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Veterinaria Medika

ISSN 1979-1305

Vol. 7 / No. 2 / Published : 2014-07

TOC : 2, and page :106 - 113

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Original Article :

Antibacterial activity of the supernatant of soil isolate bacillus subtilis against aeromonas hydrophila and staphylococcus aureus (in vitro)

Author :

  1. Erni Rosilawati Sabar Iman*1
  2. Mirni Lamid*2
  3. Madya Adi Waskita*3
  1. Dosen Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Mahasiswa Fakultas Kedokteran Hewan

Abstract :

The aim of this research was to know the antibacterial activity (Minimum Inhibitory Concentration and Minimum Bactericidal Concentration) of supernatant of soil isolate Bacillus subtilis against Aeromonas hydrophila and Staphylococcus aureus and compare the antibacterial activity both of them. Antibacterial compound from B. subtilis are subtilin, subtilosin A, TasA, sublancin, circulin, polymixin and colistin which effective against Gram positive and Gram negative bacteria. Microdilution method was used to evaluate the antibacterial activity from supernatant of Bacillus subtilis by determining MIC and MBC. Concentration range was from 100% - 10%. The result showed the range of MIC and MBC values against Aeromonas hydrophila were 60% - 70% and 70% - 80%. Meanwhile, the range of MIC and MBC values against Staphylococcus aureus were 80% - 90% and 90% - 100%. Statistical analyzed showed that there were highly significant difference of antibacterial activity of supernatant of soil isolate Bacillus subtilis against Aeromonas hydrophila as Gram negative and Staphylococcus aureus as Gram positive bacteria.

Keyword :

Bacillus subtilis, Antibacterial activity, Aeromonas hydrophila, Staphylococcus aureus,


References :

  1. Aslim, B., S. Neode and B. Yavuz, (2002). Determination of some properties of Bacillus isolated from soil. Istanbul : Turkish Journal of Biology.41-48.
  2. Atyah, M.A.S., M.Z. Saad and A.S. Zahrah, (2010). First report of methicillin-resistant Staphylococcus aureus from cage-cultured tilapia (Oreochromis niloticus).. . : Veterinary Microbiology 144:502-504
  3. Awais, M., A.A. Shah, A. Hammed and F. Hasan, (2007). Isolation, identification and optimization of Bacitracin produced Bacillus sp. . : Pak. J. Bot., 39(4): 1303-1312


   


Archive Article

Cover Media Content

Volume : 7 / No. : 2 / Pub. : 2014-07
  1. The influence of carbofuran exposure toward white pulp diameter of spleen of mice (mus musculus)
  2. Antibacterial activity of the supernatant of soil isolate bacillus subtilis against aeromonas hydrophila and staphylococcus aureus (in vitro)
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