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OVOZOA

ISSN 2302-6464

Vol. 3 / No. 1 / Published : 2014-04

TOC : 1, and page :145 - 149

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Original Article :

Morning and evening serum testosterone level of limousine crossbreed bulls

Author :

  1. Gamasy Aghani*1
  2. Laba Mahaputra*2
  3. Suryanie Sarudji*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The purpose of this research was to determine blood serum testosterone levels of Limousine crossbreed bulls in morning and evening, as an information to determine the best time of mating or semen collection for artificial insemination purposes. There were ten, two to three years old Limousin crossbreed bulls from which each 10 ml blood samples were collected through the jugular vein using disposible syringe. Blood sampling was performed twice, in the morning and evening. Blood serum was stored in a freezer with a temperature of -18oC until hormone assay performed. Hormone levels were analysed by ELISA (Enzyme Linked Immunosorbent Assay) technique. Statistical t-test was used to compare the testosterone levels. The result test of blood serum testosterone levels (means±SD) of Limousine crossbreed bull in the morning had an average of 6.88 ± 3.3 ng/ml and in the evening had an average of 2.8 ± 0.77 ng/ml. Statistical analysis showed very significant higher testosterone level in the morning componed to those in the evening (p<0.05)

Keyword :

Testosterone level, blood serum, Limousine crossbreed bull, ELISA,


References :

  1. Mahaputra, L. dan K. Soehadi, (1993). Kadar Testosteron Urine Pagi dan Siang Hari pada Pria Menikah dan Belum Menikah. Surabaya : Media IDI. 18. 26-28.
  2. Mathevon, M., M. Buhr and J.C.M. Dekkers, (1998). Environmental, Management and Genetic Factors Affecting Semen Production in Holstein Bulls. USA : J. Dairy Sci 81 :3321-3330
  3. Melson, B.E., J.L. Brown, H.M. Schoeman, G.L. Tarnavsky and J.J. Reeves, (1986). Evaluation of Testosteron during Chronic LHRH Argonist Treatment in the Bull. USA : J. Anim. Sci. 62:199-207


   


Archive Article

Cover Media Content

Volume : 3 / No. : 1 / Pub. : 2014-04
  1. Morning and evening serum testosterone level of limousine crossbreed bulls
  2. Test potential polyclonal antibodies local (abpo local pmsg) and patents (abpo patent pmsg) pmsg (pregnant mare serum gonadotropin) from male rabbit (oryctolagus cuniculus) to pregnancy mice (mus musculus)
  3. Dna fragmentation as apoptotic indicator and it relation with sperm viability and motility of domba ekor gemuk frozen semen in different thawing duration
  4. Determination encoding genes heat stable toxin (st) and protein of pathogens escherichia coli pili k99 was isolated from dairy cow feces using polymerase chain reaction (pcr)
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  13. Effect of equilibration time extender to post thawing motility and viability of merino sheep’s sperms in egg yolk skim diluents
  14. The effect of the combination progesterone in sponge intravaginal with equine chorionic gonadotropin (ecg) to the occurrence of estrous on fat tailed sheep