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OVOZOA

ISSN 2302-6464

Vol. 6 / No. 1 / Published : 2017-04

TOC : 10, and page :45 - 49

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Original Article :

Pengaruh pemberian insulin-like growth factor-i (igf -i) dari serum kuda crossbreed bunting terhadap siklus estrus mencit (mus musculus)

Author :

  1. Putri Sulisdiani*1
  2. Dr. Tjuk Imam Restiadi, drh., M.Si.*2
  3. Roesno Darsono, drh., M.Vet.*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The purpose of the research was to determine the effect of Insulin-Like Growth Factor-I (IGF-I) derived from pregnant crossbreed mare serum (PMS) on estrous cycle in mice (Mus musculus). The subject of this research were 35 female mice. The research was arranged by Completely Randomized Design (CRD) with seven treatment and five replications. The treatment were K0 = 10 ng/ml of physiological NaCl, P11 = 10 ng/ml of IGF-I PMS, P12 = 20 ng/ml of IGF-I PMS, P13 = 40 ng/ml of IGF-I PMS, P21 = 10 ng/ml of IGF-I recombinant mouse, P22 = 20 ng/ml of IGF-I recombinant mouse, and P23 = 40 ng/ml of IGF-I recombinant mouse. Observed variables include estrous cycle phases that proestrus, estrus, metestrus, and diestrus phase. The data were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS did not significantly affect (p>0,05) on proestrus, metestrus, and diestrus phase. The addition of IGF-I PMS 40 ng/ml was extended (p<0,05) the estrus phase. It showed that did not significantly difference (p>0,05) between the effect of IGF-I PMS and IGF-I recombinant mouse against the mice estrous cycles.

Keyword :

IGF-I, IGF-I pregnant crossbreed mare serum, estrous cycle, Mus musculus,


References :

  1. Inoue, Ayako., Takeuchi, Sakae, and T. Sumio., (2005). Insulin-Like Growth Factor-I Stimulated DNA Replication in Mouse Endometrial Stroma Cells.. 51 : 3. : Journal of Reproduction and Development.
  2. Nalley, W.M.M., Handarini, R., Rizal, M., Arifiantini, R.I., Yusuf T.L., dan B. Purwantara., (2011). Determination of The Estrous Cycle Based on Vaginal Cytology and Hormone Profile in Timor Hind.. 12(2) : 98-106. : Jurnal Veteriner.
  3. Pine, MD., Hiney, J.K., Dearth, R.K., Brattonk, G.R. and Dees WL., (2005). IGF-I Administration to Prepubertal Female Rats Can Overcome Delayed Puberty Caused by Maternal Pb Exposure.. 21 (1) : 104 - 109. : Reprod Toxicol.
  4. Sutasurya, L.A., Sitasiwi, A.J and Ratnawati K., (2001). Penentuan Kandungan Estradiol (E2) dan Luteinizing Hormone (LH) pada Petaurus Breviceps Papuanus (Marsupialia) Selama Satu Siklus Estrus.. 11 : 25-35. : Jurnal Sains dan Teknologi Nuklir Indonesia.


   


Archive Article

Cover Media Content

Volume : 6 / No. : 1 / Pub. : 2017-04
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