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OVOZOA

ISSN 2302-6464

Vol. 3 / No. 2 / Published : 2014-10

TOC : 6, and page :247 - 251

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Original Article :

The effect of various thawing time on frozen semen of fat tailed sheep for viability, motility and intact plasma membrane of spermatozoa

Author :

  1. Wong Aihuwa Diah Lestari*1
  2. Suherni Susilowati*2
  3. Sunaryo Hadi Warsito*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The research was aimed to determine the effect and the best thawing time on frezen semen of fat-tailed sheep for viability, motility and intact plasma membrane spermatozoa. This research was conducted at the Teaching Farm Airlangga University, with type of research is true experiment in a Completely Randomized Design (CRD). Each group consisted of 3 treatments and 6 replication. The sample in this study were frozen semen of fat-tailed sheep. The sample criteria used was fat-tailed sheep among 4 years old male, with weight 50 kg. The independent variable in this study was the thawing time of the frozen semen of fat-tailed sheep containing thawing time P1 = 15 seconds, P2 = 30 seconds, P3 = 45 seconds. The dependent variable was the quality of fat-tailed sheep’s spermatozoa. Then the data result processed using ANOVA oneway and continued with Honestly Significant Difference (HSD) test. Based on the result, it’s indicated that the difference thawing time showed a tangible influence againts (p<0,05) againts the percentage of viability, motility and intact plasma membrane. Highest average percentage obtained by 52,5% for viability, 33,3% for motility and 44,5% for intact plasma membrane on P2 treatment and lowest percentage obtained from P1 treatment with 36,8% for viability, 17,5% for motility and 33,3% for intact plasma membrane.

Keyword :

Frozen semen, Thawing, Viability, Motility, Intact Plasma Membrane,


References :

  1. Lechniak. M. Sabrani and W Farstad. , (2002). Effect of Thaw Rates on Survival of Buffalo Spermatozoa Frozen In Straws.. USA : Journal of Dairy Science., 67: 1523-1538.


   


Archive Article

Cover Media Content

Volume : 3 / No. : 2 / Pub. : 2014-10
  1. Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter
  2. Viability of spermatozoa merino sheep with addition of various nacl concentration
  3. Numbers resistance spermatozoa of merino rams and etawa goat in nacl 0.9%, nacl 1% and nacl 1.1%
  4. Response to the onset of estrous after revocation mpa (medroxy progesterone acetate) intravaginal with followed by giving gonadotropin hormone on fat tailed sheep
  5. Effect of pregnant mare serum gonadotropin (pmsg) in combination with follicle stimulating hormone (fsh) for superovulatory treatment on the onset and duration of estrus in fat tailed ewes (ovis aries)
  6. The effect of various thawing time on frozen semen of fat tailed sheep for viability, motility and intact plasma membrane of spermatozoa
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  8. Reproduction efficiency of artificial insemination beef cattle in east and west district, kabupaten east lombok on 2012
  9. Reproductive efficiency of limousin and simmental cross resulted from artificial insemination (ai) during 2012 in ngoro, jombang
  10. The influence of mangosteen peels infusion (garcinia mangostana l.) to acrosome reaction on rats spermatozoa
  11. Addition of cattle seminal plasma to sperm quality goat on freezing process with time equilibration different
  12. Identification fertility associated antigen (faa) in seminal vesicle of brahman bull using sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) and western blot
  13. Isolation and identification of osteopontin sperm membrane an effort to improve the quality of holstein fressian dairy bull frozen semen
  14. Suplementation of insuline-like growth factor-i pregnant thoroughbred mare serumon increase of bovine embrio cleavage rates