Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 6 / No. 2 / Published : 2013-07

Order : 9, and page :121 - 126

Related with : Scholar   Yahoo!   Bing

Original Article :

Comparison of hemocytometer thoma and spectrophotometer method on sperm concentration examination of merino sheep semen

Author :

  1. Sardjito, Trilas*1
  2. Ramayadi, Wira*2
  3. Srianto, Pudji*3
  4. Nidom, C.A*4
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga
  4. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

This research was conducted to determine differences concentration,the semen straw produced from the concentration, and research time of spermatozoa count method with hemocytometer thoma and spectrophotometer of merino sheep semen. One of microscopic examination of semen is calculating the concentration of spermatozoa. Calculating the concentration of spermatozoa is very important especiallyif the semen is processed into frozen semen. Both of these methods are excellent in calculating the concentration of spermatozoa. There are 10 merino sheep semen samples were calculated concentration of spermatozoa. Each sample of semen were counted directly by both methods. After concentration of spermatozoa has been counted, the amount of semen straw produced can be calculated. Statistical T-Test are use to compare the concentration, mini straw produced and research time from two methods. Resultsshowed no significant differences in concentration and semen straw produced results from hemocytometer thoma and spectrophotometer methods, but the significant differences showed in research time from two methods.

Keyword :

Sperm Concentration, Hemocytometer, Spectrophotometer, Merino Sheep, Frozen Semen,

References :

Atiq N., Ullah N., Andrabi S. M. H. and Akhter S.,(2011) Comparison Of Photometer With Improved Neubauer Hemocytometer and Makler Counting Chamber For Sperm Concentration Measurement In Cattle. Pakistan : Pakistan Veteriner Journal. 31:83-84.

Christensen P., Stryhn H., Hansen C.,(2005) Discrepancies in the determination of sperm concentration using Burker-Turk, Thoma and Makler counting chambers. United States : Theriogenology. 63:992– 1003.

Archive Article

Cover Media Content

Volume : 6 / No. : 2 / Pub. : 2013-07
  1. The Influence Of Bacteriocin As A Preservation Of Beef To Extend Storage At 4°c-
  2. Influence Of Infusa Mangosteen Peel (gracinia Mangostana L) For Blood Glucose Levels Of Hyperglycemic Rats
  3. The Potential Of Red Roses Extract (rosa Damascena Mill) As An Antiseptic Stomatitis In Snakes (python Reticulatus) On The Total Number Leukocyte And Defferential Counting Of Leukocytes
  4. The Effect Of Green Bitter Melon Fruit Flesh (momordica Charantia L.) Extract On Mice (mus Musculus) Estrous Cycle Which Superovulated With Pmsg And Hcg
  5. The Antibiotica Susceptibility Of Escherichia Coli Isolated From Etawa Goat Milk
  6. Reproductive’s Efficiency Of Madura Cattle Artificial Insemination Result At Sumenep Regency In 2012
  7. Digestibility Value Of Proteinin Formula Feed For The Cats (felis Silvestris Catus)
  8. Effect Of Sambiloto (andrographis Paniculata) Extract Based On Histopathological Changes Of Gouramy (osphronemus Gouramy) Villi Intestine Infected Aeromonas Hydrophila
  9. Comparison Of Hemocytometer Thoma And Spectrophotometer Method On Sperm Concentration Examination Of Merino Sheep Semen
  10. Profil Protein Hemaglutinin (ha) Berdasarkan Berat Molekul Virus Avian Influenza Isolat Lokal
  11. The Effect Of Borax To Duodenal Histopathological Changes In Rats (rattus Norvegicus)
  12. Giving Probiotics To Increase Milk Production And Milk Proteins Of Dairy Cattle In Gresik Randu Padangan Village
  13. Differential Time Of Artificial Insemination On The Presentage Regnancy Of Fat Tailed Sheep
  14. Pengaruh Vaksinasi Dengue Multivalen Terhadap Ekspresi Toll-like Receptor Dan Sel T Cd4+ Pada Kelinci ( White New Zealand )
  15. Quality Test Of Frozen Semen Of Etawa Goat-breed’s In Three Different Kinds Of Extender With Water Incubator Examination