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ISSN 2302-6464

Vol. 3 / No. 1 / Published : 2014-04

TOC : 12, and page :206 - 214

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Original Article :

Profile of progesterone and insulin-like growth factor-i (igf-i) in pregnant crossbred mare serum

Author :

  1. Tjuk Imam Restiadi*1
  2. Imam Mustofa*2
  3. Suzanita Utama*3
  1. Dosen Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The aim of the present study was the determation in pregnant crossbred mare by detection of progesteron and insulin-like growth factor-I (IGF-I) in serum by the technique of enzyme-linked immunosorbent assay (ELISA) sandwich. Fluctuation of progesterone levels in serum is highly correlated with changes in reproductive status of the mare, in order to ensure the establishment of pregnancy. Whereas in insulin-like growth factor-I is basically characterized as peptides secreted by liver and circulated in blood is bioactive material studied from pregnant crossbred mare serum. The results of progesterone levels from crossbred mare pregnancy that range between 1.8 ng/ml to 17.3 ng/ml, and IGF-I levels that range between 98 ng/ml to 654 ng/ml. Pregnancy test based on progesterone levels in crossbred mare serum are generally used only when palpation and ultrasound per rectum is not possible.

Keyword :

mare serum, crossbred, ELISA, progesterone, IGF-I,

References :

  1. Berg EL, McNamara DL and Keisler DH. , (2007). Endocrine Profiles of Peripar-turient Mares and Their Foals. In: Animal Growth, Physiology, and Reproduction.. USA : Anim Sci 85: 1660-1668.
  2. Derar RI, Haramaki S, Hoque SMD, Hashi-zume T, Osawa T, Taya K, Watanabe G, and Miyake YI. , (2006). Immuno-reactive Insulin-Like Growth Factor in Plasma during Pre and Post-Partum Periods of Thoroughbred Mares from Which The Newborn were Removed: Its Pattern, Physiological Function and Relation to Other Hormones. . USA : Equine Sci. 17(3): 75-79.
  3. De Kock SS, Rodgers JP, Swanepoel BC, and Guthrie AJ. , (2001). Administration of Bovine, Porcine and Equine Growth Hormone to The Horse: Effect on Insulin-Like Growth Factor-I and Selected IGF Binding Proteins. . USA : Journal of Endocrinology 171: 163-171
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  6. Lejeune J.-Ph, Franck T, Gang M, Schneider N, Michaux C, Deby-Du-pont G, and Serteyn D. , (2007). Plasma Concentration of Insulin-Like Growth Factor I (IGF-I) in Growing Ardenner Horses Suffering from Juvenile Digital Degenerative Osteoarthropathy. . USA : Veterinary Research Communications 31: 185-195.


Archive Article

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Volume : 3 / No. : 1 / Pub. : 2014-04
  1. Morning and evening serum testosterone level of limousine crossbreed bulls
  2. Test potential polyclonal antibodies local (abpo local pmsg) and patents (abpo patent pmsg) pmsg (pregnant mare serum gonadotropin) from male rabbit (oryctolagus cuniculus) to pregnancy mice (mus musculus)
  3. Dna fragmentation as apoptotic indicator and it relation with sperm viability and motility of domba ekor gemuk frozen semen in different thawing duration
  4. Determination encoding genes heat stable toxin (st) and protein of pathogens escherichia coli pili k99 was isolated from dairy cow feces using polymerase chain reaction (pcr)
  5. Biopotensi pmsg (pregnant mare serum gonadotrophin) local horse to speed time arising estrus, number of follicles, corpus luteum, progesterone levels and pregnancy rate in madura cattle
  6. Potential of vitamin e (α-tocopherol) of malondialdehyde (mda) levels testis mice (mus muscular) induced 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (tcdd)
  7. Effect of “sarang semut” (myrmecodia pendans) on the number of spermatogenic cells in seminiferous tubules of mice (mus musculus) with excessive physical treatment
  8. The effect of estrous syncronization by pgf2α and superovulation by pmsg and hcg and artificial insemination on heat response, pregnancy rates, litter size and sex ratio of the crossbread etawa goat
  9. Examination of fresh semen quality of merino sheep for frozen semen production in teaching farm of veterinary medicine faculty of airlangga university
  10. Identification of sperm morphometry in merino sheep and fat-tailed sheep
  11. The correlation between body weight with body lenght, wither’s height, heart girth, scrotal circumference, volume, and semen quality of peranakan etawa goat
  12. Profile of progesterone and insulin-like growth factor-i (igf-i) in pregnant crossbred mare serum
  13. Effect of equilibration time extender to post thawing motility and viability of merino sheep’s sperms in egg yolk skim diluents
  14. The effect of the combination progesterone in sponge intravaginal with equine chorionic gonadotropin (ecg) to the occurrence of estrous on fat tailed sheep