Detail Article


ISSN 2302-6464

Vol. 3 / No. 2 / Published : 2014-10

Order : 1, and page :225 - 230

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Original Article :

Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter

Author :

  1. Novia Candrawati*1
  2. Suherni Susilowati*2
  3. Bambang Poernomo S.R.*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The research was aimed to compare egg yolk skim and soybean lecithin diluter after sentrifugation. Comparison both diluter measured by nuclear DNA damage of Simental bull spermatozoa. All parameter were measured before and after sentrifugation. Apoptotic sperm nuclear damage was measured by TUNNEL assay metode using Apo-BrdU-IHCTM In Situ DNA Fragmentation Assay Kit.  The result of evaluation of nuclear DNA damage showed significant difference (p<0,05), it was perhaps caused by the composition of soybean lecithin diluter was more complete with the present of antioxidant. The result of spermatozoan quality measurement showed there were no significant difference except for the motility that showed significant difference before sentrifugation (p<0,05).

Keyword :

Diluents, centrifugation, sperm nuclear DNA,

References :

Darmawan, H. ,(2007) Production of ROS and its effect on mitochondrial and nuclear DNA, human spermatozoa, and sperm function. Indonesia : Media Jurnal Indonesia.16 (2):127-133.

Hadi, R.S.,(2011) Apoptosis Pada Sperma Sebagai Petanda Adanya Gangguan Kesuburan Pria. Indonesia : Majalah Kesehatan Pharma Medika. 3(2): 282-285.

Ilyas, S. ,(2007) Analysis of protein fas expression and caspase 3 activated at the suppression phase to sperm quantity by androgen/progestin combination. Sumatera : Jurnal Biologi Sumatera. 2 (2): 45 – 47.

Rizal, M., Maman, S., Herdis, dan Achmad, S.A.,(2006) Peranan Plasma Semen dalam Mempertahankan Kualitas Spermatozoa Asal Epididimis Domba yang Disimpan pada Suhu Rendah (3-5ºC). Jakarta : JITV 11 (4): 287-294.

Surachman, M., Herdis, M. A. Setiadi, dan M. Rizal.,(2006) Cryopreservation of Ram Epididymal Spermatozoa Using Lecithin-Based Extender. Indonesia : Jurnal Indonesia Trop. Anim. Agric. 31 (2) : 83-89.

Weber, B.F.,

Archive Article

Cover Media Content

Volume : 3 / No. : 2 / Pub. : 2014-10
  1. Measurement Of Nuclear Dna Damage Of Simental Bull Spermatozoa Post Thawing With Sentrifuged Egg Yolk Skim And Soybean Lecitin Diluter
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