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Veterinaria Medika

ISSN 1979-1305

Vol. 3 / No. 1 / Published : 2010-02

TOC : 11, and page :53 - 56

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Original Article :

Addition of growth differentiation factor-9 (gdf-9) at culture medium in vitro on chromosome profile of bovine's oocyte

Author :

  1. Widjiati*1
  2. Nunung Aji Wibowo*2
  3. Indah Norma Triana*3
  1. Dosen Fakultas Kedokteran Hewan
  2. Dosen PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

Growth Differentiation Factor - 9 (GDF-9) was a member of the TGF-â superfamily known to regulatedovarian functions in mammals. The aim this research was to find out the influenced suplementation GDF-9 towardmaturation degree of bovine's oocyte in vitro. The objective of the current study was to test the hypothesis influencesuplementation GDF-9 protein at the oocyte's maturation medium in vitro toward degree's maturation bovine'soocytes. Oocytes were collected with aspiration technique for the folicles wich have 3 – 5 mm diameters. Furthermoreoocytes were matured within TCM 199 medium in wich additional amount 3% BSA, 50 μg / ml gentamycin sulfat,1μg/ml FSH, 1μg/ml LH, 5 ng GDF-9, then oocytes cultured during 22 hours at 38,5º C temperature in the incubatorwith 5% CO2. After 22 hours incubated later doing investigation oocyte maturation with 1 % aceto orcein dye forobserving figure M II. The result showed that Suplementation GDF-9 at a maturation medium gave influenced towardmaturation oocyte which is indicated incrase of number M II and showed significant different.

Keyword :

Metafase II (M II), protein GDF-9, bovine's oocyte, ., .,


References :

  1. Barry E.G. and Perkins D.D., (2001). How to prepare aceto-orcein squashes, especially for pachytene chromosomes. San Diego : Academic Press
  2. Hunter,R.H.F., (2005). Fisiologi dan Teknologi Reproduksi Hewan Betina Domestik. Bandung and Denpasar : Penerbit ITB dan Penerbit Universitas Udayana


   


Archive Article

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Volume : 3 / No. : 1 / Pub. : 2010-02
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  2. Isolation, identification and characterization of inhibin b protein of rattus norvegicus sertoli cell culture as male hormonal contraception candidate
  3. Protective test immunogenic protein of outher membrane salmonella pullorum agains broiler
  4. Profile of combination ketamin xylazine and ketamin midazolam toward physiologycal change in male rabbit
  5. The feed's potential as the contamination source of aspergillus spp. which caused aspergillosis on fowl
  6. Metabolic product rumen fluid of sheep which given lactic acid bacteria suspension and yeast on king grass and rice straw
  7. Addition time interval effect of temu ireng (curcuma aeruginosa roxb.) extract to male mice (mus musculus) liver histopathological description
  8. Path analysis of mastitis on dairy cows at cooperative dairy farm in pasuruan, east java
  9. Viability and motility muscovy drake's (cairina moschata) spermatozoa in a diluter combination of skim milk, fructose and egg yolk
  10. Addition of growth differentiation factor-9 (gdf-9) at culture medium in vitro on chromosome profile of bovine's oocyte
  11. The potency of a black soybean milk and yellow soybean milk on the level of triglycerides in rat (rattus novergicus) fed with a high fat diet
  12. The effect of centrifugation on quality of motility, viability and acrosom cap of goat sperm
  13. Benzopyrene induce apoptosis of the lung cells in ratus norvegicus
  14. Partenogenetik development of mouse oocytes activation using ethanol and 6-dmap by in vitro
  15. Identification of pregnancy specific protein b (pspb) from placenta foetalis (cotyledon) freishian holstein cows