UNIVERSITAS AIRLANGGA



Detail Article

OVOZOA

ISSN 2302-6464

Vol. 4 / No. 1 / Published : 2015-04

Order : 4, and page :14 - 17

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Original Article :

Pengaruh pemberian infusa kulit manggis (garcinia mangostana l.) terhadap peningkatan persentase kapasitasi spermatozoa tikus putih (rattus norvegicus)

Author :

  1. Veny Putri Lestari*1
  2. Prof. Dr. Sarmanu, drh., MS.*2
  3. Dr. Budi Utomo, drh., M.Si.*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The aims of this research was to know the effect of mangosteen (Garcinia mangostana L) peel infusion on increasing the capacitation percentage on spermatozoa of white rats (Rattus norvegicus). The inspection of semen feasibility was done for further treatment. The treatment were devided into five groups with 5 rats in each group. The treatments are rats that were exposed in sunlight for 15 minutes and was given aquabides (P0), rats that were exposed in sunlight for 15 minutes and was given vitamin C (P1), rats that were exposed in sunlight for 15 minutes and was given mangosteen peel infusion 5 % (P2), rats that were exposed in sunlight for 15 minutes and was given mangosteen peel infusion 10 % (P3), and rats that were exposed in sunlight for 15 minutes and was given mangosteen peel infusion 15 % (P4). Rats were given adaptation time for 1 week, then be given treatment for 2 weeks. In the end of treatment, semen of rats were taken from the epididimis tract, then the capacitation spermatozoa was checked by reagent FITC (Flourescent Isotiocianat). Data were compared using Kruskal-Wallis test and Mann Whitney. The result showed that on P4 have the highest capacitation presentage (21,20) that not significantly difference with P3 (17,00), whereas P0 have the lowest effect (4,80) and P1 and P2 have same capacitation presentage (11,00). The result show that mangosteen peel infusion 10 % and 15 % can increase capacitation presentage on white rats (Rattus norvegicus).

Keyword :

White rats, Mangosteen peel infusion, Capatitation presentage,


References :

Aitken RJ, Clarkson JS. ,(1987) Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. 81:459–469 : J Reprod Fertil.

Makker K, Agarwal A, Sharma R. ,(2009) Oxidative stress and male infertility. 129: 357 – 67 : Indian J Med Res

Sanocka,D. and M.Kurpisz.,(2004) Reactive oxygen species and sperm cells. 2 : 12 : Reprod. Biol. Endocrinol.

Sikka,S.C. ,(2004) Role of oxidative stress and antioxidants in andrology and assisted reproductive technology. 25: 5-18. : J. Androl.

Weecharangsan W, Opanasopit P, Sukma M, Ngawhirunpat T, Sotanaphun U, Siripong P.,(2006) Antioxidative and neuroprotective activities of extracts from the fruit hull of mangosteen (Garcinia mangostana Linn.) 15(4):281-287. : Med Princ Pract.





Archive Article

Cover Media Content

Volume : 4 / No. : 1 / Pub. : 2015-04
  1. Boraks Mengakibatkan Penurunan Jumlah Sel Spermatogonium Dan Sel Sertoli Pada Gambaran Histopatologi Testis Tikus Putih (rattus Norvegicus)
  2. Kadar Progesteron Serum Domba Ekor Gemuk Sebelum Pemasangan Dan Sesudah Pencabutan Implan Progesteron Subkutan
  3. Pemberian Injeksi Kombinasi Hormon Pmsg Dan Hcg Terhadap Waktu Timbulnya Birahi Pada Sapi Madura Di Desa Sembilangan Kecamatan Bangkalan Kabupaten Bangkalan
  4. Pengaruh Pemberian Infusa Kulit Manggis (garcinia Mangostana L.) Terhadap Peningkatan Persentase Kapasitasi Spermatozoa Tikus Putih (rattus Norvegicus)
  5. Pengaruh Pemberian Infusa Kulit Manggis (garcinia Mangostana L.) Terhadap Motilitas, Viabilitas Dan Integritas Membran Spermatozoa Tikus Putih (rattus Norvegicus)
  6. Perubahan Histopatologi Jaringan Interstitial Testis Tikus Putih (rattus Norvegicus) Yang Dipapar Boraks (na2b4o7.10h2o)
  7. Waktu Inseminasi Buatan Pada Sapi Perah Yang Digertak Birahi Dengan Hormon Prostaglandin F2α Dan Hormon Gonadotropin
  8. Isolasi Dan Karakterisasi Fertility Associated Antigen (faa) Dalam Membran Spermatozoa Sebagai Bahan Aktif Kesuburan Spermatozoa Sapi
  9. Perbedaan Non Return Rate (nrr) Hari Ke 21 Dan Conception Rate (cr) Hari Ke 75 Sapi Perah Yang Disinkronisasi Birahi Menggunakan Prostaglandin F2α (pgf2α) Dengan Gonadotropin (kombinasi Pmsg Dan Hcg)
  10. Pengujian Kualitas Semen Segar Kambing Gembrong Untuk Proses Pembekuan Di Teaching Farm
  11. Pengaruh Waktu Equilibrasi Terhadap Motilitas Dan Viabilitas Spermatozoa Kambing Gembrong Post Thawing Dalam Pengencer Skim Kuning Telur
  12. Nilai Bod (biochemical Oxygen Deman) Dan Cod (chemical Oxygen Deman) Air Kolam Dan Limbah Usaha Perikanan Yang Menggunakan Kotoran Bebek Sebagai Media Budidaya Ikan Lele
  13. Pemeriksaan Kadar Hormon Estrogen Sebagai Indikator Diagnostik Adanya Kebuntingan Dini Pada Kuda Poni
  14. Pengaruh Berbagai Konsentrasi Kuning Telur Itik Dalam Susu Skim Sebagai Pengencer Semen Domba Ekor Gemuk Terhadap Motilitas, Viabilitas Dan Keutuhan Membran Plasma Spermatozoa Before Freezing