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Veterinaria Medika

ISSN 1979-1305

Vol. 7 / No. 3 / Published : 2014-01

TOC : 3, and page :216 - 223

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Original Article :

Comparison of sperm quality after separation x and y chromosome between column albumin method and electric separating sperm (ess) method on ekor gemuk sheep

Author :

  1. Ragil Angga Prastya*1
  2. Amung Logam Saputra*2
  3. Siti Zainab*3
  4. Herry Agoes Hermadi*4
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Mahasiswa Fakultas Kedokteran Hewan
  3. Mahasiswa Fakultas Kedokteran Hewan
  4. Dosen Fakultas Kedokteran Hewan

Abstract :

This aims of this study was to determine quality of the sperm (motiltias, vitality, and integrity of membranes) spermatozoa and the size of the sperm head after undergoing a process of separation of sheep chromosome X and Y with two different methods, namely albumin column method using blood serum and Bovine Serum Albumin sheep (BSA) and electric separating sperm (ESS) methods. The study consisted of 6 treatments, namely P0, P1, P2, P3, P4, P5 and each treatment consisted of ten replicates. Data obtained on the quality and size of the sperm head of sheep were analyzed and statistically tested using ANOVA followed by Tukey 5% significance. The results showed that the blood serum of sheep and BSA did not significantly affect the quality of spermatozoa sheep, the most tangible result is shown by the control treatment. Size X and Y spermatozoa head on P1 and P2 showed significant differences to the P0, P1 ratio on chromosome X and Y spermatozoa was 28%: 72% and 32% in comparison P2: 68%. In the second method, namely electric separating sperm (ESS), the results showed that the quality of the sperm cells in test microscopic observation of motility in all treatment groups showed no significant differences, while the power of life and abnormalities observation results show that the results between the P3 and P4 control do not have significant differences but significantly different from P5

Keyword :

Sperm separation, Column albumin method, Electric Separating Sperm, Sperm quality, Size of head sperm,


References :

  1. Sugiarti,T., E. Triwulanningsih, P. Situmorang, R.G. Sianturidan, dan D. A. Kusumaningrum, (2004). Penggunaan katalase dalam produksi semen dingin sapi.. Bogor : Pros. Seminar Nasional Teknologi Peternakan dan Veteriner. Bogor. 4-5 Agustus 2004. Puslitbang Peternakan, 195-205.
  2. Priatmoko, E., (2008). Motilitas dan daya hidup serta abnormalitas spermatozoa sapi friesian holstein setelah pemisahan dengan teknik column albumin menggunakan putih telur. Surabaya : Skripsi. Fakultas Kedokteran Hewan Universitas Airlangga
  3. Mahaputra, L. dan I. Mustofa, (2002). Pemisahan sel sperma pembawa kromosom kelamin dan perkembangan embrio hasil fertilisasi in vitro pada sapi madura.. Surabaya : Media Kedokteran Hewan. 18(1) : 8-10.


   


Archive Article

Cover Media Content

Volume : 7 / No. : 3 / Pub. : 2014-11
  1. The accuracy of production equine chorionic gonadotropin (ecg) frozen dry from local horse for increasing the time onset of oestrus in madura cattle
  2. Effect of infusum kopi leaves (coffea robusta lindl.) on blood glucose level on the mice in alloxan induced
  3. Comparison of sperm quality after separation x and y chromosome between column albumin method and electric separating sperm (ess) method on ekor gemuk sheep
  4. Potential of vitamin e on spermatogenic cell number in mice treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd)
  5. The comparison of maturation between bovine oocyte vitrification before and after in vitro maturation
  6. Potency of pediococcus pentosaceus protease on meat tenderness and histological
  7. Effect of vitamin e on decreased of the leydig cell necrosis number in mice treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd)
  8. The immunomodulator potency of combination of andrographis paniculata and echinacea purpurea on total and differential leucocytes count in mice with heat stress
  9. The effect of various egg duck concentration in skim milk as semen diluters of fat-tailed sheep for motility, viability and intact plasma membrane spermatozoa before freezing
  10. Antibacterial effect of mango’s leaves (mangifera indica) extract against staphylococcus aureus and escherichia coli
  11. Effectiveness of honey and the juice of noni (morinda citrifolia linn) as well as mixtures of the number of bacteria on chicken carcasses
  12. The potential of soursop (annona muricata linn.) leaves extract as immunostimulantin rabbit pbmc
  13. Crude protein and crude fiber in rice bran fermented by acidothermus cellulolyticus or aspergillus terreus wpl 109 or wpl 209
  14. Level of contamination of salmonella sp in white prawns (litopenaeus vannamei) sold at some wet and modern market in surabaya
  15. Potensi alkaloid achyranthes aspera linn terhadap ekspresi protein bax sel kanker payudara pada mencit (mus musculus) yang diinduksi benzopyrene