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Detail Article

OVOZOA

ISSN 2302-6464

Vol. 4 / No. 1 / Published : 2015-04

TOC : 5, and page :18 - 23

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Original Article :

Pengaruh pemberian infusa kulit manggis (garcinia mangostana l.) terhadap motilitas, viabilitas dan integritas membran spermatozoa tikus putih (rattus norvegicus)

Author :

  1. Dhonna Mardiana*1
  2. Dr. Budi Utomo, drh., M.Si.*2
  3. Dr. Mufasirin, drh., M.Si.*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The purpose of this research was to know the effect on mangosteen peel infuse for spermatozoa quality including the motility, viability and membrane integrity of spermatozoa. The method used for preparing the mangosteen peel infuse in this research was done by cutting the mangosteen peel in to small pieces then dried in the oven at 45°C, after that make it smoothed using a dry blender and finally boiled it at 90°C for 15 minutes. The giving administration of mangosteen peel infusion in mice was done orally as much as 2 ml appropriate with rat gastric content using 5 ml syringe attached with with canula. The consentration of mangosteen peel was 5 %, 10 % and 15 % and used vitamin C for the positive control also aquabides for the negative control. This research result was analyzed with ANOVA followed by Tukey test (p < 0.05). The result showed that there was no significant different level between the negative and positive control group with 5% of mangosteen peel infusion level. There had very significant difference level for 10% and 15% of mangosteen peel infusion level and control group. So, the optimum consentration was 10% and the maximum consentration was 15 % of the mangosteen peel infusion level.

Keyword :

Spermatozoa, mangosteen peel infuse, motility , viability , membrane integrity,


References :

  1. Axner, E., C. L. Forsberg and S. Einarsson., (2000). Morphology and motility of spermatzoa from different region of the peididymal duct in the domestic cat. . 45: 767 – 777. : Theriogenology
  2. Fonseca, JF. CA. Torres. VV. Maffili. AM. Borges. ADF. Santos. RFM Oliveira. , (2005). The Hypoosmotic Swelling Test in Fresh Goat Spermatozoa.. 2 : 139-144 : J. Animal Reproduction.
  3. Ho, C.K. Y.L, Huang. C.C, Chen., (2002). Garcinone E, a xanthone derivative, has potent cytotoxic effect against hepatocellular carcinoma cell lines, . 68(11):975-979. : Planta Med.


   


Archive Article

Cover Media Content

Volume : 4 / No. : 1 / Pub. : 2015-04
  1. Boraks mengakibatkan penurunan jumlah sel spermatogonium dan sel sertoli pada gambaran histopatologi testis tikus putih (rattus norvegicus)
  2. Kadar progesteron serum domba ekor gemuk sebelum pemasangan dan sesudah pencabutan implan progesteron subkutan
  3. Pemberian injeksi kombinasi hormon pmsg dan hcg terhadap waktu timbulnya birahi pada sapi madura di desa sembilangan kecamatan bangkalan kabupaten bangkalan
  4. Pengaruh pemberian infusa kulit manggis (garcinia mangostana l.) terhadap peningkatan persentase kapasitasi spermatozoa tikus putih (rattus norvegicus)
  5. Pengaruh pemberian infusa kulit manggis (garcinia mangostana l.) terhadap motilitas, viabilitas dan integritas membran spermatozoa tikus putih (rattus norvegicus)
  6. Perubahan histopatologi jaringan interstitial testis tikus putih (rattus norvegicus) yang dipapar boraks (na2b4o7.10h2o)
  7. Waktu inseminasi buatan pada sapi perah yang digertak birahi dengan hormon prostaglandin f2α dan hormon gonadotropin
  8. Isolasi dan karakterisasi fertility associated antigen (faa) dalam membran spermatozoa sebagai bahan aktif kesuburan spermatozoa sapi
  9. Perbedaan non return rate (nrr) hari ke 21 dan conception rate (cr) hari ke 75 sapi perah yang disinkronisasi birahi menggunakan prostaglandin f2α (pgf2α) dengan gonadotropin (kombinasi pmsg dan hcg)
  10. Pengujian kualitas semen segar kambing gembrong untuk proses pembekuan di teaching farm
  11. Pengaruh waktu equilibrasi terhadap motilitas dan viabilitas spermatozoa kambing gembrong post thawing dalam pengencer skim kuning telur
  12. Nilai bod (biochemical oxygen deman) dan cod (chemical oxygen deman) air kolam dan limbah usaha perikanan yang menggunakan kotoran bebek sebagai media budidaya ikan lele
  13. Pemeriksaan kadar hormon estrogen sebagai indikator diagnostik adanya kebuntingan dini pada kuda poni
  14. Pengaruh berbagai konsentrasi kuning telur itik dalam susu skim sebagai pengencer semen domba ekor gemuk terhadap motilitas, viabilitas dan keutuhan membran plasma spermatozoa before freezing