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Veterinaria Medika

ISSN 1979-1305

Vol. 7 / No. 2 / Published : 2014-07

TOC : 7, and page :140 - 145

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Original Article :

Analysis of immunogenicity on inactivated dengue virus (denv-1, denv-2, denv-3, denv-4) in mices (mus musculus) as a candidate dengue coctail vaccine

Author :

  1. Deka Uli Fahrodi*1
  2. Helen Susilowati*2
  3. Deya Karsari*3
  4. Eryk Hendrianto*4
  5. Sri Agus Soedjarwo*5
  6. Mufasirin*6
  7. Setiawan Koesdarto*7
  8. Didik Handijanto*8
  9. Fedik Abdul Rantam*9
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Institut Tropical Disease Universitas Airlangga
  3. Institut Tropical Disease Universitas Airlangga
  4. Institut Tropical Disease Universitas Airlangga
  5. Dosen Fakultas Kedokteran Hewan
  6. Dosen Fakultas Kedokteran Hewan
  7. Dosen Fakultas Kedokteran Hewan
  8. Dosen Fakultas Kedokteran Hewan
  9. Dosen Fakultas Kedokteran Hewan

Abstract :

Dengue virus (DENV) is a single stranded RNA virus that circulates in the body of primate and causes DHF human. Dengue virus have four serotypes (DENV-1, DENV-2, DENV-3, DENV-4). Four dengue virus serotypes do have a similar, yet distinct antigenic properties. Infection only one serotype will give immunity to one serotype is concerned, but does not provide cross- immunity. This study aims to determine the immunogenicity of dengue virus (DENV-1, DENV-2, DENV-3 and DENV-4) inactive as a candidate dengue cocktail vaccine. This study used 18 mices were divided into three treatment groups, P0 immunized 0.5 cc PBS, P1 immunized with cocktail of dengue virus (DENV-1, DENV-2, DENV-3 and DENV-4) inactive as much as 0.5 cc TCID50107, P2 immunized cocktail of dengue virus (DENV-1, DENV-2, DENV-3 and DENV-4) inactive as much as 0.5 cc TCID50108. At day 0,7,14 and 21 days of 18 mice that had been treated blood drawn to determine the levels of immunoglobulin M and G with indirect elisa method. On day 28, the treatment group P1 and P2 in the challenge test with a cocktail immunized with dengue virus (DENV-1, DENV-2, DENV-3 and DENV-4) active 0.5 cc TCID50108 for the presence of viremia. The results of this study are significant differences (p<0.05) between the groups P0 (control), P1 and P2 in terms of inducing titers of immunoglobulin (IgM, IgG, IgG1a, IgG2a, and IgG2b). In the challenge test using active dengue virus (DENV-1, DENV-2, DENV-3 and DENV-4) TCID50108 in experimental animals (P1 and P2), then the PCR test followed by electrophoresis results showed the absence of viremia or viral dengue as evidenced by a negative PCR result.

Keyword :

DENV, Imunoglobulin, Challenge test, Vaksin dengue,

References :

  1. Chen, R. and N. Vasilakis, (2011). Dengue-Quo tu et quo vadis. . : Viruses. 3: 1562-1608.
  2. Liu CC, Lei and Yeh, (2008). Imuno-pathogenesis of dengue virus infection. . : J Biomed Sci 8:377-388
  3. Savage, H.M., C.L. Fritz, D. Rutstein, A. Yolwa, V. Vorndam, and D.J. Gubler, (1998). Epidemic of Dengue-4 Virus in Yap State, Federated States of Micronesia, and Implication of Aedes hensili As an Epidemic Vector. USA : Am. J. Tropical Medicine and Hygiene. 58(4): 519-524


Archive Article

Cover Media Content

Volume : 7 / No. : 2 / Pub. : 2014-07
  1. The influence of carbofuran exposure toward white pulp diameter of spleen of mice (mus musculus)
  2. Antibacterial activity of the supernatant of soil isolate bacillus subtilis against aeromonas hydrophila and staphylococcus aureus (in vitro)
  3. The potential of giving synbiotic in different ages of female broilers on histological of ileum
  4. Evaluation toluidine blue staining to identify connective tissue mast cells (ctms) in paraffin block thin skin of dog
  5. Effect using a combination of mpa (medroxy progesterone acetate) and prostaglandin (pgf2α) injection on the percentage of estrous and pregnant on sheeps
  6. Spesifisity test with dot blotting of epidermal growth factor (egf) isolated from cumulus oocyte complex after in vitro maturation
  7. Analysis of immunogenicity on inactivated dengue virus (denv-1, denv-2, denv-3, denv-4) in mices (mus musculus) as a candidate dengue coctail vaccine
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  10. The effect of cosmos caudatus leaf ethanol extract on paracetamol induced in histopathologic liver of (mus musculus) balb / c male
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  14. Cloning gene fragments non-structural 1 (ns1) of dengue virus subtype 1 (denv-1) as a material candidate of vaccine chimera
  15. Effect of vitamin e (α–tocopherol) on the number of leydig cell in mice treated with 2,3,7,8–tetrachlorodibenzo-p-dioxin (tcdd)