OVOZOA
ISSN 2302-6464
Vol. 2 / No. 1 / Published : 2013-04
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Original Article :
Effect of addition protein fertility associated antigen (faa) in semen on capacitation and acrosome reaction spermatozoa in bovine frozen semen
Author :
- Tri Wahyu Suprayogi*1
- Trisnawati Wedya Pratiwi*2
- Indah Norma Triana*3
- Dewa Ketut Meles*4
- Departemen Reproduksi Fakultas Kedokteran Hewan Unair
- Mahasiswa Fakultas Kedokteran Hewan Unair
- Departemen Kedokteran Dasar Fakultas Kedokteran Hewan Unair
- Departemen Kedokteran Dasar Veteriner Fakultas Kedokteran Hewan
Abstract :
ABSTRACT The aims of this study was to determined the effect of addition protein Fetility Associated Antigen (FAA) in dilution on capacitation and acrosome reaction spermatozoa post thawing in frozen semen. This study were used frozen semen sampel of bovine in a straw form that divided into 4 treatment, control without the addition of the FAA (P0), the addition of the FAA with a dose 5 µg (P1), the addition of the FAA with a dose 10 µg (P2) and the addition of FAA with a dose 15 µg (P3). After be thawing then performed capacitation and acrosome reaction spermatozoa. Data were analyzed with Analysis of Variance (ANOVA), followed by Duncan's multiple range test. The study results of capacitation and acrosome reaction percentages were showed significant differences between treatments (p> 0.05). The conclusions of this study were in addition of the Fertility Associated Antigen (FAA) with a dose 15 µg were able decreased capacitation and acrosome reaction spematozoa.
Keyword :
frozen semen, , FAA, plasma membrane, capacitation, acrosome reaction,
References :
McCauley et al. ,(1999) Purification an Caracteritation of Fertily-Associated Antigen (FAA) in Bovine Seminal Fluid, Molecular Reproduction and Development. 54:145-153. USA : Molecular Reproduction and Development.
Park. J.E. and J.K. Graham. ,(1992) Effect of Cryopreservation Proce-dures on Sperm Membranes. J.Theriogenology 38:209-222. USA : Theriogenology
Lenz, R. W., H. Zhang, J.N. Oyarzo, M.E. Bellin, and R.L. Ax. ,(2000) Bovine Fertility-Associated Anti-gen (FAA) and a Recom-binant Segment of FAA Improved Sperm Function. SSR, Abstract 80. USA : SSR
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