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Veterinaria Medika

ISSN 1979-1305

Vol. 6 / No. 1 / Published : 2013-02

TOC : 5, and page :21 - 26

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Original Article :

Comparasion of chicken humoral immune response vaccinated with active ibd lv-13ua and lv-14ua under value optical density indirect elisa

Author :

  1. Hasanah, L.K*1
  2. Rantam, F.A*2
  3. Priyandani, Yuni*3
  4. Suwarno*4
  5. Rahardjo, A.P*5
  1. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  2. Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga
  4. Fakultas Kedokteran Hewan Universitas Airlangga
  5. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

Infectious Bursal Disease (IBD) is a disease of chicken caused by Birnavirus. This disease was immunosuppressive and has a mortality rate up to 100%. This study was aimed to prove the existence of differences in humoral immune response in chicken vaccinated with active IBD vaccine LV-13UA and LV-14UA based on the value of Optical Density with Indirect ELISA method. The study was conducted on 21 chicks that were divided into 3 groups (P0, P1, and P2). Group P1 consist of 7 chicks was vaccinated with active IBD mild strain vaccine at the age of three weeks given 0,3 ml/chick then boostered with active IBD vaccine LV-13UA at the age of five weeks. Group P2 consist of 7 chickswas vaccinated with active IBD mild strain vaccine at  the age of three weeks given 0,3 ml/chick virus then boostered with active IBD vaccine LV-14UA at the age of five weeks. Group P0 (control) consist of 7 chicks was given 0,3 ml normal saline/chick at the age three and five weeks.Blood sampling for value of Optical Density (OD) IBD antibody observations performed three timesin all age groups for three weeks, five weeks, and seven weeks. Measurement of value of Optical Density (OD) IBD antibody was using indirect ELISA method. Data analysis used the General LinearModels (GLM) and ANOVA. The result showed that there are differences in humoral immuneresponse in chicken vaccinated with active IBD vaccine LV-13UA and LV-14UA based on the valueof Optical Density. The value of OD antibody of chicken vaccinated with active IBD vaccine LV14UA is higher than IBD vaccine LV-13UA.

Keyword :

Humoral immune response, Optical Density, Indirect ELISA, IBD vaccine,


References :

  1. Cardoso, W.M., J.L.C. Aguian Filho., J.M. Ramao., R.P.R. Salles., S.R. Camara., A.A. Siquiera., W.F., (2006). Interfence of Infectious Bursal Disease Virus on Antibody Production against Newcastle Disease and Infectious Bronchitis Virus.. Brazil : Brazillian of Poultry Science. v.8/n.3/177-182.
  2. Rudd, M.F., H.G Heine., S.I. Sapats., L. Parede and J. Ignjatovic., (2002). Characterisation of an Indonesian Very Virulent Strain of Infectious Bursal Disease Virus. United States : Eleventh Edition. Iowa State Press.


   


Archive Article

Cover Media Content

Volume : 6 / No. : 1 / Pub. : 2013-02
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  2. Injection effect of mice (mus musculus) testes and spleen homogenat derived hy-antigen on pup’s sex ratio
  3. The colour differences between escherichia coli and pseudomonas aeruginosa colonies in artificial medium that was made from beef extract, mung beans juice, citrate and bromthymol blue
  4. Effects of beef packaging and storage temperature on the early beef spoilage
  5. Comparasion of chicken humoral immune response vaccinated with active ibd lv-13ua and lv-14ua under value optical density indirect elisa
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