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OVOZOA

ISSN 2302-6464

Vol. 2 / No. 2 / Published : 2013-10

TOC : 4, and page :24 - 31

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Original Article :

Effect of glycerol in skim egg yolk diluent on the freezing of goldfish spermatozoa (cyprinus carpio)

Author :

  1. Dewi Alima Nostalia Suseno*1
  2. Abdul Samik *2
  3. Lita Rakhma Yustinasari *3
  1. Mahasiswa Fakultas Kedokteran Hewan Unair
  2. Departemen Reproduksi Veteriner Fakultas Kedokteran Hewan Unair
  3. Departemen Anatomi Veteriner Fakultas Kedokteran Hewan Unair

Abstract :

Glycerol plays an important role in the processing of frozen semen. Glycerol is one of the intracellular cryoprotectants, which is able to replace the water in the cell and shift the electrolytes out of the cell. Egg yolk as extracellular protection provided by lipoproteins forward to protect spermatozoa from cold shock during the freezing process by stabilizing the sperm membrane. The aims of his study to define the best concentration of glycerol in the skim egg yolk diluents for the motility and viability of carp (Cyprinus carpio) spermatozoa pre-freezing and post-thawing. Glycerol concentrations were used 11%, 13% and 15% in diluter B skim egg yolk. This research using statistical test with completely randomized full designed by six replicants and had been analyzed by ANOVA (analysis of variance) and continued with Honestly Significant Difference test (HSD) at a significance level of 5%. The results showed that the use of different concentrations of glycerol had no effect on carp sperm motility and viability. The highest average percentage obtained with 15% glycerol concentration was 53.83 ± 17.497 for motility, and 67.50 ± 17.026 for viability. The lowest motility and viability were obtained with 11% glycerol was of 49.16 ± 9.174 for motility and 63.66 ± 7.339 for viability.

Keyword :

glycerol concentration , skim yolk, sperm carp (Cyprinus carpio) quality,


References :

  1. Herdis, M. R. Toelihere, I. Supriatna , B. Purwantara dan R. T. S. Adikara., (2005). Optimalisasi Kualitas Semen Cair Domba Garut (Ovis aries) melalui Penambahan Maltosa ke dalam Pengencer Semen Tris Kuning Telur. . Surabaya : Media Kedokteran Hewan Vol. 21 No.2.
  2. Zairin, M., S. Handayani dan I. Supriatna. , (2005). Kualitas Sperma Ikan Batak (Tor Soro) Hasil Kriopreservasi Semen Menggunakan Dimetilsulfoksida (Dmso) dan Gliserol 5, 10 dan 15%. . Jakarta : Jurnal Akuakultur Indonesia 4 (2): 125-151.


   


Archive Article

Cover Media Content

Volume : 2 / No. : 2 / Pub. : 2013-10
  1. Extract of annona muricata as egg yolk citrate diluent substitute for fat-tailed sheep sperm quality
  2. Effect of different injection time of pregnant mare serum gonadotropin (pmsg) on the onset of estrous of fat tailed ewes synchronized using intravaginal progesterone
  3. The effect of a diluent contained lecithin on the quality post thawing carp’s (cyprinus carpio) spermatozoa
  4. Effect of glycerol in skim egg yolk diluent on the freezing of goldfish spermatozoa (cyprinus carpio)
  5. The effect of clove tea ( syzygium aromaticum ) on histopathological view of mouse (mus musculus) testis with cigarette smoke exposured
  6. Extract banana an as alternative fructose toward a quality of fat-tailed shepp’s sperm
  7. Fertilization rate of merino sheep frozen semen in diluent egg yolk tris and egg yolk skim to in vitro fertilization
  8. Reproductive efficiency of beef cattle resulted from artificial insemination in wringin subdistrict, bondowoso district in 2012
  9. Artificial insemination program for beef cattle in madura island “targets, realisation and problems”
  10. Effect of infusum salam leaves (eugenia polyantha) testis spermatogenic cells amount of white rats (rattus norvegicus) induced alloxan
  11. The effect of bitter melon fruit (momordica charantia l.) extract on pregnancy rate and litter size of mice (mus musculus)
  12. The effect of giving protein fertility associated antigen (faa) to capacitation and acrosome reaction on frozen semen simental bull after centrifugation
  13. Effect of seminal plasm of cow on goat’s spermatozoa motility and viabilityin in milk diluter
  14. The effect of caffeine in washing and capacitation media of the goat spermatozoa on pregnancy rates of local goat