Detail Article


ISSN 2302-6464

Vol. 3 / No. 2 / Published : 2014-10

Order : 14, and page :294 - 304

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Original Article :

Suplementation of insuline-like growth factor-i pregnant thoroughbred mare serumon increase of bovine embrio cleavage rates

Author :

  1. Tjuk Imam Restiadi*1
  1. Dosen Fakultas Kedokteran Hewan

Abstract :

In vitro fertilization (IVF) is a technique that is expected to provide superior livestock mass production, but the problem is that the materials needed still have to be imported at a high price as well. Optimization of IVF technique can be implemented by supplementing materials which are cheap and easily available in the country. Indonesia has a national racehorse, the thoroughbred. In pregnant mare serum there is insulin-like growth factor-I (IGF-I) which is expected to be utilized to improve the outcome of in vitro fertilization. This study aimed to verify the effect of pregnant thoroughbred mare serum derived IGF-I supplementation into in vitro fertilization media on the increase of oocyte maturation and embryo cleavage rates. Oocyte maturation with the supplementation of IGF-I eluate were compared to those of the patent IGF-I, each with a concentration of 5, 10 and 20 ng/ml as well as to those of the negative control (oocyte maturation without supplementation) and the positive control (oocyte maturation supplemented with 15 IU pregnant mare serum gonadotropin (PMSG). Matured oocytes were then inseminated with frozen semen of elite bull. The observed variables were the percentage of cleaved embryos at 48 hours after fertilization. Results of IVF showed that the percentage embryo cleavage were not significantly different (p >0.05) between group supplemented with IGF-I eluate, patent IGF-I and the positive control (PMSG), but were significantly different (p <0.05) compared to the negative control. Conclusion: pregnant thoroughbred mare serum IGF-I eluate increased embryo cleavage rates.

Keyword :

Insulin-like growth factor-I, thoroughbred mare serum, cleavage, cow oocytes,

References :

Farag, IM., Girgis, SM., Khalil, WKB., Hassan, NHA., Sakr, AAM., Abd Allah, SM., and Ali, NI. ,(2009) Effect of Hormones, Culture Media and Oocyte Quality on In Vitro Maturation of Egyptian Sheep Oocytes. USA : Journal of Applied Biosciences 24: 1520 – 1534.

Machatkova, M., Krausova, K., Jokesova, E., and Tomanek, M. ,(2004) Developmental Competence of Bovine Oocytes: Effects of Follicle Size and The Phase of Follicular Wave on In Vitro Embryo Production. USA : Theriogenology 61: 329–335.

Neira, JA., Tainturier, D., Pen˜a, MA., Martal, J. ,(2010) Effect of The Association of IGF-I, IGF-II, bFGF, TGF-b1, GM-CSF, and LIF on The Development of Bovine Embryos Produced In Vitro. USA : Theriogenology 73: 595–604.

Shabankareh, HK., and Zandi, M. ,(2010) Developmental Potential of Sheep Oocytes Cultured in Different Maturation Media: Effects of Epidermal Growth Factor, Insulin-Like Growth Factor I, and Cysteamine. USA : Fertility and Sterility 94(1): 335-340.

Archive Article

Cover Media Content

Volume : 3 / No. : 2 / Pub. : 2014-10
  1. Measurement Of Nuclear Dna Damage Of Simental Bull Spermatozoa Post Thawing With Sentrifuged Egg Yolk Skim And Soybean Lecitin Diluter
  2. Viability Of Spermatozoa Merino Sheep With Addition Of Various Nacl Concentration
  3. Numbers Resistance Spermatozoa Of Merino Rams And Etawa Goat In Nacl 0.9%, Nacl 1% And Nacl 1.1%
  4. Response To The Onset Of Estrous After Revocation Mpa (medroxy Progesterone Acetate) Intravaginal With Followed By Giving Gonadotropin Hormone On Fat Tailed Sheep
  5. Effect Of Pregnant Mare Serum Gonadotropin (pmsg) In Combination With Follicle Stimulating Hormone (fsh) For Superovulatory Treatment On The Onset And Duration Of Estrus In Fat Tailed Ewes (ovis Aries)
  6. The Effect Of Various Thawing Time On Frozen Semen Of Fat Tailed Sheep For Viability, Motility And Intact Plasma Membrane Of Spermatozoa
  7. Efforts To The Revenue Livestock Laying Duck And Fight Through The Surface Water Pollution Feed Management, Waste And Diversified Business
  8. Reproduction Efficiency Of Artificial Insemination Beef Cattle In East And West District, Kabupaten East Lombok On 2012
  9. Reproductive Efficiency Of Limousin And Simmental Cross Resulted From Artificial Insemination (ai) During 2012 In Ngoro, Jombang
  10. The Influence Of Mangosteen Peels Infusion (garcinia Mangostana L.) To Acrosome Reaction On Rats Spermatozoa
  11. Addition Of Cattle Seminal Plasma To Sperm Quality Goat On Freezing Process With Time Equilibration Different
  12. Identification Fertility Associated Antigen (faa) In Seminal Vesicle Of Brahman Bull Using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (sds-page) And Western Blot
  13. Isolation And Identification Of Osteopontin Sperm Membrane An Effort To Improve The Quality Of Holstein Fressian Dairy Bull Frozen Semen
  14. Suplementation Of Insuline-like Growth Factor-i Pregnant Thoroughbred Mare Serumon Increase Of Bovine Embrio Cleavage Rates