seminal Plasma cow, goat spermatozoa, equilibration, Motility, Viability,

References :

Blanco,T.M, Rperez-Pe and JA Cebrian Perez. ,(2008) Seminal Plasma Protein and Sperm Resistence to Stress. USA : Reprod Dom Anim 43(4).18-31.

Colas,C,R Perez-Pe, A. Casao,M. Ollero, L. Calleja, M. Gallego, T. M.Blanco and J.A. cebrian-Perez. ,(2012) Remodelling of Lipid Rafts During In Vitro Capacitation and Acrosome Reaction of Ram Spermatozoa. USA : Biochemistry and Analytical Biochemistry. 55-001

Gazali,M dan Tambing ,S.N. ,(2002) Cryopreservation of Sperm. Indonesia : Hayati. Vol 9.No1. Hal 27-32.

Graham, J.K and Philip,H.P. ,(2004) Effect of Adding Cholesterol to Bull Sperm Membranes on Sperm Capacitation, the Acrosom Reaction and Fertility. USA : Biol Reprod 71:522-527.

Archive Article

Cover Media Content

Volume : 3 / No. : 2 / Pub. : 2014-10
  1. Measurement Of Nuclear Dna Damage Of Simental Bull Spermatozoa Post Thawing With Sentrifuged Egg Yolk Skim And Soybean Lecitin Diluter
  2. Viability Of Spermatozoa Merino Sheep With Addition Of Various Nacl Concentration
  3. Numbers Resistance Spermatozoa Of Merino Rams And Etawa Goat In Nacl 0.9%, Nacl 1% And Nacl 1.1%
  4. Response To The Onset Of Estrous After Revocation Mpa (medroxy Progesterone Acetate) Intravaginal With Followed By Giving Gonadotropin Hormone On Fat Tailed Sheep
  5. Effect Of Pregnant Mare Serum Gonadotropin (pmsg) In Combination With Follicle Stimulating Hormone (fsh) For Superovulatory Treatment On The Onset And Duration Of Estrus In Fat Tailed Ewes (ovis Aries)
  6. The Effect Of Various Thawing Time On Frozen Semen Of Fat Tailed Sheep For Viability, Motility And Intact Plasma Membrane Of Spermatozoa
  7. Efforts To The Revenue Livestock Laying Duck And Fight Through The Surface Water Pollution Feed Management, Waste And Diversified Business
  8. Reproduction Efficiency Of Artificial Insemination Beef Cattle In East And West District, Kabupaten East Lombok On 2012
  9. Reproductive Efficiency Of Limousin And Simmental Cross Resulted From Artificial Insemination (ai) During 2012 In Ngoro, Jombang
  10. The Influence Of Mangosteen Peels Infusion (garcinia Mangostana L.) To Acrosome Reaction On Rats Spermatozoa
  11. Addition Of Cattle Seminal Plasma To Sperm Quality Goat On Freezing Process With Time Equilibration Different
  12. Identification Fertility Associated Antigen (faa) In Seminal Vesicle Of Brahman Bull Using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (sds-page) And Western Blot
  13. Isolation And Identification Of Osteopontin Sperm Membrane An Effort To Improve The Quality Of Holstein Fressian Dairy Bull Frozen Semen
  14. Suplementation Of Insuline-like Growth Factor-i Pregnant Thoroughbred Mare Serumon Increase Of Bovine Embrio Cleavage Rates