Planta Husada
ISSN 2338-7130
Vol. 2 / No. 1 / Published : 2014-04
Order : 1, and page :1 - 4
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Original Article :
Eluent effect on sinensetin in orthosiphon stamineus benth. leaves extract
Author :
- Lusiana Arifianti, Rice Disi Oktarina, Idha Kusumawati*1
- Lusiana Arifianti*2
- Rice Disi Oktarina*3
- Idha Kusumawati*4
- Dosen Fakultas Farmasi
- Dosen Fakultas Farmasi
- Dosen Fakultas Farmasi
- Dosen Fakultas Farmasi
Abstract :
Orthosiphon stamineus Benth. is a medicinal plant which grown in Southest Asia and cultivated in Indonesia. The leaves of this plant are used by name of “kumis kucing” in traditional medicine as a diuretics, rheumatisme, urinary tract infection, kronic and acute kidney infection and billiary lithiasis. This study was aimed to determinate the content of sinensetin on varying hidroalcoholic eluent (96%, 70%, 50% ethanol and water). Analitytical method for the determination of sinensetin using by a thin layer chromatography-densitometry method. TLC densitometry quantification is performed by external standard on silica gel plates GF 254. Selectivity tested results that using chloroform-etyl acetate (15:1) as developing solvent and UV detection at 338 nm (top peak), showed a component of sinensetin (Rfstd = 0,39; Rf sampel=0,38) and an identic spectra (MF = 0,99817). The %yield of sinensetin in extract from Orthosiphon stamineus Benth. leaves on each eluen were 1,9% ; 1,3%; 1,1% and 0,3% (ethanol 96%, 70%, 50% and water ) respectively. The highest consentration of sinensetin was in ethanol 96%. From the present study, it may be concluded that ethanol 96% is the best eluent for manufacture of herbal medicine from Orthosiphonis folium.
Keyword :
Orthosiphon stamineus Benth, sinensetin, TLC densitometry,
References :
Wijesekera,(1991) The Medicinal Plant Industry Washington DC : CRC Press
Awale S, Tesuka Y, Banskota A.H, Kouda K, Tun KM, Kadota S,(2001) Five Novel Highly Oxigenated Diterpenes of Orthosiphon stamineus from Myanmar - : Journal of Natural Product
Depkes RI,(2000) Parameter Standar Umum Ekstrak Tumbuhan Obat Jakarta : Depkes RI
Hossain MA, Ismail Z, Rahman A, and Kang SC,(2008) Chemical composition and anti-fungal properties of the essential oils and crude extracts of Orthosiphon stamineus - : Industrial Crop and Product
TaTakeda Y, Matsumoto T, Terao H, Shingu T, FutatsuishiY, Nohara T, Kajimoto T, ,(1993) Orthosiphol D and E, Minor Diterpens from Orthosiphon stamineus. - : Phytochemistry
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Volume : 2 / No. : 1 / Pub. : 2014-04 |
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