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Veterinaria Medika

ISSN 1979-1305

Vol. 7 / No. 2 / Published : 2014-07

TOC : 13, and page :178 - 183

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Original Article :

Effect of mangosteen (garcinia mangostana l.) pericarp extract on tlr5 and cd14 expression in immunized mice against newcastle disease vaccine

Author :

  1. Demas Moch. Zain Fithronny*1
  2. Rochmah Kurnijasanti*2
  3. Fedik Abdul Rantam*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

Mangosteen pericarp has well-recognized for reducing the incidence of degenerative diseases including cancer, heart disease, inflammation, arthritis, and immune system. The main chemical substance of mangosteen pericarp that role in improving health is the xanthone which is belonged to phenolic acid that has been studied for its remarkable biological activities in recent years. The research was conducted to analyze the immunomodulatory effect of mangosteen pericarp extract (MPE), by measuring both the expression of TLR5 and CD14 in mice PBMCs. Animal used in the research were 36 female Balb/c mice, that randomly separated into six groups (T0, T1, T2, T3, T4, T5) with six animal each. T0 was negative control group, TI was administered with 40 mg/ml MPE, T2 until T4 was administered with 20 mgml, 40 mg/ml, 60 mg/ml respectively, and T5 was positive control group using Stimuno®. All groups were vaccinated by inactive velogenic type ND vaccine except for T1 group once and without booster. Blood collection has been done a week after vaccination. The result indicated that MPE could increase both the activity of TLR5 and CD14 with 40 mg/ml as optimal dose.

Keyword :

Mangosteen pericarp extract, Immunomodulatory effect, Mice, TLR5, CD14,

References :

  1. Bieli C., (2007). Polymorphism in CD14 Modifies the Effect of Farm Milk Consumption on Allergic Diseases and CD14 Gene Expression. . : J Allergy Clin Immunol. 120:1308-1315
  2. Cook D.N., D.S. Pisetsky and D.A. Schwartz, (2004). Toll-like Receptors in the Pathogenesis of Human Disease.. . : Nat Immunol. 5:975-979.
  3. Stanilove, S.A., Z.G. Dobreva, E.S. Slavov and L.D. Mitera, (2005). C3 binding glycoprotein from Cuscuta Europea induced different cytokine profiles from human PBMC compared to other plant and bacterial immunomodulators. . : Int. Immunopharmacol. 5: 723-734. PMID: 15710341


Archive Article

Cover Media Content

Volume : 7 / No. : 2 / Pub. : 2014-07
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  13. Effect of mangosteen (garcinia mangostana l.) pericarp extract on tlr5 and cd14 expression in immunized mice against newcastle disease vaccine
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