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OVOZOA

ISSN 2302-6464

Vol. 3 / No. 2 / Published : 2014-10

TOC : 10, and page :270 - 273

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Original Article :

The influence of mangosteen peels infusion (garcinia mangostana l.) to acrosome reaction on rats spermatozoa

Author :

  1. Gian Robby F. A.*1
  2. Budi Utomo*2
  3. Sri Hidanah*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The given of mangosteen peels infusion had an aim to determine the spermatozoa qualities on acrosome reaction of spermatozoa. The method used for prepairing the mangosteen peels infusion was begun by made small cut on the peels, then drying it at 45°C than smoothed the peels. The dried peels boiled at 90°C for 15 minutes. Two ml infusion was given to the rats by using sonde according to the rats gastric dose. The sonde made for 5 ml syringe modificated with canula. The dose given on rats were 5%, 10% and 15% also vitamin C as positive control and the control was given with aquabidest. The data was analyzed using ANOVA and continued with Tukey method (P > 0,05). The result showed that there were no significant differences between control, positive control and treatment with 5% dose on mangosteen peels infusion. But, another result showed that there were significant differences in control, 10% and 15% dose treatment on mangosteen peels. The optimum dose for giving the mangosteen peels infusion in this research was 10%, and the maximum dose was 15%.

Keyword :

Rats, Spermatozoa, mangosteen peels infusion, Acrosome reaction,


References :

  1. Aitken RJ, Clarkson JS. , (1987). Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. . USA : J Reprod Fertil. 81:459–469
  2. Rizal, M dan Herdis., (2008). Inseminasi Buatan pada Domba.. Jakarta. : Penerbit Rineka Cipta.
  3. Sikka, S.C. , (2004). Role of oxidative stress and antioxidants in andrology and assisted reproductive technology. . USA : J. Androl. 25:5-18.


   


Archive Article

Cover Media Content

Volume : 3 / No. : 2 / Pub. : 2014-10
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  2. Viability of spermatozoa merino sheep with addition of various nacl concentration
  3. Numbers resistance spermatozoa of merino rams and etawa goat in nacl 0.9%, nacl 1% and nacl 1.1%
  4. Response to the onset of estrous after revocation mpa (medroxy progesterone acetate) intravaginal with followed by giving gonadotropin hormone on fat tailed sheep
  5. Effect of pregnant mare serum gonadotropin (pmsg) in combination with follicle stimulating hormone (fsh) for superovulatory treatment on the onset and duration of estrus in fat tailed ewes (ovis aries)
  6. The effect of various thawing time on frozen semen of fat tailed sheep for viability, motility and intact plasma membrane of spermatozoa
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  8. Reproduction efficiency of artificial insemination beef cattle in east and west district, kabupaten east lombok on 2012
  9. Reproductive efficiency of limousin and simmental cross resulted from artificial insemination (ai) during 2012 in ngoro, jombang
  10. The influence of mangosteen peels infusion (garcinia mangostana l.) to acrosome reaction on rats spermatozoa
  11. Addition of cattle seminal plasma to sperm quality goat on freezing process with time equilibration different
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  14. Suplementation of insuline-like growth factor-i pregnant thoroughbred mare serumon increase of bovine embrio cleavage rates