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Veterinaria Medika

ISSN 1979-1305

Vol. 4 / No. 2 / Published : 2011-07

TOC : 11, and page :135 - 138

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Original Article :

Concentration of pregnancy associated glycoprotein (pag) of milk of dairy cows at 21 and 28 days post artificial insemination

Author :

  1. Samik, Abdul*1
  1. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The development of early pregnancy diagnosis method in dairy cows can be undertaken by observing specific substance presents in the milk of pregnant cows, i.e. the Pregnancy Associated Glycoprotein (PAG). The objective of this study was to prove that anti-PAG was able to recognize PAG protein from milk of pregnant dairy cow as the base for developing one of early pregnancy test in dairy cows.This study was conducted by following stages: 1) synchronization of estrus and artificial insemination and 2)identification of  PAG protein in the milk at 21 and 28 days post AI. The result showed that average of concentration of PAG protein in the milk at 21 days post AI are 370,221 µg/ml and 716,317 µg/ml. Based on the results, it can be concluded that dairy cows pregnancy diagnosis with identification of PAG protein at 28 day after AI has more reliable validation value as compared at 21 day after AI.

Keyword :

Estrus synchronization, Artificial insemination, PAG of milk, Pregnancy diagnosis,

References :

  1. El Amiri B., B. Remy, N.M. De Sousa and J.F. Beckers, (2003). Isolation and Characterization of Eight Pregnancy-Associated Glycoprotein Present at Hight Levels in the Cattle Placenta between Day 60 and Day 100 of Gestation.. United States : Reprod Nutr Dev. 44(3): 169-181
  2. Gonzales F., J. Sulon, J.M. Garbayo, M. Batista, F. Cabrera, P.O. Calero, A. Gracia and J.F. Beckers, (2000). Secretory Profiles of Pregnancy-Associated Glycoproteins at Different Stages of Pregnancy in the Cattle.. United States : Reproduction in Domestic Animals. Vol 35. p 79
  3. Vandaele L., S. Verberckmoes, B. El Amiri, J. Sulon, L. Duchateau, A. Van Soom, J.F. Beckers and A. , (2005). Use of a Homologuos Radioimmunoassay (RIA) to Evaluate the Effect of Maternal and Foetal Parameters on PregnancyAssociated Glycoprotein (PAG) Concentration in Cattle. United States : Theriogenology. 63(7): 1914-1924


Archive Article

Cover Media Content

Volume : 4 / No. : 2 / Pub. : 2011-07
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  10. Antibody response and protectivity in chickens after vaccination with nd lv12 active vaccine
  11. Concentration of pregnancy associated glycoprotein (pag) of milk of dairy cows at 21 and 28 days post artificial insemination
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