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OVOZOA

ISSN 2302-6464

Vol. 5 / No. 2 / Published : 2016-10

TOC : 3, and page :82 - 86

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Original Article :

Penambahan l-arginin dalam pengencer susu skim kuning telur terhadap viabilitas dan motilitas spermatozoa sapi limousin post thawing pada semen beku

Author :

  1. )Islakhul Aila*1
  2. Prof. Dr. Nunuk Dyah Retno L*2
  3. Dr. Erma Safitri, drh., M.Si.*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

This research was aimed to determine of adding L-arginin in viability and motility limousin bull’s sperm on post thawing examination in skim milk-egg yolk extender. This research used fresh samples of limousine bull’s semen collected by using artificial vagina, then were devided into 4 treatments, adding L-arginin 0,004 M (P1),  adding L-arginin 0,005 M (P2), adding L-arginin 0,006 M (P3) and without adding L-arginin (P0). The experimental design that used was Complete Randomized Design (CRD). Analysis of the data using Analysis of Variant (ANOVA) One Way then proceed to the duncan to determine significant differences between treatments. Persentage viability showed significant differences between P3 to P0, P1 and P3, but P0 to P1 and P0 to P2 no real difference. Persentage motility showed significant differences between P3 to P0, P1 and P3, but between P0, P1 and P2, there no real difference. Results showed that adding L-arginin 0,006 M in skim milk-egg yolk extender as the best consentration to increase viability and motility  limousin bull semen post thawing with significant (P < 0.05) differences when compared with the control and consentration of L-arginin 0,004 and 0,005 M

Keyword :

Viability, Motility, Limousin bull sperm, L-arginin, Skim milk-egg yolk extender. ,


References :

  1. Dwiyanto, K., (2008). Pemanfaatan Sumber Daya Lokal dan Inovasi Teknologi dalam Mendukung Pengembangan Sapi Potong di Indonesia.. I(3): 173-188. : Pengembangan Inovasi Pertanian.
  2. Griveau, J.F., E. Dumont., P. Renard., J.P. Callegari and D. Le Lannou., (1995). Reactive Oxygen Species Lipid Peroxidation and Enzymatic Defense System in Human Spermatozoa.. 103: 17-26. : Journal Reproduction Fertility.
  3. O’Flaherty, C., P. Rodriguez and S. Srivastava., (2004). L-Arginine promotes Capacitation and Acrosome Reaction in Cryopreserved Bovine Spermatozoa. . 1674: 215– 221. : Biochimica et Biophysica Acta.
  4. Srivastava, S., P. Desai., E. Coutinho and G. Govil., (2006). Mechanism of Action of L-arginine on The Vitality of Spermatozoa is Primarily Through Increased Biosynthesis of Nitric Oxide.. 74(5): 954-958. : Biology of Reproduction.


   


Archive Article

Cover Media Content

Volume : 5 / No. : 2 / Pub. : 2016-10
  1. Efektifitas hormon prostaglandin f2α (pgf2α) dan hormon gonadotropin terhadap gambaran ovarium dankadar progesteron tikus putih (rattus norvegicus) dalam teknik sinkronisasi birahi dan superovulasi
  2. Pengaruh penambahan perasan buah labu kuning (cucurbita moschata) dalam diluter terhadap integritas membran plasma spermatozoa sapi simmental post thawing
  3. Penambahan l-arginin dalam pengencer susu skim kuning telur terhadap viabilitas dan motilitas spermatozoa sapi limousin post thawing pada semen beku
  4. Pengaruhpemberian pasta tomat (solanum lycopersicum) terhadap jumlah sel spermatogonium pada testis mencit (mus musculus) yang dipapar boraks
  5. Persentase membran plasma utuh dan nekrosis spermatozoa sapi limousin post thawing setelah penambahan l-arginin berbagai konsentrasi dalam pengencer skim kuning telur
  6. Pengaruh pemberian ekstrak daging buah mahkota dewa (phaleria macrocarpa) terhadap kualitas spermatozoa tikus putih (rattus norvegicus)
  7. Hubungan paritas dan umur terhadap indeks fertilitas sapi perah peranakan frisian holstein (pfh) di koperasi unit desa (kud) sri wigati pagerwojo tulungagung
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  10. Pengaruh temperatur rektum dan temperatur vagina saat inseminasi buatan terhadap angka kebuntingan pada sapi perah di dataran tinggi dan dataran rendah
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  13. Gambaran histopatologi tubulus seminiferus mencit (mus musculus) yang terpapar brucella suis
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