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Veterinaria Medika

ISSN 1979-1305

Vol. 3 / No. 1 / Published : 2010-02

TOC : 11, and page :53 - 56

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Original Article :

Addition of growth differentiation factor-9 (gdf-9) at culture medium in vitro on chromosome profile of bovine's oocyte

Author :

  1. Widjiati*1
  2. Nunung Aji Wibowo*2
  3. Indah Norma Triana*3
  1. Dosen Fakultas Kedokteran Hewan
  2. Dosen PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

Growth Differentiation Factor - 9 (GDF-9) was a member of the TGF-â superfamily known to regulatedovarian functions in mammals. The aim this research was to find out the influenced suplementation GDF-9 towardmaturation degree of bovine's oocyte in vitro. The objective of the current study was to test the hypothesis influencesuplementation GDF-9 protein at the oocyte's maturation medium in vitro toward degree's maturation bovine'soocytes. Oocytes were collected with aspiration technique for the folicles wich have 3 – 5 mm diameters. Furthermoreoocytes were matured within TCM 199 medium in wich additional amount 3% BSA, 50 μg / ml gentamycin sulfat,1μg/ml FSH, 1μg/ml LH, 5 ng GDF-9, then oocytes cultured during 22 hours at 38,5º C temperature in the incubatorwith 5% CO2. After 22 hours incubated later doing investigation oocyte maturation with 1 % aceto orcein dye forobserving figure M II. The result showed that Suplementation GDF-9 at a maturation medium gave influenced towardmaturation oocyte which is indicated incrase of number M II and showed significant different.

Keyword :

Metafase II (M II), protein GDF-9, bovine's oocyte, ., .,

References :

  1. Barry E.G. and Perkins D.D., (2001). How to prepare aceto-orcein squashes, especially for pachytene chromosomes. San Diego : Academic Press
  2. Hunter,R.H.F., (2005). Fisiologi dan Teknologi Reproduksi Hewan Betina Domestik. Bandung and Denpasar : Penerbit ITB dan Penerbit Universitas Udayana


Archive Article

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Volume : 3 / No. : 1 / Pub. : 2010-02
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  9. Viability and motility muscovy drake's (cairina moschata) spermatozoa in a diluter combination of skim milk, fructose and egg yolk
  10. Addition of growth differentiation factor-9 (gdf-9) at culture medium in vitro on chromosome profile of bovine's oocyte
  11. The potency of a black soybean milk and yellow soybean milk on the level of triglycerides in rat (rattus novergicus) fed with a high fat diet
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