Detail Article


ISSN 2302-6464

Vol. 3 / No. 2 / Published : 2014-10

Order : 12, and page :281 - 285

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Original Article :

Identification fertility associated antigen (faa) in seminal vesicle of brahman bull using sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) and western blot

Author :

  1. Ruswita Permana Sari*1
  2. Anwar Ma’ruf*2
  3. Rr. Sri Pantja Madyawati*3
  4. Tri Wahyu Suprayogi*4
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan
  4. Dosen Fakultas Kedokteran Hewan

Abstract :

Fertility Associated Antigen was a protein which had a function as a facilitator in the capacitation of spermatozoa during ejaculation. The presence of this in membrane plasma spermatozoa and plasma seminalis could increase the fertility of the bull. The aim of this study was to identify Fertility Associated Antigen (FAA) in the accessory sex glands of bull (the source of seminal plasma). This research used accessory sex glands from nine bulls. It was obtained from slaughterhouse in Ketanon, Tulungagung, extracted using muller mortar and mixed with PBS. That samples were identificated using the SDS-PAGE and Western Blot technique for more specific result. The result from SDS PAGE showed 9 protein bands, namely: 181 kDa, 73,53 kDa, 66,33 kDa, 54,79 kDa, 44,40 kDa, 35,76 kDa, 30,22 kDa, 24,79 kDa and 19,12 kDa. From that result here were several protein bands of proteins with molecular weight of 30,22 kDa which identified as Fertility Associated Antigen (FAA) after being tested by monoclonal antibody anti-FAA. The conclusion from this research was that Fertility Associated Antigen (FAA) could be identified on accessory sex glands of bull.

Keyword :

Accessory Sex Gland, bull, FAA, SDS-PAGE, Western Blot,

References :

Bellin, M.E., J.N. Oyarzo, H.E. Hawkins, H. Zhang, R.G. Smith, D.W. Forrest, L.R. Sprott, and R.L. A,(1998) Fertility Associated Antigen on Bull Sperm Indicates Fertility Potential. USA : J Anim Sci, 76:2032-2039.

Harmini, R.W. Asmarantaka, dan J. Atmakusuma. ,(2011) Model Dinamis Sistem Ketersediaan Daging Sapi Nasional. Indonesia : Jurnal Ekon Pemb. 12 (1) :128-146.

Mccauley, T. D., H. Zhang, M.E. Bellin, dan R.L. AX. ,(1999) Purification and Characterization of Fertility Associated Antigen (FAA) in Bovine Seminal Fluid. USA : Molec. Reprod and Develop. 54:145–153

Noyes, J. H., K. Bruce, Johnson, D. Larry, Bryant, L. Scott, Findholt, J. W. Thomas. ,(2011) Effects of Bull Age on Conception Dates and Pregnancy Rates of Cow Elk. USA : J Wildl Manage, 60 (3) : 508-517

Sprott, L. R., M.D. Harris, D.W. Forrest, J. Young, H.M. Zhang. J.M. Oyarzo, M.E. Bellin, and R.L. A,(2000) Artificial Insemination Outcomes in Beef Females Using Bovine Sperm with a Detec

Archive Article

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Volume : 3 / No. : 2 / Pub. : 2014-10
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  12. Identification Fertility Associated Antigen (faa) In Seminal Vesicle Of Brahman Bull Using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (sds-page) And Western Blot
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