UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 4 / No. 3 / Published : 2011-11

Order : 8, and page :197 - 202

Related with : Scholar   Yahoo!   Bing

Original Article :

Difference optical density405nm value antibody of layer vaccinated with infectious bronchitis active monovalent and infectious bronchitis activebivalent (ib-nd) by using indirect elisa

Author :

  1. Suwarno*1
  2. Mega Kusuma Dewi*2
  3. Fedik. A Rantam*3
  4. Yuni Priyandani*4
  1. Dosen Fakultas Kedokteran Hewan
  2. Mahasiswa Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan
  4. Fakultas Farmasi Unair

Abstract :

Infectious bronchitis (IB) is caused by a coronavirus. The aim of this study was determine value of Optical Density (OD) IB antibody difference on layer chicken vaccinated with IB active monovalent vaccine and IB-ND active bivalent vaccine. A total of 21 chicks were divided into three groups and given 0.3 ml/chicken doses of vaccine. Group 1 (P1), seven chickens were vaccinated with IB active monovalent at the age of two weeks with a dose of 104,23 EID50/ml (Egg Infective Dose) virus. Group 2 (P2), seven chickens was vaccinated with IB-ND active bivalent at the age of two weeks with a dose of IB 102,5 EID50/ml, and ND 106,5 EID50/ml. Group 3 (P0), seven chickens as a control given 0.3 ml physiological NaCl /chicken. Blood sampling for value of Optical Density (OD) IB antibody were taken three times in all age groups for two week, four week, and six week. Measurement of value of Optical Density (OD) IB antibody was used indirect ELISA. Data analysis used the General Linear Models (GLM) and MANOVA. The results showed that there is difference value of Optical Density (OD) IB antibody of vaccinated layer chickens with IB active monovalent and IB-ND active bivalen vaccine where the value of OD IB antibody for IB active monovalent vaccine is higher than IB-ND active bivalent vaccine.

Keyword :

IB, ND, Monovalent Vaccine, Bivalent Vaccine, Indirect ELISA,


References :

Butcher, G. D., D. P. Shapiro, R. D. Miles,(2002) Infectious Bronchitis Virus: Classical and Variant Strains . : Vet. Med. Lar. Anm. Cli. Sci Dept. University of Florida. VM127: 1-4

Cardoso, W. M., J. L. C. Aguiar, J. M. Romao, W. F. Oliveira, R. P. R. Salles, R. S. Teiseira and M.,(2005) Effect of Associated Vaccines on The Interference Between Newcastle Disease Virus and Infectious Bronchitis Virus in Broilers . : Rev. Bras. Cienc. Avic. 7(3)

Cardoso, W. M., J. L.C. Aguian, J. M. Ramao., R. P. R. Salles., S. R. Camara., A. A. Siquiera., W .F,(2006) Interfence of Infectious Bursal Disease Virus on Antibody Production against Newcastle Disease and Infectious Bronchitis Virus . : Braz. of Poul. Sci. 8(3): 177-182





Archive Article

Cover Media Content

Volume : 4 / No. : 3 / Pub. : 2011-11
  1. Potential Carbofuran Induced Oxidative Stress, Decreased Choline Esterase And Increased Brain Cell Embryonal Death
  2. Crossbreds Entog And Duck Artificial Insemination Technology Of Diluted And Doses For Fertibily
  3. Pengaruh Pemberian Alkaloid Daun Jarong (achyranthes Aspera Linn) Pada Mencit Yang Terkena Kanker Mammae Terhadap Gambaran Hitung Jenis Leukosit
  4. Motilitas, Persentase Hidup Dan Keutuhan Membran Spermatozoa Domba Ekor Gemuk Post Thawing Dalam Tiga Macam Diluter
  5. The Quality Of Ram Sperm After Washing With Brackett And Oliphant’s (bo) Medium At Skim Milk And Egg Yolk Diluent
  6. Hematology Parameter Of Kacang Goats In Mojosarirejo Village Driyorejo Gresik
  7. The Utilization Of Ginger (zingiber Officinale ) As Natural Antibacterial On Pasteurized Milk Based On The Reduce Number Of Escherichia Coli
  8. Difference Optical Density405nm Value Antibody Of Layer Vaccinated With Infectious Bronchitis Active Monovalent And Infectious Bronchitis Activebivalent (ib-nd) By Using Indirect Elisa
  9. The Use Of Recycle Soybean Fermented Cake (tempe) With Cellulolytic Bacteria As Corn Substitution To Consumption Feed, Egg Production And Feed Convertion Ratio Of Layer Duck
  10. The Prevalence Of Helminthiasis Gastrointestinal Tract Of Sumatran Tiger (panthera Tigris Sumatrae) And Bengal Tiger (panthera Tigris Tigris) In Three Different Areas Of Conservation
  11. Supplement Of Acrosin To The Post Thawing Sperm Of Half-breed Of Etawa Goat (pe) Towards Capacitation And Acrosome Reaction Of Spermatozoa
  12. The Effectiveness Of Glutaraldehyde And Didecyl Dimethyl Ammonium Chloride Combination As A Disinfectant To Decrease Total Number Of Bacteria On Layer Chicken Cages
  13. Anti Hy Preparation As Raw Material For Frozen Semen And Frozen Female Embryos
  14. Aphrodisiac Bioscreening Models, Profile Of Justicia Gendarussa Burm.f., Pimpinella Pruatjan Molkenb And Pangium Edule Reinw. Extracts On Isolated Frog Gastric Smooth Muscle
  15. Application Of Human Menopose Gonadotrophin (hmg) Toward Follicle Growth In Cows Ovary Hypofunction

Information VETMED