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OVOZOA

ISSN 2302-6464

Vol. 6 / No. 1 / Published : 2017-04

TOC : 15, and page :75 - 82

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Original Article :

Mutasi dan delesi pada genotip sapi madura akibat persilangan

Author :

  1. Dr. Budi Utomo, drh., M.Si.*1
  1. Dosen Fakultas Kedokteran Hewan

Abstract :

       Crossbreeding between Madura cattle with superior male female cattle other nations (exotic cattle) often occurs in some area. These crosses will have consequences that germplasm conservation cow Madura Madura Island will be no longer valid, except in Sapudi Island. According to FAO (2000) that, animal genetic resources native will tend to become extinct as a result of the new market demand (massive operations), uncontrolled crossing, turn breeds (replacement of the nation's cattle already exists with the nation's new cow) and the activities of agricultural mechanization (replacement of power use cow with engine power to cultivate farmland) .Sapi Madura breed (race) local cattle that formed as a result of natural insulation and environmental influences, so as to have uniformity characteristics that stand out among other local breeds of beef cattle in Indonesia. With the contribution of genetic traits zebu cattle as tolerant of stress due to climate and durability against ticks as well as natural selection and rigorous environment over a period of time, then a cow cattle Madura become a nation that has very high adaptability to the environment. Besides, Madura cattle have a good response to the improvement of feed and resistant to feed with a high content of crude fiber (Soehadji, 1993).        Benefits of using mtDNA according Duryadi (1994), among others (1) As genetic markers in intraspecific variability studies (inter population) that can provide information both qualitatively and quantitatively; (2) Can be used to track the relatively new events such as the study of natural hybridization between two subspecies; (3) Can be used for a historical reconstruction of genealogy matrilinier a species or between existing populations; (4) to reconstruct phyilogenic of several species that are close together. This study uses the D-loop region fragment Madura cattle to get the data so that it can determine the genetic diversity between individual nucleotide composition of Madura cattle and identify its phenotypes. Such data can be used to identify the genetic purity of Madura cattle that exist in the area of Sapudi island, Sumenep, Pamekasan Pamekasan, Sampang, Bangkalan.                  The sequence analysis of mtDNA D-loop region found 50 sites of genetic diversity that is Madura cattle from Pamekasan substitution mutation, there were 15 transitions, 9 transversions substitution mutation. Sampang Madura cattle have 13 substitution mutations transitions, 8 transversions substitution mutation. Madura cattle from Sumenep have 2substitution transitions mutation, 2 transversions substitution mutations and 1 deletion. Despite this high level of variation in this study did not result in a change in phenotype as D-Loop region is non-coding regions that are not expressed.

Keyword :

Germplasm, National Beef, Genetic Characters, mtDNA, Genetic Purity,


References :

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Archive Article

Cover Media Content

Volume : 6 / No. : 1 / Pub. : 2017-04
  1. Conception rate (cr) dan service per conception (s/c) pada sapi peranakan friesian holstein akseptor inseminasi buatan di kud karangploso kabupaten malang
  2. Penerapan chaid sebagai alat pengklasifikasi : studi kasus tanggap peternak pada pengabdian masyarakat tentang inseminasi buatan pada sapi madrasin di desa sembilangan kabupaten bangkalan madura
  3. Pengaruh penambahan glukosa sebagai sumber energi terhadap viabilitas dan motilitas spermatozoa sapi madura dalam pengencer susu skim kuning telur
  4. Pengaruh pemberian ekstrak buah terong ungu (solanum melongena l.) pada mencit (mus musculus) terhadap angka kebuntingan
  5. Conception rate dan service per conception pada sapi potong hasil inseminasi buatan di kecamatan sungai raya dan kecamatan teluk pakedai kabupaten kubu raya provinsi kalimantan barat tahun 2014
  6. Gambaran histopatologi epididimis kelinci (oryctolagus cuniculus) setelah dilakukan imunisasi dengan vaksin whole bakteri brucella abortus strain 19
  7. Pola resistensi antibiotik β-laktam pada escherichia coli yang diisolasi dari susu di peternakan sapi perah surabaya
  8. Pola resistensi antibiotik non beta laktam pada escherichia coli yang diisolasi dari susu di peternakan sapi perah surabaya
  9. Perbandingan kualitas susu antara pasar dan kud argopuro krucil, probolinggo
  10. Pengaruh pemberian insulin-like growth factor-i (igf -i) dari serum kuda crossbreed bunting terhadap siklus estrus mencit (mus musculus)
  11. Pengaruh penambahan plasma seminalis sapi simmental terhadap motilitas dan viabilitas spermatozoa domba ekor gemuk setelah ekuilibrasi pada proses pembekuan
  12. Pengaruh musim terhadap conception rate dan service per conception sapi potong akseptor inseminasi buatan di kabupaten nganjuk
  13. Efisiensi reproduksi dan indeks fertilitas sapi perah friesian holstein hasil inseminasi buatan pada berbagai paritas dan umur di wilayah kud tani wilis, sendang, tulungagung periode tahun 2014
  14. Pengaruh pemberian serbuk buah terong ungu (solanum melongena l) terhadap proses spermatocytogenesis testis tikus putih (rattus norvegicus) pasca pemberian diet tinggi lemak
  15. Mutasi dan delesi pada genotip sapi madura akibat persilangan