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OVOZOA

ISSN 2302-6464

Vol. 3 / No. 2 / Published : 2014-10

TOC : 13, and page :286 - 293

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Original Article :

Isolation and identification of osteopontin sperm membrane an effort to improve the quality of holstein fressian dairy bull frozen semen

Author :

  1. Tatik Hernawati*1
  2. Abdul Samik*2
  3. Erma Safitri*3
  1. Dosen Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

Long term purpose of this research is a new product of freezing media for improving bull’s frozen semen fertility. If freezing media with supplemetation of osteopontin protein from this research proven can improve fertility, all bull in Balai Inseminasi Buatan could use this media as freezing media in frozen semen processing. Fertility improvement are guidance for producing higher pregnacy rate in cattle. Some of researchs show that male with semen contain osteopontin, a sperm membrane protein with molecule weight 55 kDa has higher fertility more than 20 to 25 % if compared by male semen without osteopontin. For that reason it needed to improve fertility of semen by adding osteopontin isolated from sperm membrane. This research aim is to describe the mechanism of improved bull’s fertility using spermatozoa saved in freezing media with supplementation of osteopontin from  sperm membrane to increase the numbers of offspring, at the end to increase dairy cattle population in Indonesia rapidly. To fulfill this expectation needed sequence of research gradually. First research : biochemistry characterization and identification toward osteopontin isolated from bull’s sperm membrane include osteopontin expression in bull’s semen using osteopontin monochlonal antibody, determination of molecule weight of osteopoetin by SDS-PAGE confirmed with Western blot method using osteopoetin monochlonal antibody.

Keyword :

osteopontin, sperm membrane, protein characterization,


References :

  1. Denhardt, D.T., (2004). The third interna-tional conference on osteopontin and related proteins, . San Antonio, Texas. : Calcif. Tiss. Int. 74: 213–219.
  2. Erikson D.W., A.L. Way, R.P. Bertolla, D.A. Chapman and G.J. Killian. , (2007). Influence of osteopontin, casein and oviductal fluid on bovine sperm capacitation. . USA : Anim.Reprod 4:103-112
  3. Esteves,S.C., D.M. Spaine,A.P. Cedenho, and M. Srough., , (2005). Effects of The Technique of Cryopreservation and Dilution/Centrifugation After Thawing on The Motility and Vitality of Sper-matozoa of Oligoasthenozoospermic Men. . Brazil : International Braz.J. Urol. 29 : 133-40.
  4. Gabler, C. D.A. Chapman and G.J. Killian. , (2003). Expression and presence of osteopontin and integrins in the bovine oviduct during the estrous cycle. . USA : Reproduction 126: 721–729.
  5. Ghosh, P.S. Chattopadhyay and S.Batabyal. , (2008). Immunobiochemical characteri-zation of 55 kDa fertility associated protein of Garole Sheep (Ovis aries) seminal plasma. . India : The Indian Journal of Veterinary Research 17.
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  7. Kim S and T Shin. , (2007). Immunohisto-chemical study of osteopontin in boar testis. . USA : J. Vet. Sci. 8: 107–110.
  8. Moura, A.A., H. Koc, D.A. Chapman and G.J. Killian, G.J., , (2007). A comprehensen proteomic analysis of the accessory sex gland fluid of mature Holstein bulls. . USA : Anim. Reprod. Sci. 98: 169–188.
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Archive Article

Cover Media Content

Volume : 3 / No. : 2 / Pub. : 2014-10
  1. Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter
  2. Viability of spermatozoa merino sheep with addition of various nacl concentration
  3. Numbers resistance spermatozoa of merino rams and etawa goat in nacl 0.9%, nacl 1% and nacl 1.1%
  4. Response to the onset of estrous after revocation mpa (medroxy progesterone acetate) intravaginal with followed by giving gonadotropin hormone on fat tailed sheep
  5. Effect of pregnant mare serum gonadotropin (pmsg) in combination with follicle stimulating hormone (fsh) for superovulatory treatment on the onset and duration of estrus in fat tailed ewes (ovis aries)
  6. The effect of various thawing time on frozen semen of fat tailed sheep for viability, motility and intact plasma membrane of spermatozoa
  7. Efforts to the revenue livestock laying duck and fight through the surface water pollution feed management, waste and diversified business
  8. Reproduction efficiency of artificial insemination beef cattle in east and west district, kabupaten east lombok on 2012
  9. Reproductive efficiency of limousin and simmental cross resulted from artificial insemination (ai) during 2012 in ngoro, jombang
  10. The influence of mangosteen peels infusion (garcinia mangostana l.) to acrosome reaction on rats spermatozoa
  11. Addition of cattle seminal plasma to sperm quality goat on freezing process with time equilibration different
  12. Identification fertility associated antigen (faa) in seminal vesicle of brahman bull using sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) and western blot
  13. Isolation and identification of osteopontin sperm membrane an effort to improve the quality of holstein fressian dairy bull frozen semen
  14. Suplementation of insuline-like growth factor-i pregnant thoroughbred mare serumon increase of bovine embrio cleavage rates