OVOZOA
ISSN 2302-6464
Vol. 3 / No. 2 / Published : 2014-10
Order : 11, and page :274 - 280
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Original Article :
Addition of cattle seminal plasma to sperm quality goat on freezing process with time equilibration different
Author :
- Suherni Susilowati*1
- Trilas Sardjito*2
- Indah Norma Triana*3
- Dosen Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
Abstract :
Equilibration is the time required for adjustment spermatozoa with diluent and glycerol. Long equilibration can affect the quality of spermatozoa in the clotting process. In addition to the current freezing of goat semen has not been satisfactory because as in goat seminal plasma contained enzyme phospholipase A and lipase enzymes trigliserol can reduce the motility of spermatozoa in milk diluents. The purpose of this study is to investigate the addition of seminal plasma on spermatozoa cow goat centrifugation results in a dilution of milk with different equilibration time in the freezing process. Cattle seminal plasma obtained from cows cement shelter, then the plasma was separated and then used for processing. Cattle seminal plasma was then added to the centrifugation results goat spermatozoa diluted with skim milk. This study consisted of three groups: Control group P0 : Addition of seminal plasma in the cow goat spermatozoa centrifugation results in dilution of skim milk with equilibration time of 1 hour, Group P1: Addition of seminal plasma in the cow goat spermatozoa centrifugation results in dilution of skim milk with equilibration time 2 hours. Group P2: the addition of seminal plasma in the cow goat spermatozoa centrifugation results in dilution of skim milk with equilibration time of 3 hours. Then examined motility, viability and plasma membrane integrity of spermatozoa. The results showed that the addition of seminal plasma of cattle maintain motility, viability and plasma membrane intact spermatozoa in diluents goat milk with different equilibration times in the clotting process.
Keyword :
Seminal Plasma cow, goat spermatozoa, equilibration, Motility, Viability,
References :
Blanco,T.M, Rperez-Pe and JA Cebrian Perez. ,(2008) Seminal Plasma Protein and Sperm Resistence to Stress. USA : Reprod Dom Anim 43(4).18-31.
Colas,C,R Perez-Pe, A. Casao,M. Ollero, L. Calleja, M. Gallego, T. M.Blanco and J.A. cebrian-Perez. ,(2012) Remodelling of Lipid Rafts During In Vitro Capacitation and Acrosome Reaction of Ram Spermatozoa. USA : Biochemistry and Analytical Biochemistry. 55-001
Gazali,M dan Tambing ,S.N. ,(2002) Cryopreservation of Sperm. Indonesia : Hayati. Vol 9.No1. Hal 27-32.
Graham, J.K and Philip,H.P. ,(2004) Effect of Adding Cholesterol to Bull Sperm Membranes on Sperm Capacitation, the Acrosom Reaction and Fertility. USA : Biol Reprod 71:522-527.
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