UNIVERSITAS AIRLANGGA



Detail Article

Planta Husada

ISSN 2338-7130

Vol. 2 / No. 1 / Published : 2014-04

Order : 1, and page :1 - 4

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Original Article :

Eluent effect on sinensetin in orthosiphon stamineus benth. leaves extract

Author :

  1. Lusiana Arifianti, Rice Disi Oktarina, Idha Kusumawati*1
  2. Lusiana Arifianti*2
  3. Rice Disi Oktarina*3
  4. Idha Kusumawati*4
  1. Dosen Fakultas Farmasi
  2. Dosen Fakultas Farmasi
  3. Dosen Fakultas Farmasi
  4. Dosen Fakultas Farmasi

Abstract :

Orthosiphon stamineus Benth. is a medicinal plant which grown in Southest Asia and cultivated in Indonesia. The leaves of this plant are used by name of “kumis kucing” in traditional medicine as a diuretics, rheumatisme, urinary tract infection, kronic and acute kidney infection and billiary lithiasis. This study was aimed to determinate  the content of sinensetin on varying hidroalcoholic eluent (96%, 70%, 50% ethanol and water). Analitytical method for the determination of sinensetin using by a thin layer chromatography-densitometry method. TLC densitometry quantification is performed by external standard on silica gel plates GF 254. Selectivity tested results that using chloroform-etyl acetate (15:1) as developing solvent and UV detection at 338 nm (top peak), showed a component of sinensetin (Rfstd = 0,39; Rf sampel=0,38) and an identic spectra (MF = 0,99817). The %yield of sinensetin in extract from Orthosiphon stamineus Benth. leaves on each eluen were 1,9% ; 1,3%; 1,1% and 0,3% (ethanol 96%, 70%, 50% and water ) respectively. The highest consentration of sinensetin was in ethanol 96%. From the present study, it may be concluded that ethanol 96% is the best eluent for manufacture of herbal medicine from Orthosiphonis folium.

Keyword :

Orthosiphon stamineus Benth, sinensetin, TLC densitometry,


References :

Wijesekera,(1991) The Medicinal Plant Industry Washington DC : CRC Press

Awale S, Tesuka Y, Banskota A.H, Kouda K, Tun KM, Kadota S,(2001) Five Novel Highly Oxigenated Diterpenes of Orthosiphon stamineus from Myanmar - : Journal of Natural Product

Depkes RI,(2000) Parameter Standar Umum Ekstrak Tumbuhan Obat Jakarta : Depkes RI

Hossain MA, Ismail Z, Rahman A, and Kang SC,(2008) Chemical composition and anti-fungal properties of the essential oils and crude extracts of Orthosiphon stamineus - : Industrial Crop and Product

TaTakeda Y, Matsumoto T, Terao H, Shingu T, FutatsuishiY, Nohara T, Kajimoto T, ,(1993) Orthosiphol D and E, Minor Diterpens from Orthosiphon stamineus. - : Phytochemistry





Archive Article

Cover Media Content

Volume : 2 / No. : 1 / Pub. : 2014-04
  1. Eluent Effect On Sinensetin In Orthosiphon Stamineus Benth. Leaves Extract
  2. Ethyl Acetate Fraction Of Andrographis Paniculata Nees Increases Cytotoxic Effect Of Vincristine On Human Cancer Cell Lines
  3. Antiangiogenesis From Pericarp Of Mangosteen On T47d Breast Cancer
  4. Ethyl Acetate Fraction Of Johar Leaves (cassia Siamea Lamk) On Plasmodium Falciparum Strain G2300 (chloroquin Resistence) In Vitro As An Antimalaria Activity
  5. Pinostrobin Isolated From Kaempferia Pandurata Roxb Induced Apoptosis In T47d Human Beast Cancer Cell Line