UNIVERSITAS AIRLANGGA



Detail Article

OVOZOA

ISSN 2302-6464

Vol. 3 / No. 2 / Published : 2014-10

Order : 1, and page :225 - 230

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Original Article :

Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter

Author :

  1. Novia Candrawati*1
  2. Suherni Susilowati*2
  3. Bambang Poernomo S.R.*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The research was aimed to compare egg yolk skim and soybean lecithin diluter after sentrifugation. Comparison both diluter measured by nuclear DNA damage of Simental bull spermatozoa. All parameter were measured before and after sentrifugation. Apoptotic sperm nuclear damage was measured by TUNNEL assay metode using Apo-BrdU-IHCTM In Situ DNA Fragmentation Assay Kit.  The result of evaluation of nuclear DNA damage showed significant difference (p<0,05), it was perhaps caused by the composition of soybean lecithin diluter was more complete with the present of antioxidant. The result of spermatozoan quality measurement showed there were no significant difference except for the motility that showed significant difference before sentrifugation (p<0,05).

Keyword :

Diluents, centrifugation, sperm nuclear DNA,


References :

Darmawan, H. ,(2007) Production of ROS and its effect on mitochondrial and nuclear DNA, human spermatozoa, and sperm function. Indonesia : Media Jurnal Indonesia.16 (2):127-133.

Hadi, R.S.,(2011) Apoptosis Pada Sperma Sebagai Petanda Adanya Gangguan Kesuburan Pria. Indonesia : Majalah Kesehatan Pharma Medika. 3(2): 282-285.

Ilyas, S. ,(2007) Analysis of protein fas expression and caspase 3 activated at the suppression phase to sperm quantity by androgen/progestin combination. Sumatera : Jurnal Biologi Sumatera. 2 (2): 45 – 47.

Rizal, M., Maman, S., Herdis, dan Achmad, S.A.,(2006) Peranan Plasma Semen dalam Mempertahankan Kualitas Spermatozoa Asal Epididimis Domba yang Disimpan pada Suhu Rendah (3-5ºC). Jakarta : JITV 11 (4): 287-294.

Surachman, M., Herdis, M. A. Setiadi, dan M. Rizal.,(2006) Cryopreservation of Ram Epididymal Spermatozoa Using Lecithin-Based Extender. Indonesia : Jurnal Indonesia Trop. Anim. Agric. 31 (2) : 83-89.

Weber, B.F.,



Archive Article

Cover Media Content

Volume : 3 / No. : 2 / Pub. : 2014-10
  1. Measurement Of Nuclear Dna Damage Of Simental Bull Spermatozoa Post Thawing With Sentrifuged Egg Yolk Skim And Soybean Lecitin Diluter
  2. Viability Of Spermatozoa Merino Sheep With Addition Of Various Nacl Concentration
  3. Numbers Resistance Spermatozoa Of Merino Rams And Etawa Goat In Nacl 0.9%, Nacl 1% And Nacl 1.1%
  4. Response To The Onset Of Estrous After Revocation Mpa (medroxy Progesterone Acetate) Intravaginal With Followed By Giving Gonadotropin Hormone On Fat Tailed Sheep
  5. Effect Of Pregnant Mare Serum Gonadotropin (pmsg) In Combination With Follicle Stimulating Hormone (fsh) For Superovulatory Treatment On The Onset And Duration Of Estrus In Fat Tailed Ewes (ovis Aries)
  6. The Effect Of Various Thawing Time On Frozen Semen Of Fat Tailed Sheep For Viability, Motility And Intact Plasma Membrane Of Spermatozoa
  7. Efforts To The Revenue Livestock Laying Duck And Fight Through The Surface Water Pollution Feed Management, Waste And Diversified Business
  8. Reproduction Efficiency Of Artificial Insemination Beef Cattle In East And West District, Kabupaten East Lombok On 2012
  9. Reproductive Efficiency Of Limousin And Simmental Cross Resulted From Artificial Insemination (ai) During 2012 In Ngoro, Jombang
  10. The Influence Of Mangosteen Peels Infusion (garcinia Mangostana L.) To Acrosome Reaction On Rats Spermatozoa
  11. Addition Of Cattle Seminal Plasma To Sperm Quality Goat On Freezing Process With Time Equilibration Different
  12. Identification Fertility Associated Antigen (faa) In Seminal Vesicle Of Brahman Bull Using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (sds-page) And Western Blot
  13. Isolation And Identification Of Osteopontin Sperm Membrane An Effort To Improve The Quality Of Holstein Fressian Dairy Bull Frozen Semen
  14. Suplementation Of Insuline-like Growth Factor-i Pregnant Thoroughbred Mare Serumon Increase Of Bovine Embrio Cleavage Rates

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