UNIVERSITAS AIRLANGGA



Detail Article

OVOZOA

ISSN 2302-6464

Vol. 3 / No. 1 / Published : 2014-04

Order : 4, and page :161 - 167

Related with : Scholar   Yahoo!   Bing

Original Article :

Determination encoding genes heat stable toxin (st) and protein of pathogens escherichia coli pili k99 was isolated from dairy cow feces using polymerase chain reaction (pcr)

Author :

  1. Nadya Ayu Kusuma*1
  2. Didik Handijatno*2
  3. Setiawan Koesdarto*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

Infection with enterotoxigenic Escherichia coli (ETEC) is one of the main causes of neonatal calf diarrhea in developing countries. The major virulence factors of enterotoxigenic strains of Escherichia coli in neonatal is also the heat-stable enterotoxin (ST) and K99 pili proteins. In a case control study Esherichia coli isolated from faecal samples from dairy calves were phenotyped by biochemical fingerprinting and analyzed for virulence genes by PCR. PCR assays, which are more rapid, sensitive, and specific, have also been widely used for ETEC diagnosis. This research used non probability sampling. The samples taken from 10 dairy farmers placed in Malang who have cases of death in calves and poor sanitation criteria. Samples were firstly plated on Eosyn Methylene Blue Agar (EMBA), and afterwards the colonies that suspect Escherichia coli (colored metallic green) confirmed by Indol test, the other biochemistry test, and Blood Agar (BA) enrichment media. Positively test, later was  tested by PCR by using primer ST and primer K99. Results of this research showed that all 10 samples of suspect Escherichia coli on media EMBA and 7 of the 10 positive samples in Indol test and other Biochemistry test. Results of electrophoresis of PCR products from 7 positive samples of Escherichia coli, 3 samples showed positive for the presence of genes encoding ST amplicon 147 bp in length. While the results of PCR using primers K99 nothing positive. Based on the test result  ETEC in the samples may not indicate the presence gene encoding K99 pili proteins.

Keyword :

Escherichia coli, enterotoxin Escherichia coli (ETEC), , ST, K99, PCR.,


References :

Brenjchi, M., A. Jamshidi, N. Farzaneh, and M. R. Bassami.,(2011) Identification of Shiga Toxin Producing Escherichia coli from Dairy Farms in Mashhad using Multiplex PCR Assay. Iran : Iranian J of Vet. Ressearch. 12(2): 35.

Franck, S.M., Brad T. Bosworth, Harley W. Moon. ,(1998) Multiplex PCR for Enteroxigenic, Attaching and Effacing, and Shiga Toxin-Producing Escheri-chia coli Strains from Calves. USA : J. Clin. Microbiology. Vol. 36, No. 6.

Wang J., S.W. Jiang, X.H. Chen, Z.L. Liu, J. Peng. ,(2006) Prevalence of Frimbial Antigen (K88 variants, K99 and 987P) of Enteroxigenic Escherichia coli from Neonatal and Post-weaning Piglets with Diarrhea in Central China. USA : J. Anim. Sci. Vol. 19, No. 9: 1342-1346.





Archive Article

Cover Media Content

Volume : 3 / No. : 1 / Pub. : 2014-04
  1. Morning And Evening Serum Testosterone Level Of Limousine Crossbreed Bulls
  2. Test Potential Polyclonal Antibodies Local (abpo Local Pmsg) And Patents (abpo Patent Pmsg) Pmsg (pregnant Mare Serum Gonadotropin) From Male Rabbit (oryctolagus Cuniculus) To Pregnancy Mice (mus Musculus)
  3. Dna Fragmentation As Apoptotic Indicator And It Relation With Sperm Viability And Motility Of Domba Ekor Gemuk Frozen Semen In Different Thawing Duration
  4. Determination Encoding Genes Heat Stable Toxin (st) And Protein Of Pathogens Escherichia Coli Pili K99 Was Isolated From Dairy Cow Feces Using Polymerase Chain Reaction (pcr)
  5. Biopotensi Pmsg (pregnant Mare Serum Gonadotrophin) Local Horse To Speed Time Arising Estrus, Number Of Follicles, Corpus Luteum, Progesterone Levels And Pregnancy Rate In Madura Cattle
  6. Potential Of Vitamin E (α-tocopherol) Of Malondialdehyde (mda) Levels Testis Mice (mus Muscular) Induced 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (tcdd)
  7. Effect Of “sarang Semut” (myrmecodia Pendans) On The Number Of Spermatogenic Cells In Seminiferous Tubules Of Mice (mus Musculus) With Excessive Physical Treatment
  8. The Effect Of Estrous Syncronization By Pgf2α And Superovulation By Pmsg And Hcg And Artificial Insemination On Heat Response, Pregnancy Rates, Litter Size And Sex Ratio Of The Crossbread Etawa Goat
  9. Examination Of Fresh Semen Quality Of Merino Sheep For Frozen Semen Production In Teaching Farm Of Veterinary Medicine Faculty Of Airlangga University
  10. Identification Of Sperm Morphometry In Merino Sheep And Fat-tailed Sheep
  11. The Correlation Between Body Weight With Body Lenght, Wither’s Height, Heart Girth, Scrotal Circumference, Volume, And Semen Quality Of Peranakan Etawa Goat
  12. Profile Of Progesterone And Insulin-like Growth Factor-i (igf-i) In Pregnant Crossbred Mare Serum
  13. Effect Of Equilibration Time Extender To Post Thawing Motility And Viability Of Merino Sheep’s Sperms In Egg Yolk Skim Diluents
  14. The Effect Of The Combination Progesterone In Sponge Intravaginal With Equine Chorionic Gonadotropin (ecg) To The Occurrence Of Estrous On Fat Tailed Sheep

Information OVOZOA